LOCUS       sequence-338327-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmids for gene knockout were constructed based on suicide plasmid
            pHFX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J
  TITLE     Optimising PHBV biopolymer production in haloarchaea via 
            CRISPRi-mediated redirection of carbon flux.
  JOURNAL   Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z.
  PUBMED    34433872
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-08-25"; volume: "4(1):1007. doi"; pages: " 
            10.1038/s42003-021-02541-z"
COMMENT     Sequence Label: pHFX-cas3UD
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctcgttgac ccggcgtacc gtctctatgt ctggatgagt gattcccatt ggttcgagac
       61 gctccacgag acgctcgacg aaggcaaatc acactacgtt ccgagtcttg gtctttctga
      121 gtacctcgca gagatcacgt accacggacg cttcgaggtt gagtctggac cgactgacac
      181 tgcggtggca gtcgactctg ccgtcccgaa cgcggtcgat catgtcgttc cagacgctga
      241 atcccggtgt caaatcgaag agtcgcccgc gttcatgaca gttgacggag gcggacgaac
      301 aacgactgac ttcaccagct acacgtacaa tccagacgca ggaccggttc gggtacgaaa
      361 tccggacacg gctatcgtcg acggaaacac ggtgatgttc gtttgagatg accgacgtcg
      421 atccagttca acgactcctg cgaactgcga gagatgacgc gcgagacgaa tcgtttcggg
      481 taaccggcgt catgatgcag tattacgtcg tctgtaagcg cgaactctgg tttcacagcc
      541 gtcatatcga gattgaccgc gggaactccg ctattgtccg aggaacccat atcgacgaaa
      601 ccgcgtatag cgacaaacgg cgacatgtct cgattgacag tacaattgcg attgacgtat
      661 tggatgatgg tcgtgtgatg gaggtcaaac cttcctcggc actcgtcgaa cctgcgaaac
      721 tccagttact ctactactta tggtatctga agcacgttgt tggtgtcgag aaatctggcg
      781 tacttgcaca cccgacagag cgcaagcgag aagacgtaga attgactg
//
