LOCUS       sequence-338342-        2042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S. Typhimurium stm2585 (sarA/steE) flanked with endogenous 
            regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, 
            Brennan RG, Ko DC
  TITLE     The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor 
            Signaling to Activate STAT3.
  JOURNAL   Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 
            10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.
  PUBMED    31901521
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.11.012"; journalName: "Cell Host Microbe"; 
            date: "2020-01-8- 8"; volume: "27"; issue: "1"; pages: "129-139.e4"
COMMENT     Sequence Label: pWSK129-stm2585
FEATURES             Location/Qualifiers
     source          1..2042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     98..118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        100..118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1532..1551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1533..1551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1561..1578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1561..1577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1570..1592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1806..1825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2016..2037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagac tacatgccaa ctttacttag
      181 tatcatattt tcttgctaca tatctgtttg gcaagggtat gaataaaaaa caagattaat
      241 ttaattgata tcgcaacaga taagcacctg catttatctt taaacttttt tatggccaac
      301 cagatttttc accatctgcg cacgtttatt cgggcatgcg ttgaaaacct gcaaaaaaag
      361 aaaaatatcg atgcttatta ttttttcttt aattaaattt tcgctcaaca aacttaatta
      421 ttaatccaat gacagtgaag tgtgaactat gctgagcata aacgacatca atagcaaggg
      481 caagggcaag ggcaagggca agggcaaggg caagggcaag ggcaagggca atctgagtat
      541 ttacaggtga aattatgaaa aatagtatat ttttaccatt gcttctggtg ctgtcggcaa
      601 cgaccttttc ggcattggtg atggctgcca gtcattctcc ccaccagata atacaaaaca
      661 tttttccagt ggctggccgc cagatatcct gaagcaactg aattggtctc aggtttgtgg
      721 ttgttagtat actgaatatt acttcaggat ggcatgtttt attgacacca gcctgatttt
      781 tcacaacgtt atattacccg tttacagata attcccagct aataaatcag gaagttccag
      841 atgtaacaga agttatgagg tgtattaatt gataggtcta acaggcttga attacttttc
      901 tttaaaaact actgcatgta aaagggtctc ctcttgttgt gatgagattc gtatatattt
      961 atatcttagt gatttatgga tcatacttgt ggttttcctt aggaggtaac atgtttacaa
     1021 ttaatagtac taacagggtg gcgagcacaa tcgctccata tgcatgtgta agcgatgtta
     1081 atttagagga caaggctaca tttttagatg aacatacctc aatacatgcg aatgattctt
     1141 ctttgcaatg ctttgtctta aatgatcagc atgttccgca gaatacatta gctacagatg
     1201 ttgaaggcta caatagagga ttacaggaac gtataagcct cgagtaccag cccctggaaa
     1261 gcattgtttt tctactcggc acccctgcag ttttagagac taaagagtct ttatcattac
     1321 cagtttcccc ggatgcttta acccaaaaac tattaagcat tagtagcaat gatgaatgta
     1381 aattatcagg tagcaccagt tgcactaccc cagccagtca taacccaccg tccggttata
     1441 tcgctcaata caggcattct gcagaggttt tcccggatga ataaaacaaa gcgttataaa
     1501 tagctgcgcc gagggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     1561 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1621 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     1681 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aa
//
