LOCUS       sequence-338293-        4899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC45A1_STOP
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2456..2555)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2572..2591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2573..2591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2596..2612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2596..2612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2714..2733)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2725..3534
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2792..2811)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3681..4269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4170..4189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4423..4440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4599..4626)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4718..4804)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4853..4875
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4867..4884
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4868..4884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgct gcagcagcca
      121 ggacctaggc caggccgcca gcagccctcc ggcgacaggg atgcatgcag gctgcaccct
      181 cagggcaggc cccctgccct gccaaccatg atccctgcag caagctccac accaccaggc
      241 gacgcactgt tcccaagcgt ggcaccccag gatttttggc gctcccaggt gaccggatac
      301 agcggatccg tgacaaggca cctgtcccac cgcgccaaca acttcaagcg gcaccctaag
      361 cggagaaagt gcatcagacc aagccctcca ccccctccaa acaccccctg tcctctggag
      421 ctggtggact tcggcgatct gcacccccag cggtccttca gagagctgct gtttaatggc
      481 tgcatcctgt tcggcatcga gttttcttac gccatggaga ccgcctatgt gacacccgtg
      541 ctgctgcaga tgggcctgcc tgaccagctg tatagcctgg tgtggttcat ctccccaatc
      601 ctgggctttc tgctgcagcc actgctggga gcatggtctg ataggtgtac cagcagattc
      661 ggcaggcgcc ggccctttat cctggtgctg gcaatcggcg ccctgctggg actgagcctg
      721 ctgctgaacg gaagggacat cggaatcgcc ctggcagatg tgaccggcaa tcacaagtgg
      781 ggactgctgc tgacagtgtg cggagtggtg ctgatggact tcagcgccga ctccgccgat
      841 aacccatctc acgcctacat gatggacgtg tgcagccccg ccgaccagga tagaggcctg
      901 aatatccacg cactgctggc aggactggga ggaggcttcg gctacgtggt gggcggcatc
      961 cactgggata agaccggatt tggacgggcc ctgggaggac agctgagagt gatctatctg
     1021 ttcaccgccg tgacactgtc cgtgaccaca gtgctgacac tggtgtctat cccagagagg
     1081 ccactgaggc caccttctga gaagagggcc gccatgaagt ctcctagcct gccactgcca
     1141 ccaagccctc cagtgctgcc tgaggaggga ccaggcgact ctctgcctag ccacaccgcc
     1201 acaaactttt ctagcccaat ctccccccct tctcccctga cccctaagta cggcagcttc
     1261 atctcccgcg attcctctct gaccggcatc agcgagttcg ccagctcctt tggcacagcc
     1321 aatatcgact ccgtgctgat cgattgcttt acaggaggac acgactccta cctggcaatc
     1381 ccaggatctg tgcctaggcc acccatctcc gtgtctttcc caagggcacc agacggcttt
     1441 tatcggcagg atagaggact gctggaggga agggagggcg ccctgaccag cggctgtgac
     1501 ggcgatatcc tgcgcgtggg ctccctggac acctctaagc ccaggtctag cggcatcctg
     1561 aagcgcccac agacactggc aatcccagat gcagcaggag gaggaggccc tgagaccagc
     1621 agaaggcgca acgtgacatt ctcccagcag gtggccaaca tcctgctgaa tggcgtgaag
     1681 tacgagtccg agctgaccgg atcctctgag agggcagagc agcctctgtc tgtgggcaga
     1741 ctgtgcagca ccatctgtaa catgccaaag gccctgagga cactgtgcgt gaatcacttc
     1801 ctgggctggc tgagctttga gggcatgctg ctgttctata ccgactttat gggcgaggtg
     1861 gtgtttcagg gcgatcctaa ggccccacac acctctgagg cctaccagaa gtataacagc
     1921 ggcgtgacaa tgggctgctg gggcatgtgc atctacgcct tctccgccgc cttttattct
     1981 gccatcctgg agaagctgga ggagttcctg tccgtgcgga ccctgtactt catcgcctat
     2041 ctggcctttg gcctgggaac cggactggcc acactgtcca gaaatctgta cgtggtgctg
     2101 tctctgtgca tcacatatgg catcctgttt agcaccctgt gcacactgcc ctactccctg
     2161 ctgtgcgact actatcagtc taagaagttc gcaggaagct ccgccgacgg aaccaggaga
     2221 ggcatgggcg tggatatctc tctgctgagc tgccagtact ttctggccca gatcctggtg
     2281 agcctggtgc tgggaccact gacaagcgcc gtgggatccg ccaacggcgt gatgtatttc
     2341 tctagcctgg tgtcctttct gggctgtctg tactcctctc tgttcgtgat ctatgagatc
     2401 cctccaagcg acgccgccga tgaggagcac agacctctgc tgctgaatgt gtaagaccca
     2461 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2521 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2581 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2641 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2701 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2761 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2821 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2881 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2941 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3001 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3061 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3121 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3181 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3241 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3301 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3361 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3421 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3481 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3541 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3601 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4561 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4621 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4681 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4741 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4801 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4861 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
