LOCUS       sequence-338288-        6084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9C1_STOP
FEATURES             Location/Qualifiers
     source          1..6084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3641..3740)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3757..3776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3758..3776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3781..3797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3781..3797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3899..3918)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3910..4719
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3977..3996)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4866..5454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5355..5374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5608..5625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5784..5811)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5903..5989)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6038..6060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6052..6069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6053..6069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cggcatcttc
      121 aaggagttct ttttctctac cgaggacctg cctgaagtga tcctgaccct gagcctgatc
      181 agctccatcg gcgccttcct gaatcggcac ctggaggatt ttcccatccc tgtgccagtg
      241 atcctgtttc tgctgggctg tagcttcgag gtgctgtcct ttacctctag ccaggtgcag
      301 aggtacgcca acgccatcca gtggatgtcc cccgacctgt ttttccgcat cttcacccct
      361 gtggtgtttt tcaccacagc ctttgacatg gatacataca tgctgcagaa gctgttctgg
      421 cagatcctgc tgatcagcat cccaggcttt ctggtgaatt atatcctggt gctgtggcac
      481 ctggcctccg tgaaccagct gctgctgaag cccacccagt ggctgctgtt ctctgccatc
      541 ctggtgtcct ctgaccctat gctgacagca gcagcaatca gggatctggg actgtccaga
      601 tctctgatca gcctgatcaa tggcgagtcc ctgatgacct ctgtgatcag cctgatcacc
      661 ttcacaagca tcatggactt tgatcagagg ctgcagtcca agcgcaacca cacactggcc
      721 gaggagatcg tgggcggcat ctgctcttac atcatcgcca gcttcctgtt tggcatcctg
      781 agctccaagc tgatccagtt ctggatgtcc accgtgtttg gcgacgatgt gaatcacatc
      841 tctctgatct tcagcatcct gtacctgatc ttttatatct gtgagctggt gggcatgtct
      901 ggcatcttca ccctggccat cgtgggcctg ctgctgaaca gcacatcctt taaggccgcc
      961 atcgaggaga ccctgctgct ggagttctgg acatttctgt cccggatcgc cttcctgatg
     1021 gtgtttacct ttttcggcct gctgatcccc gcccacacat acctgtatat cgagttcgtg
     1081 gatatctact atagcctgaa tatctatctg accctgatcg tgctgagatt tctgacactg
     1141 ctgctgatct cccccgtgct gtctagggtg ggccacgagt tctcttggcg ctggatcttt
     1201 atcatggtgt gcagcgagat gaagggcatg cctaacatca atatggccct gctgctggcc
     1261 tactccgacc tgtatttcgg ctccgataag gagaagtctc agatcctgtt tcacggcgtg
     1321 ctggtgtgcc tgatcaccct ggtggtgaac aggttcatcc tgcctgtggc agtgacaatc
     1381 ctgggactga gggacgccac cagcacaaag tacaagtccg tgtgctgtac cttccagcac
     1441 tttcaggagc tgacaaagtc cgccgcctct gccctgaagt ttgacaagga tctggccaac
     1501 gccgattgga atatgatcga gaaggccatc accctggaga acccttatat gctgaatgag
     1561 gaggagacca cagagcacca gaaggtgaag tgcccacact gtaacaagga gatcgacgag
     1621 atcttcaata ccgaggccat ggagctggcc aaccggagac tgctgtctgc ccagatcgcc
     1681 agctaccagc ggcagtatag aaatgagatc ctgtctcaga gcgccgtgca ggtgctggtg
     1741 ggagcagcag agtccttcgg cgagaagaag ggcaagtgca tgtctctgga caccatcaag
     1801 aactactccg agtctcagaa gaccgtgaca tttgcccgga agctgctgct gaactgggtg
     1861 tacaatacaa gaaaggagaa ggagggccca agcaagtact tcttcttcag gatctgtcac
     1921 accatcgtgt tcacagagga gtttgagcac gtgggctacc tggtcatcct gatgaatatc
     1981 ttccccttta tcatcagctg gatctcccag ctgaatgtga tctatcactc cgagctgaag
     2041 cacaccaact actgcttcct gacactgtat atcctggagg ccctgctgaa gatcgccgcc
     2101 atgcgcaagg acttctttag ccacgcctgg aatatctttg agctggccat caccctgatc
     2161 ggcatcctgc acgtgatcct gatcgagatc gataccatca agtacatctt caacgagaca
     2221 gaagtgatcg tgttcatcaa ggtggtgcag ttctttagga tcctgcgcat cttcaagctg
     2281 atcgccccaa agctgctgca gatcatcgat aagaggatgt cccaccagaa gaccttttgg
     2341 tacggcatcc tgaagggcta tgtgcagggc gaggccgaca tcatgaccat catcgatcag
     2401 atcacatcta gcaagcagat caagcagatg ctgctgaagc aggtcatccg caacatggag
     2461 cacgccatca aggagctggg ctacctggag tatgaccacc ccgagatcgc cgtgaccgtg
     2521 aagacaaagg aggagatcaa cgtgatgctg aatatggcca ccgagatcct gaaggccttc
     2581 ggcctgaagg gcatcatcag caagacagag ggcgccggca tcaataagct gatcatggcc
     2641 aagaagaagg aggtgctgga tagccagtcc atcatcagac cactgaccgt ggaggaggtg
     2701 ctgtaccaca tcccctggct ggacaagaac aaggattata tcaatttcat ccaggagaag
     2761 gccaaggtgg tgacattcga ctgcggcaac gatatctttg aggagggcga cgagcctaag
     2821 ggcatctaca tcatcatctc tggcatggtg aagctggaga agagcaagcc aggcctgggc
     2881 atcgaccaga tggtggagag caaggagaag gatttcccca tcatcgacac cgattatatg
     2941 ctgtccggcg agatcatcgg cgagatcaac tgcctgacaa atgagccaat gaagtactcc
     3001 gccacctgca agacagtggt ggagacctgt ttcatcccta agacacacct gtatgatgcc
     3061 tttgagcagt gttctccact gatcaagcag aagatgtggc tgaagctggg actggcaatc
     3121 accgcacgga agatcagaga gcacctgagc tacgaggact ggaactataa tatgcagctg
     3181 aagctgagca atatctacgt ggtggatatc cccatgtcca ccaagacaga catctacgat
     3241 gagaacctga tctatgtgat cctgatccac ggagcagtgg aggactgcct gctgcggaag
     3301 acctatagag ccccattcct gatccccatc acatgtcacc agatccagag catcgaggac
     3361 ttcaccaagg tggtcatcat ccagacccct atcaacatga agacattcag gcgcaatatc
     3421 cggaagtttg tgcctaagca caagtcctac ctgaccccag gcctgatcgg ctctgtgggc
     3481 acactggagg agggcatcca ggaggagcgg aacgtgaagg aggacggagc acactctgcc
     3541 gcaaccgcca gatcccccca gccttgctct ctgctgggca caaagttcaa ttgtaaggag
     3601 agccccagaa tcaacctgag gaaggtgcgc aaggagtaag acccagcttt cttgtacaaa
     3661 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3721 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     3781 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     3841 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     3901 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     3961 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     4021 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     4081 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     4141 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     4201 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     4261 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     4321 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     4381 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     4441 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     4501 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     4561 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     4621 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     4681 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     4741 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     4801 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5521 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5581 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5641 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5701 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     5761 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     5821 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     5881 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     5941 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     6001 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     6061 acggccagtc ttaagctcgg gccc
//
