LOCUS       sequence-338339-       14586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble human PACE/furin protease expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Kovnir SV, Orlova NA, Shakhparonov MI, Skryabin KG, Gabibov AG, 
            Vorobiev II
  TITLE     A Highly Productive CHO Cell Line Secreting Human Blood Clotting 
            Factor IX.
  JOURNAL   Acta Naturae. 2018 Jan-Mar;10(1):51-65.
  PUBMED    29713519
REFERENCE   2  (bases 1 to 14586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Naturae. 2018 Jan-Mar"; volume: "10(1)"; pages: "51-65"
COMMENT     Sequence Label: p1.2-Hyg-Fur
FEATURES             Location/Qualifiers
     source          1..14586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..55)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(209..797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(289..308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(968..1759,1760..1828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1591..1610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1829..1933)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      6471..6480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7774..7783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12914..12934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12919..13248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13099..13234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13161..13180
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13297..14322
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             13297..14322
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    14452..14573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14489..14508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14543..14562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccgtcgacct ctaggcgcgc catggctctt ccgcttcctc gctcactgac tcgctgcgct
       61 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1921 taggggttcc gcgcacattt ccccgaaaag tgccacctgc atctcatcac ggtcacgcat
     1981 ggctgcgccc gcgcagcgcc ccccgacccc cccccgatcc ccggaacgcc agccgccatc
     2041 caccgcccgc agcccccgga gcccgcggac cccgaccccc cgccgctcgc ccgccgttgc
     2101 gccgctctct gcgggggggc tagggggccg cggggggaag gccacgcccc ctccactttt
     2161 tccaggaatg cgcggcatga cccgcccccc ccatgacccg ccaacacccc cccgatgccc
     2221 cccgggggtc tttcctgggg ggcctttgtc agggttgcct gtgtcaccca cgggcccctt
     2281 ggccacgctt gcctgggtca ccccggagcc ccgggtcacg tttgccgggg tcagccctgg
     2341 ggcgcttctt cacgctccat tgccatgcag aattcactag tgattagaat cccacgttgt
     2401 gcatagaaac agatgcaggc aaaacatcca cacatataaa acaaaaaatt aaaaccaata
     2461 aaactcctaa acttttggtc tttcttgaat cttcaatccc tcaggttatg aaataatcat
     2521 ttatgcagtc aaaaatttgc cattcttgtt gccaggtgtg gtgatgattc ggggaagcag
     2581 aagcaggcag atctctgtga atgaggccag cctggtctac aaagtgagtc ccaggacagt
     2641 caggcctgtt acacagagaa accttgaaaa aaaaaagata atatgtactg ttgtattacc
     2701 ccaatatata aggctaaacc attagaagca caacactgtt aagtacggaa aataatatct
     2761 agtgtggtac agttactact actataatac actaatatag ctgtgggaaa ctagttccaa
     2821 agatgaatta ctaaccagtg tttccaagga aataaatgaa agcagagaga ttagttctat
     2881 tgctagtgtt tcattttcgt atatttctta caatttctct tgttacaaat aggcactagg
     2941 gtatcaagat aattttaacg actggctgag aaccctagaa aatctctgtg aaaaagggat
     3001 ttgtgaaatg agagagggta atgtggccat tatagaaaag gcttttgtgt gccttgcatg
     3061 catagaccct gtgtttgatc tcttaacacc ctccttgacc agaaaaagct tctgtggata
     3121 gaaaatgatt agttatatat acttttaggg aaacgtagtt ctggattctt tggttacaat
     3181 taacagaatt aagtgcaaac aaagccagaa acctcctgat aaatgagaaa acctgcttgt
     3241 agaaggttgt aaggctctgt aatataggaa ttaggagaaa agaaacctgt gtggtggggc
     3301 acgtctgtaa tcccagcatt gggaagtaga ggtagaagat tagaaatcaa aggccagcct
     3361 cagcaacaca gtgagtttga ggccaccctg aactacatca ggttctgtct cctttctttt
     3421 tttttttttt cttttctttt tttggtttct ctgtgtagtt ttggagccta tcctggcact
     3481 agctctgaag agcaggctgg cctcgaactc agagatcagc cagcctctgc tgggattaaa
     3541 ggtatgcacc accaacgccc caggttttgt ctcaaacaaa caaaaataac atcaggaggt
     3601 ggtgagaggg ctcagtggtc acaggcattc tctgcaaagc ctgactctga gttggatcct
     3661 ttagagctac atggttgagg gaagagaact gactcctgga aggtgtcctc tggtccccac
     3721 acatagctat acacagcatg tgcattcaca cacactaaat aatgctattt ttaaaaaaat
     3781 taaaaacaac aacagtttgg gttgtgaaaa ctagaactag ataataggta agaatcaagt
     3841 atcatgtaaa tttgctttca actcatccca aaatttgttt tatatttcag tttttttcct
     3901 tcctagcttg actgtggagt cttgtccgga agcaaatagt tcctttgcag atcccacatg
     3961 tggacaccgg acagtaggtc ctcaaatgct ccttattagg ttggttcaat aatatcaatt
     4021 gtttgttact aggcagtgat gttgtacatc tggaggagat ctcttgagcc cataatcagg
     4081 ttattaggaa taaatactct aaggctaaaa atgtagctta gtgataagag tgcttgcctg
     4141 gtgtgctgag accctcggtt ccatctccac aaccccatat tccattacaa aatacctttt
     4201 caccgtccct agcattaaga aacaaaacaa caaagaagtt tttctttctt ctgagatcct
     4261 gcccggagag gcatttaaaa ctggccaggg ccaaaaaaaa aaaaaaaaga aaaaaaagaa
     4321 aagaaaacag gctagggccg gcatggtggc gcacgccttt aatcccagca cgcaggaggc
     4381 agaggcaggg cggatctctg tgagtttgag gtcagcctgg tctacctagt gagtttcagg
     4441 gcacccaggg ctaaagagac tgtctcaaaa acaaaacagc cacacaatca gaaccacagc
     4501 aaaacgcagt tatgatcctt ggaactgtag gaatgacaag catttaaata ataggacgag
     4561 ccatttttga gaagctctga tttcacaagt gtcagggatg ggctctgggc gagtaagatt
     4621 gctaatgctg gcctctaaat gagaccacgt ggagttgatt agattctttt catgttcctc
     4681 gtgctctatc aaataactgt acccaaatac acacacacac acacacacac aatgcgcgca
     4741 cacacaaaat ccttttttag cttaagaagc ccagaatcag aagtaaagct aactgtggga
     4801 cttaagtatt attctgaacg gaactcccag ggcgtgaagc gcgcttcagg cttccagaga
     4861 agcagctggc gctggatgga atgaaccaag aggccagcac aggggcagat ccgtcgagct
     4921 ctcggccacc gagctgagcc cttaggttct ggggctggga agggtcccta ggattgtgca
     4981 cctctcccgc gggggacaag cagggggatg gcggggctga cgtcgggagg tggcctccac
     5041 gggaagggac acccggatct cgacacagcc ttggcagtgg agtcaggaag ggtagggaca
     5101 gattctggac gccctcttgg ccagctcctc accgccccac ccccgactgg agccgagagt
     5161 aattcataca aaaggaggga tcgccttcgc ccctgggaat cccagggacc gtcgctaaat
     5221 tctggccggc ctcccagccc ggaaccgctg tgcccgccca gcgcggcggg aggagcctgc
     5281 gcctagggcg gatcgcgggt cggcgggaga gcacaagccc acagtccccg gcggtggggg
     5341 aggggcgcgc tgagcggggg cccgggagcc agcgcggggc aaactgggaa agtggcgtcg
     5401 tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtataaaagt gcggtagtcg
     5461 cgttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg
     5521 gcctccgcgg gcccgggctc cctcctttga gcggggtcgg gccgccgtgc gggtgtcgtc
     5581 ggccgggctt ctctgcgagc gttcccgccc tggatggcgg gctgtgcggg agggcgaggg
     5641 ggggaggcct ggcggcggcc ccggagcctc gcctcgtgtc gggcgtgagg cctagcgtgg
     5701 cttccgcccc gccgcgtgcc accgcggccg cgctttgctg tctgcccggc tgccctcgat
     5761 tgcctgcccg cggcccgggc caacaaaggg agggcgtgga gctggctggt agggagcccc
     5821 gtagtccgca tgtcgggcag ggagagcggc agcagtcggg ggggggaccg ggcccgcccg
     5881 tcccgcagca catgtccaac gccgcctgga cgggtagcgg cctgtgtcct gataaggcgg
     5941 ccgggcggtg ggttttagat gccgggttca ggtggccccg ggtcccggcc cggtctggcc
     6001 agtaccccgt agtggcttag ctccgaggag ggcgaggccc gcccgcccgg caccagttgc
     6061 gtgcgcggaa agatggccgc tcccgggccc tgtagcaagg agctcaaaat ggaggacgcg
     6121 gcagcccggc ggagcggggc gggtgagtca cccacacaaa ggaagagggc cttgcccctc
     6181 gccggccgct gcttcctgtg accccgtggt gtaccggccg cacttcagtc accccgggcg
     6241 ctctttcgga gcaccgctgg cctccgctgg gggaggggat ctgtctaatg gcgttggagt
     6301 ttgctcacat ttggtgggtg gagactgtag ccaggccagc ctggccatga aagtaattct
     6361 tggaatttgc ccattttgag tttggagcga agctgattga caaagctgct tagccgttca
     6421 aaggtattct tcgaactttt tttttaaggt gttgtgaaaa cctcgaggcc gccaccatgg
     6481 agctgaggcc ctggttgcta tgggtggtag cagcaacagg aaccttggtc ctgctagcag
     6541 ctgatgctca gggccagaag gtcttcacca acacgtgggc tgtgcgcatc cctggaggcc
     6601 cagcggtggc caacagtgtg gcacggaagc atgggttcct caacctgggc cagatcttcg
     6661 gggactatta ccacttctgg catcgaggag tgacgaagcg gtccctgtcg cctcaccgcc
     6721 cgcggcacag ccggctccag agggagcctc aagtacagtg gctggaacag caggtggcaa
     6781 agcgacggac taaacgggac gtgtaccagg agcccacaga ccccaagttt cctcagcagt
     6841 ggtacctgtc tggtgtcact cagcgggacc tgaatgtgaa ggcggcctgg gcgcagggct
     6901 acacagggca cggcattgtg gtctccattc tggacgatgg catcgagaag aaccacccgg
     6961 acttggcagg caattatgat cctggggcca gttttgatgt caatgaccag gaccctgacc
     7021 cccagcctcg gtacacacag atgaatgaca acaggcacgg cacacggtgt gcgggggaag
     7081 tggctgcggt ggccaacaac ggtgtctgtg gtgtaggtgt ggcctacaac gcccgcattg
     7141 gaggggtgcg catgctggat ggcgaggtga cagatgcagt ggaggcacgc tcgctgggcc
     7201 tgaaccccaa ccacatccac atctacagtg ccagctgggg ccccgaggat gacggcaaga
     7261 cagtggatgg gccagcccgc ctcgccgagg aggccttctt ccgtggggtt agccagggcc
     7321 gaggggggct gggctccatc tttgtctggg cctcggggaa cgggggccgg gaacatgaca
     7381 gctgcaactg cgacggctac accaacagta tctacacgct gtccatcagc agcgccacgc
     7441 agtttggcaa cgtgccgtgg tacagcgagg cctgctcgtc cacactggcc acgacctaca
     7501 gcagtggcaa ccagaatgag aagcagatcg tgacgactga cttgcggcag aagtgcacgg
     7561 agtctcacac gggcacctca gcctctgccc ccttagcagc cggcatcatt gctctcaccc
     7621 tggaggccaa taagaacctc acatggcggg acatgcaaca cctggtggta cagacctcga
     7681 agccagccca cctcaatgcc aacgactggg ccaccaatgg tgtgggccgg aaagtgagcc
     7741 actcatatgg ctacgggctt ttggacgcag gtgccatggt ggccctggcc cagaattgga
     7801 ccacagtggc cccccagcgg aagtgcatca tcgacatcct caccgagccc aaagacatcg
     7861 ggaaacggct cgaggtgcgg aagaccgtga ccgcgtgcct gggcgagccc aaccacatca
     7921 ctcggctgga gcacgctcag gcgcggctca ccctgtccta taatcgccgt ggcgacctgg
     7981 ccatccacct ggtcagcccc atgggcaccc gctccaccct gctggcagcc aggccacatg
     8041 actactccgc agatgggttt aatgactggg ccttcatgac aactcattcc tgggatgagg
     8101 atccctctgg cgagtgggtc ctagagattg aaaacaccag cgaagccaac aactatggga
     8161 cgctgaccaa gttcaccctc gtactctatg gcaccgcccc tgaggggctg cccgtacctc
     8221 cagaaagcag tggctgcaag accctcacgt ccagtcaggc ctgtgtggtg tgcgaggaag
     8281 gcttctccct gcaccagaag agctgtgtcc agcactgccc tccaggcttc gccccccaag
     8341 tcctcgatac gcactatagc accgagaatg acgtggagac catccgggcc agcgtctgcg
     8401 ccccctgcca cgcctcatgt gccacatgcc aggggccggc cctgacagac tgcctcagct
     8461 gccccagcca cgcctccttg gaccctgtgg agcagacttg ctcccggcaa agccagagca
     8521 gccgagagtc cccgccacag cagcagccac ctcggctgcc cccggaggtg gaggcggggc
     8581 aacggctgcg ggcagggctg ctgccctcac acctgcctga gtgatagtct agcatattac
     8641 ccctaacacc tgccacccca gtcttaatca gtggtggaag aacggtctca gaactgtttg
     8701 tctcaattgg ccatttaagt ttaatagtga aagactggtt aatgataaca atgcatcgga
     8761 aaaccttcag gaggaaagga gaatgttttg tggaacattt ttgtgtgtgt ggcagtttta
     8821 agttattagt tttcaaaatc agtacttttt aatggaaaca acttgaccaa aaatctgtca
     8881 cagaattttg agacccatta aaatacaagt ttaatgagaa gtctgtctct gttaatgctg
     8941 aagtcattac taagtgctta gcttagcaag gtatgtggat gcccatttgt gttccaaggg
     9001 attggactgt tcatcaggac ccagagctga gtttcaaggg ctcaagagat ggcttattac
     9061 ctgtgggtgt cttgaaggtt ctggttggga caaattagga atgtttttgg cagacatggt
     9121 gactaccctt catctgggtg agttcagttg atttgtcttg agcctttggg gtttacacaa
     9181 gtaaatgaca tcatacagtt agtgtattgt tagtgaatat taatatatga ggcaggcttt
     9241 gctctagcaa ttttagaact agttttcagg aaagggttca tcttgtgcat tggatgtctg
     9301 attctatcac ttagagttta aactgaaagt gctcaagagg ttttatttag gctgggatat
     9361 aaataagcct ttctgtagct tgtaatggta tcaggaattt aaaaggccat ctggggcaca
     9421 aagattaagc agaaaaggta gaaggtgaga ttgggggact ttgagtactt cacacacttt
     9481 aatgtgtggg tgctttagtg catatagtac aactgccaga taagggcatc cacatctgat
     9541 tgtttggaag gcaccttgtg gtttctggga attcagaatt gggagaaaaa gtgctcccaa
     9601 ccgctgaagc ccttggtaat ctgcagggtg tttatttagc aggagataag gacaaaaagt
     9661 tatagtgtgg agttggttga gttggtagat gtcattacaa caggtggtct taaattgggt
     9721 taggagtcac tttgaaatac ctgggccata agcaaagtgg cattttcacc tttcaggaga
     9781 aactggtaca cttatccatt ctatagtgca tgcttgttca attgggctga tgactaaacc
     9841 ggtgactaaa ggtttgtcag tataaatgga tgggttgtag gcagacggtg aggaattact
     9901 atacctgcaa ggagtcattg cctgatctgc ctggaaaggg gcaggattga gtctcagaag
     9961 gtgtacacca taggatatgg aaaaatttgt cacgcctagc attcaactta gtggtgtagc
    10021 gccacctact ggcactttaa aagcttagca tagaggagca tgtgtgttag gagctcggat
    10081 gggatccagg gcctcaaggt ttgcatgtaa ataaaagccc tttaccaaat taactacata
    10141 ccagcataca tcagtccttt agtgttgaaa aacagaagtg aaagctaata tatatagtgc
    10201 ttgctttatt taagtctagc tgattacgtg tttggttgcc agtgtgacta gtctggagtt
    10261 gaatttgtcc tcagacacgt aaaatggaat ttgggattca caacactcta gtatgaggga
    10321 cctaatggcc tgtaccaggc acaaacgtgt ctataaacta cacaaaacga aggaatttac
    10381 aggaattagg aaggtattct taacattaaa acattatggg cattttaaaa aaagctttga
    10441 caggatttct ttgtcatggc tgtcctggag ctagttgtgt agaccaggct gggctgaaat
    10501 cttgtctgcc tgcctggctt ggacactttt ttattatgta tacaacattc tgcttccatg
    10561 tatatctgca cattagaaga cggcaccaga tctcctaatg gatggttgtg agccaccatg
    10621 tggttgctgg gaattgaact caggacctct ggaagagcag tgctcttaac ctctgagcca
    10681 tctccagccc cagcttgggc acatttttaa tggctgggaa atcaaacccc ctaggccttc
    10741 tgtcagtaat gaagggcttt tggctaccga gagtaggatt taaggttatt cggagctgca
    10801 ggtctgcctc agtgcaggtt tgggagtcca gcatcttaga aaatgcagtg aagccaagct
    10861 gagctatatt ttgtttaaaa aaaaaataag tgggtaaagt gctgctgagc ctgatgacca
    10921 agctgggaca caagtagaag aacataggcc aatgctctat attaaaagca tgggtcattt
    10981 ttaatgctct tgagaaggct atgcctacac tactctcagc caccgcagcg tgtttaaatt
    11041 aaactagttt ggaaattttc tttgggggta agctatttaa cctagtgcct tggcaggtat
    11101 actactgaac tctcctcctc attccttttt gttttttaag aatttcagtc aggctcaggc
    11161 agcccttaaa cttgtgatta agcctgagaa cagttacgat tatgagccta ttagtatacc
    11221 gatcaatatg tgaatttttt tgggatgggg gtcaggcctc cctgcctccc aaatactggg
    11281 actaaaggct gcaccaccac aacctggctc ttgaaatact tttctacatt ttttgggggg
    11341 catgggtggg agagcagggt ttctctgtat tagccctggc tctcctggaa ctctgtagac
    11401 caggctattc ttgagctcag attagcctgt ctctgcctcc taaattctgg gattaaaggt
    11461 gtgtgctact gctgcctggc tacaaagaca tttttttttt tcttaaattt aaaaacaaaa
    11521 gtggttcttt tagaagggtg gttggtgttg gcacatactc caagcactca ggttttgagt
    11581 ttgtcccagg aatgaagact gcattactgc cgcccctccc tggtaagggc tacacagaga
    11641 aatcctattt ggagcctgtc ctggtaactc gctctgtaga ccaggctggc ctcgaactca
    11701 agagaaccac ctgcctctga atgctggtat taagggcagg caccaccaac acccagccta
    11761 aaaaatgtct tttttttaaa gatttttttt ttttttttac agaataaaca ttctgtttac
    11821 aatattctgc ttctatgtat atctgcacac tagaagaggg cacccgatct cataatggat
    11881 ggttgtgagc caccaagtgg ttgctgggaa ttgaactcag aacctctgga agagcagtca
    11941 gtgctcttaa cctctgagcc atctctccag cccctaaaaa tggctcttga gatagggtct
    12001 caagtagttt gagactgagt tggctatata aacaaggctg gcacatagca ccatgtacag
    12061 ctgggtttag tttacatggg gtgtttttgt ctctggaggc aggaggatca tttgagcata
    12121 gggagttaat agtgaggtca tgttttatct actcttctga attgagaact aagctgatgc
    12181 aaagcaagtt tgactgaaga agtccagttt atgagaacaa gggtggaaac taatgtgtca
    12241 aagatggcct tgcatgtgtt ttagatgatg acccagtcac ttgggaatta ctggatgtgt
    12301 aagacctata tcttgacagg agtgaacagt gtcttatagg tcctatatga aagaaatgag
    12361 acatacccat tttgtttccc ctaagaattc acttttccta acctggttca tgctatttag
    12421 gttattttac ttgcaaatcc tagggtgctc ccttacccag tattgcttat gtggcaccaa
    12481 agtcactcac tcccatgatt tgcaagtctc tgggaacttc catgacaacc tagaatagca
    12541 actcaaatac attttctcag taccaatttt gaagaaaaaa tattttgcaa aatagctgta
    12601 tggatgggta ctaaatagtg aggttatctc cagaaggcct atgaagaatt aaggttgagt
    12661 tcagttgagt tcagcagcaa gtttaaggtt catccatttt tgtacagtgt tttcctatta
    12721 cggtaagtgt tttgcctgca ggaatatctg taccacatgc ttgcctggta cctatatcgg
    12781 ccagaagagg gctttggatc ctctggactt gaattacaga tgggtattag ccaccattta
    12841 ggtgctggga attgaaacca agtcctctgg aagaacagca agtgactcga cgtccggagc
    12901 tctggcgcgc cgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    12961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    13021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    13081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    13141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    13201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    13261 gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga actcaccgcg
    13321 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
    13381 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
    13441 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
    13501 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
    13561 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
    13621 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
    13681 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
    13741 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
    13801 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
    13861 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
    13921 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
    13981 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
    14041 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
    14101 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
    14161 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
    14221 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
    14281 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct acgagatttc
    14341 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    14401 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    14461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    14521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    14581 tgtata
//
