LOCUS       sequence-338294-        4359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC13A3_STOP
FEATURES             Location/Qualifiers
     source          1..4359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1916..2015)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2032..2051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2033..2051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2056..2072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2056..2072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2174..2193)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2185..2994
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2252..2271)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3141..3729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3630..3649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3883..3900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4059..4086)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4178..4264)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4313..4335
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4327..4344
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4328..4344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cgccctggca
      121 gcagcagcca agaaggtctg gtccgcccgg agactgctgg tgctgctgtt taccccactg
      181 gccctgctgc cagtggtgtt cgcactgcca cctaaggagg gcaggtgcct gtttgtgatc
      241 ctgctgatgg ccgtgtactg gtgtaccgag gccctgccac tgtccgtgac agccctgctg
      301 cctatcgtgc tgtttccatt catgggcatc ctgccctcta acaaggtgtg ccctcagtat
      361 ttcctggaca ccaattttct gttcctgagc ggcctgatca tggcctccgc catcgaggag
      421 tggaacctgc acaggcgcat cgccctgaag atcctgatgc tggtgggagt gcagcctgca
      481 aggctgatcc tgggcatgat ggtgaccaca tcttttctga gcatgtggct gtctaacacc
      541 gccagcacag ccatgatgct gcctatcgcc aatgccatcc tgaagtccct gttcggccag
      601 aaggaggtgc gcaaggatcc atcccaggag tctgaggaga acaccgccgc cgtgcggaga
      661 aatggcctgc acaccgtgcc aacagagatg cagtttctgg caagcaccga ggcaaaggac
      721 cacccaggag agacagaggt gccactggac ctgcccgccg attctcggaa ggaggatgag
      781 tacaggcgca atatctggaa gggcttcctg atcagcatcc catatagcgc ctccatcgga
      841 ggaaccgcca cactgaccgg aacagcacca aacctgatcc tgctgggcca gctgaagagc
      901 ttctttcctc agtgcgacgt ggtgaatttt ggctcctggt ttatcttcgc ctttcctctg
      961 atgctgctgt tcctgctggc cggctggctg tggatctctt ttctgtacgg aggactgagc
     1021 ttccggggat ggagaaagaa caagagcgag atcaggacca atgcagagga tagggcaaga
     1081 gccgtgatca gggaggagta tcagaacctg ggccctatca agttcgccga gcaggccgtg
     1141 ttcatcctgt tttgtatgtt cgccatcctg ctgtttaccc gggaccctaa gttcatccca
     1201 ggctgggcct ccctgtttaa tccaggcttc ctgtctgatg ccgtgaccgg cgtggccatc
     1261 gtgacaatcc tgttcttttt cccctcccag agaccttctc tgaagtggtg gttcgacttt
     1321 aaggccccaa acaccgagac agagcccctg ctgacctgga agaaggccca ggagacagtg
     1381 ccatggaata tcatcctgct gctgggagga ggattcgcaa tggcaaaggg atgcgaggag
     1441 agcggactgt ccgtgtggat cggaggacag ctgcacccac tggagaacgt gccacccgcc
     1501 ctggccgtgc tgctgatcac cgtggtcatc gcctttttca cagagtttgc ctctaatact
     1561 gccacaatca tcatcttcct gcccgtgctg gcagagctgg caatcaggct gcgcgtgcac
     1621 cctctgtacc tgatgatccc aggcaccgtg ggctgtagct tcgcctttat gctgcctgtg
     1681 agcacacctc caaactccat cgcctttgcc tctggccacc tgctggtgaa ggacatggtg
     1741 cggaccggcc tgctgatgaa cctgatgggc gtgctgctgc tgagcctggc catgaatacc
     1801 tgggcccaga caatctttca gctgggcaca ttccccgact gggccgatat gtattccgtg
     1861 aacgtgaccg ccctgccccc tacactggcc aatgatacct tcagaacact gtaagaccca
     1921 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1981 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2041 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2101 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2161 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2221 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2281 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2341 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2401 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2461 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2521 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2581 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2641 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2701 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2761 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2821 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2881 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2941 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3001 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3061 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4021 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4081 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4141 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4201 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4261 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4321 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
