LOCUS       sequence-338326-       10341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of murine Arid3a cDNA with GFP as 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.mArid3a.IRES.GFP
FEATURES             Location/Qualifiers
     source          1..10341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             161..341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(342..568)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     enhancer        587..778
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     735..755
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     enhancer        835..1214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1259..1279)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1259..1277)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1298..1314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1298..1314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1311..1333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1322..1338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1346..1352,1353..1370,1371..1376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1391..1412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1529..1546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1700..2288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1780..1799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2459..3250,3251..3319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3082..3101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3320..3424)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3450..3905)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3587..3608)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3799..3818
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4032..4054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4046..4063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4047..4063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4073..4092
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4073..4091
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4112..4247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4287..4408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4298..4317)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4352..4371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4486..4719
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4791..5379
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4897..4908
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5306..5326
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5395..6108,6109..6111,6112..6114))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5398..6108,6109..6111,6112..6114))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5440..5461)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5789..5808
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6048..6069
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(6115..6123,6124..6126,6127..6701)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6288..6307)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6517..6534
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(8581..8987)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    9045..9162
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9375..9419)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9604..9837
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    join(10330..10341,1..114)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
       61 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
      121 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
      181 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
      241 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
      301 gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta tcgagctagg
      361 cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat ccatgtcaga
      421 cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac caatcggcat
      481 gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac catgttgcaa
      541 gactacaaga gtattgcata agactacatt aaggcgcgcc tcttaaggga ctatggttgc
      601 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
      661 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
      721 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagaa
      781 ggtacctaac caagttcctc tttcagaggt tatttcaggc cctgcatggc gcgccatggt
      841 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      901 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      961 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     1021 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     1081 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     1141 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     1201 caataatcaa tgtcaacctg caggtcgacg gtaccgcttg cagctccagc ttttgttccc
     1261 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3421 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     3481 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3541 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3601 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3661 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3721 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3781 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3841 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3901 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3961 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4021 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     4081 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     4141 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4201 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4261 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     4321 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4381 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     4441 gttataaata ctgaataata agatgacatg aactactata cgtactgcta gagattttcc
     4501 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4561 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     4621 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
     4681 gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
     4741 agtggctaag atctacagct gccttgtaag tcattggtct taaagtcgac gcggggaggc
     4801 ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag
     4861 agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca
     4921 gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga
     4981 tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca
     5041 gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
     5101 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
     5161 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
     5221 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
     5281 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
     5341 agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgatcactt
     5401 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     5461 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
     5521 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     5581 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     5641 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     5701 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     5761 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     5821 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     5881 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     5941 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     6001 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     6061 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggttgt
     6121 ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
     6181 gacgtttttt taacctcgac taaacacatg taaagcatgt gtaccgaggc cccagatcag
     6241 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     6301 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     6361 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     6421 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
     6481 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
     6541 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
     6601 aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc
     6661 ggcttcggcc agtaacgtta gggggggggg agggagaggg gcggatcctc agttactcat
     6721 gcatacgcgt tatggcaggg aattgttact ggtggaactc atggtgggag tagaaacgcc
     6781 aggaagtccc acctgcccgc cagagacggt tgacccactg gccccagtgc gacttccggt
     6841 agtggtattt gtaccaatag agctgactcc gcccttacct ggtgctgaag gtgctgtagg
     6901 aggaggaggc tgggcaaaaa ggacgccagt atatactatc ccattcattt caacactcat
     6961 tgaaatacta ttggaaccat ttgcagatgt caaggaaacg gcactatctt ggcggctttc
     7021 ggaagcttgg ctattaattc taatgttcat gggaagttgg gcggtcattg ccagaaagga
     7081 ctgctgaaca gcgcgctgca taagtctctg ttgctcatct gcaaccagag ccattttctt
     7141 ctcaggtggc tcggtggatt ccagtttttc tcgaagttgt tcgagtgcag ctgcttgggc
     7201 agcgacagcg gcctgtgcgg cagcggcctg tactgctgct gcttgtgcgg ccacaacagg
     7261 atggccggcc aaggacacag gcaacctacc gggcacggta attgggattg ctgagtcttc
     7321 ttccttcttg attttaggtg ctggggtaat tgatgagcca ttagtagaag cagccagtcc
     7381 gagtggagtg acagggagtt ttggactagg gagcattgaa tgggctccac tggggctata
     7441 agcgaagagg cttcccccga agctctggcg cctgccttct ctcctattac tatcaatagc
     7501 agcttggagt tcattaggag aagaaagacc cctgcgttcg cattcatagg gatagagata
     7561 tttcatgtac tgagttctca gtgtgaatgc agctgaggtt atactggtag gcaaattcaa
     7621 tccctttgta atttctctcc aaagtttctt gttgattact tcgacgagcc ctcccttctc
     7681 agttacaagg acatacagca taaacaggtc cagtacttgc ttagccatta ttggaatcct
     7741 gtttacaggg gtgcctcgct tctgcataaa ggagaagaga tcatccagaa actctttccg
     7801 ttttggatct gcgtcgagct cataaagctg tttaaattgt tcctcgaaag tccagtcgcc
     7861 atggtcgggt ggaggcatct gtgaggccat gtgacttggg tgggcggggc caggacccaa
     7921 tctttcgggt ccagagagca tgcggggccc tccgtcaccc cgaaatgcct gtgctggagg
     7981 ggcctttcgg gtgaagagtc cagctgtccc cagggaggcg gattcgggag gacccaaacc
     8041 ctcctcctct tcttcttcct cctcaaaatc gtcctcctcc tcttcttcct cttcctcctc
     8101 tcccaattct tccaactcct gctcttccca tttagacttt gtatcatcat cgtctgaccc
     8161 aacatccatc aaatgccgat ctccctcggc atgaccagca ccctccaaca cgctgccatg
     8221 gagtgctgga ctagaaagag taccctccct ggcggtgtct tcatcaccgg agattggggg
     8281 cccgtcttca gagcctccgg gactagaagg gtgacccaat ccagcagcag cagcgcgcat
     8341 ggcggccaag gctgccatct gagtccgatg catccgggca ttttctggct ctctgtcctc
     8401 gtcagtcatg gttgttcgag cgcgaactcc tgtaggttca ggagggggag gaggaggggc
     8461 ttggcgagct tccagttctt gtcgtgctct ctgttgccgc tgaatgagag tttccatgac
     8521 ggcttgcaac ttcatggtgg cccggtggat ccgtcgacgg tatcgataag cttgattcga
     8581 ccggccgact cagtctgtcg gaggactggc gcgccgagtg aggggttgtg agctctttta
     8641 tagagctcgg gaagcagaag cgcgcgaaca gaagcgagaa gcaggctgat tggttaattc
     8701 aaataaggcg cagggtcatt tcaggtcctt gggggagcct ggaaacatct gatgggtctt
     8761 aagaaactgc tgagggttgg gccatatctg gggaccatct gttcttggcc tcgggccggg
     8821 gccgaaactg cggtgaccat ctgttcttgg ccccgggccg gggccgaaac tgctcaccgc
     8881 agatatcctg tttggcccaa cgttagctat tttcatgtac ccgcccttga tctgaacttc
     8941 tctattcttg gtttggtatt tttccatgcc ttgcaaaatg gcgttactgc agctagctcg
     9001 agccggcgcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     9061 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     9121 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     9181 atttgtctca agatctagaa ttgtcccctc ggggttggga ggtgggtctg aaacgataat
     9241 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     9301 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     9361 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     9421 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     9481 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     9541 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     9601 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     9661 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     9721 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     9781 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     9841 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     9901 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     9961 tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt
    10021 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
    10081 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
    10141 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
    10201 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
    10261 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
    10321 cttaatactg acgctctcgc a
//
