LOCUS       sequence-338320-        8212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of miR-ctrl with dTomato as a reporter 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.dTomato.miR-ctrl
FEATURES             Location/Qualifiers
     source          1..8212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    650..883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1068..1112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1325..1442
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1500..1907
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1907..1916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1913..2614
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2046..2064)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2314..2333
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2508..2531
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           2639..2760
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           2788..2802
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           2830..2956
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    2979..3567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3032..3052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3450..3461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3639..3872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3950..4071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3987..4006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4041..4060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4111..4246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4266..4285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4267..4285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4295..4312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4295..4311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4304..4326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4453..4908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4750..4771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4934..5038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5039..5107,5108..5899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5257..5276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6070..6658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6559..6578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6812..6829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6946..6967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6982..6987,6988..7005,7006..7012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7020..7036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7025..7047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7044..7060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7044..7060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7079..7099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7081..7099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7144..7523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        7580..7771
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7603..7623)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7790..8016
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8017..8197
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
       61 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      121 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      181 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      241 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      301 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      361 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      421 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      481 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      541 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      601 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      661 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
      721 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
      781 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
      841 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
      901 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
      961 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1021 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1081 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1141 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1201 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1261 cccacctccc aaccccgagg ggacaattct agatcttgag acaaatggca gtattcatcc
     1321 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     1381 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     1441 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag cgagctgcag
     1501 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
     1561 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     1621 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     1681 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     1741 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     1801 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     1861 acccctcact cggcgcgcca gtcctccgac agactgagtc ggatccgcca ccatggtgag
     1921 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     1981 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2041 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2101 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     2161 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2221 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     2281 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     2341 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     2401 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     2461 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     2521 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     2581 cctgttcctg tacggcatgg acgagctgta caagtgagct agccgtctca gatcctgttt
     2641 gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac acttgctggg
     2701 attacttctt caggttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga
     2761 gcgatctcgc ttgggcgaga gtaagtagtg aagccacaga tgtacttact ctcgcccaag
     2821 cgagagtgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac
     2881 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
     2941 taaattaaat cactttaccg gtaacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     3001 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3061 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3121 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3181 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3241 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3301 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3361 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3421 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3481 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3541 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     3601 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3661 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3841 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     3901 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4081 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4141 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4201 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4261 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4321 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4381 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4441 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4861 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5041 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5101 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5161 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5221 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5281 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5341 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5401 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5461 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5521 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5581 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5641 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5701 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5761 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5821 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5881 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5941 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6001 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6061 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6121 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6181 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6241 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6301 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6361 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6421 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6481 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6541 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6601 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6661 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6721 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6781 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6841 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6901 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6961 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7021 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7081 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcggtaccgt cgacctgcag
     7141 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     7201 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     7261 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     7321 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     7381 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7441 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7501 tattagtcat cgctattacc atggcgcgcc atgcagggcc tgaaataacc tctgaaagag
     7561 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     7621 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7681 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7741 tgcatctcaa ttagtcagca accatagtcc cttaagaggc gcgccttaat gtagtcttat
     7801 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     7861 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     7921 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     7981 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     8041 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8101 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8161 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac ag
//
