LOCUS       sequence-338330-       12165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of Luciferase cDNA with dTomato as 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: 
            SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..12165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3375..5024
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3441..3459)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    join(5043..5617,5618..5620,5621..5629)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5210..5227)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5437..5456
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5636..6337
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5769..5787)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6037..6056
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6231..6254
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6362..6861
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6397..6420)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6794..6813
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6868..6877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6874..6876,6877..6879,6880..7590)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7603..7659
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7660..8406
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8433..9021
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8486..8506)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8904..8915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9093..9326
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9404..9525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9441..9460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9495..9514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9565..9700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9720..9739)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9721..9739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9749..9766)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9749..9765)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9758..9780)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9907..10362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9994..10013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10204..10225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10388..10492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10493..10561,10562..11353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10711..10730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11524..12112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12013..12032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
     3061 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag
     3121 agaacgtatr wccagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagac cggtatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     3421 ctctagagga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     3481 ttcctggaac aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat
     3541 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     3601 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     3661 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     3721 acagtatgaa catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa
     3781 ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa
     3841 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     3901 ttaatgaata cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa
     3961 tgaattcctc tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct
     4021 gcgtcagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcgtt ccggatactg
     4081 cgattttaag tgttgtccca ttccatcacg gttttggaat gtttactaca ctcggatatt
     4141 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat
     4201 cccttcagga ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg
     4261 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg
     4321 gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga
     4381 tacgacaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     4441 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     4501 tggataccgg gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta
     4561 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     4621 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg
     4681 accgcttgaa gtctttaatt aaatacaaag gatatcaggt ggcccccgct gaattggaat
     4741 cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg
     4801 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     4861 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     4921 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     4981 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgctcgag tgaacgcgtt
     5041 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5101 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5161 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5221 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5281 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5341 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5401 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5461 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5521 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5581 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaaccg gatccatggt
     5641 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     5701 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5761 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     5821 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     5881 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     5941 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     6001 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     6061 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     6121 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     6181 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     6241 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     6301 ccacctgttc ctgtacggca tggacgagct gtacaagtga gcgatatcgc gtaattctac
     6361 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     6421 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggaagg
     6481 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     6541 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     6601 cgtgtcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     6661 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     6721 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     6781 accggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     6841 tcctcatctc cgggcctttc ggctagcgcc accatgggat ctaagggcga ggaactgttc
     6901 accggcgtgg tgcccatcct ggtggaactg gatggcgacg tgaacggcca caagttctct
     6961 gtgcggggag agggcgaagg cgacgccaca aatggcaagc tgaccctgaa gttcatctgc
     7021 accaccggca agctgcccgt gccttggcct accctcgtga ccacactgac ctacggcgtg
     7081 cagtgcttca gcagataccc cgaccatatg aagcggcacg acttcttcaa gagcgccatg
     7141 cccgagggct acgtgcagga acggaccatc agcttcaagg acgacggcac ctacaagacc
     7201 agagccgaag tgaagttcga gggcgacacc ctcgtgaacc ggatcgagct gaagggcatc
     7261 gatttcaaag aggacggcaa catcctgggc cacaagctgg agtacaactt caacagccac
     7321 aatgtgtata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgg
     7381 cacaacgtgg aagatggcag cgtgcagctg gccgaccact accagcagaa cacccccatc
     7441 ggagatggcc ccgtgctgct gcccgacaac cactacctga gcacccagag cgtgctgagc
     7501 aaggacccca acgagaagcg ggaccacatg gtgctgctgg aattcgtgac cgccgctggc
     7561 atcacccacg gcatggacga gctgtataag ggaggaagcg gcgccaccaa tttcagcctg
     7621 ctgaaacagg ccggcgacgt ggaagagaac cctggcccta tgagccggct ggacaagagc
     7681 aaagtgatca acagcgccct ggaactgctg aacggcgtgg gcatcgaggg cctgaccacc
     7741 cggaagctgg cccagaaact gggcgtggaa cagcccaccc tgtactggca cgtgaagaac
     7801 aagcgggccc tgctggacgc cctgcccatc gagatgctgg accggcacca cacccacagc
     7861 tgccctctgg aaggcgagag ctggcaggac ttcctgcgga acaacgccaa gagctacaga
     7921 tgcgccctgc tgagccaccg ggacggcgcc aaagtgcacc tgggcaccag acccaccgag
     7981 aagcagtacg agacactgga aaaccagctg gccttcctgt gccagcaggg cttcagcctg
     8041 gaaaacgccc tgtacgccct gagcgccgtg ggccacttca ccctgggctg cgtgctggaa
     8101 gaacaggaac accaggtcgc caaagaggaa agagagacac ccaccaccga cagcatgccc
     8161 cccctgctga agcaggccat cgagctgttc gacagacagg gcgccgagcc cgccttcctg
     8221 ttcggcctgg aactgatcat ctgcggcctc gagaagcagc tgaagtgcga gagcggcgga
     8281 cccaccgacg ccctggacga cttcgacctg gacatgctgc ccgccgatgc cctggatgat
     8341 tttgatctgg atatgctccc tgctgacgct ctcgatgact tcgatctcga tatgctgcca
     8401 ggctaatgta caagtgatcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     8461 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8521 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8581 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8641 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8701 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8761 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8821 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8881 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8941 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     9001 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     9061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9121 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9241 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9301 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     9361 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     9421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     9541 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9601 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9661 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     9721 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     9781 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9841 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9901 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     9961 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10021 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10081 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10141 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10201 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10261 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    10321 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
    10381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    10501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    10741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    11041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    11221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    11281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    11341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    11401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    11701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgc
//
