LOCUS       sequence-338346-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of miR-ctrl with dTomato as 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..78
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(139..141,142..144,145..855)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             868..924
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             925..1671
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1698..2286
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1751..1771)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2169..2180)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2358..2591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2669..2790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2706..2725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2760..2779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2830..2965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2985..3004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2986..3004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3014..3031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3014..3030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3023..3045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3172..3627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3259..3278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3469..3490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3653..3757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3758..3826,3827..4618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3976..3995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4789..5377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5278..5297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5531..5548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5665..5686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5701..5706,5707..5724,5725..5731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5739..5755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5744..5766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5763..5779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5763..5779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5798..5818
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5800..5818
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        5863..6242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        6299..6490
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6322..6342)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6509..6735
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6736..6916
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6963..7088
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7581..7814
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7999..8043
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8256..8373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8419..8797
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    8427..8445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8463..8481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8499..8517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8535..8553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8571..8589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8607..8625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8643..8661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8706..8730
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8804..8813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8810..9511
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(8943..8961)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9211..9230
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9405..9428
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           9536..9657
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9685..9699
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9727..9853
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        join(9876..10249,1..126)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9911..9934)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtccaccgg aggcccggca
       61 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
      121 ctttcggcta gcgccaccat gggatctaag ggcgaggaac tgttcaccgg cgtggtgccc
      181 atcctggtgg aactggatgg cgacgtgaac ggccacaagt tctctgtgcg gggagagggc
      241 gaaggcgacg ccacaaatgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      301 cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg gcgtgcagtg cttcagcaga
      361 taccccgacc atatgaagcg gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
      421 caggaacgga ccatcagctt caaggacgac ggcacctaca agaccagagc cgaagtgaag
      481 ttcgagggcg acaccctcgt gaaccggatc gagctgaagg gcatcgattt caaagaggac
      541 ggcaacatcc tgggccacaa gctggagtac aacttcaaca gccacaatgt gtatatcacc
      601 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa cgtggaagat
      661 ggcagcgtgc agctggccga ccactaccag cagaacaccc ccatcggaga tggccccgtg
      721 ctgctgcccg acaaccacta cctgagcacc cagagcgtgc tgagcaagga ccccaacgag
      781 aagcgggacc acatggtgct gctggaattc gtgaccgccg ctggcatcac ccacggcatg
      841 gacgagctgt ataagggagg aagcggcgcc accaatttca gcctgctgaa acaggccggc
      901 gacgtggaag agaaccctgg ccctatgagc cggctggaca agagcaaagt gatcaacagc
      961 gccctggaac tgctgaacgg cgtgggcatc gagggcctga ccacccggaa gctggcccag
     1021 aaactgggcg tggaacagcc caccctgtac tggcacgtga agaacaagcg ggccctgctg
     1081 gacgccctgc ccatcgagat gctggaccgg caccacaccc acagctgccc tctggaaggc
     1141 gagagctggc aggacttcct gcggaacaac gccaagagct acagatgcgc cctgctgagc
     1201 caccgggacg gcgccaaagt gcacctgggc accagaccca ccgagaagca gtacgagaca
     1261 ctggaaaacc agctggcctt cctgtgccag cagggcttca gcctggaaaa cgccctgtac
     1321 gccctgagcg ccgtgggcca cttcaccctg ggctgcgtgc tggaagaaca ggaacaccag
     1381 gtcgccaaag aggaaagaga gacacccacc accgacagca tgccccccct gctgaagcag
     1441 gccatcgagc tgttcgacag acagggcgcc gagcccgcct tcctgttcgg cctggaactg
     1501 atcatctgcg gcctcgagaa gcagctgaag tgcgagagcg gcggacccac cgacgccctg
     1561 gacgacttcg acctggacat gctgcccgcc gatgccctgg atgattttga tctggatatg
     1621 ctccctgctg acgctctcga tgacttcgat ctcgatatgc tgccaggcta atgtacaagt
     1681 gatcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1741 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1801 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     1861 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     1921 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     1981 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2041 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2101 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2161 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2221 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2281 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2341 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2401 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2461 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2521 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     2581 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     2641 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     2701 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2761 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     2821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2941 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3001 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3061 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3121 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3181 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3241 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3301 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3361 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3421 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3481 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3541 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3601 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     3661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3721 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3781 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3841 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3901 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3961 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4021 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4081 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4141 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4201 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4261 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4321 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4381 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4441 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4501 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4561 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4621 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4681 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4741 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4801 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4861 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4921 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4981 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5041 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5101 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5161 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5221 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5281 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5341 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5401 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5461 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5521 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5581 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5641 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5701 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5761 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     5821 acaaaagctg gagctgcaag cggtaccgtc gacctgcagg ttgacattga ttattgacta
     5881 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5941 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     6001 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     6061 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6121 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6181 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     6241 tggcgcgcca tgcagggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     6301 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6421 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6481 ccatagtccc ttaagaggcg cgccttaatg tagtcttatg caatactctt gtagtcttgc
     6541 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6601 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6661 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     6721 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6781 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6841 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6901 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6961 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7021 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7081 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7141 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7201 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7261 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7321 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7381 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     7441 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7501 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7561 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7621 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7681 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7741 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     7801 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     7861 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     7921 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     7981 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8041 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8101 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8161 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacaattc
     8221 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     8281 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8341 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     8401 ccactttggc gccggctcga gtttactccc tatcagtgat agagaacgta taaggagttt
     8461 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
     8521 tatctacagt ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag
     8581 tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt
     8641 actccctatc agtgatagag aacgtataag ctttaggcgt gtacggtggg cgcctataaa
     8701 agcagagctc gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct
     8761 tataccaact ttccgtacca cttcctaccc tcgtaaagga tccgccacca tggtgagcaa
     8821 gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     8881 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     8941 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     9001 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     9061 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     9121 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     9181 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     9241 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     9301 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     9361 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     9421 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     9481 gttcctgtac ggcatggacg agctgtacaa gtgagctagc cgtctcagat cctgtttgaa
     9541 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
     9601 acttcttcag gttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg
     9661 atctcgcttg ggcgagagta agtagtgaag ccacagatgt acttactctc gcccaagcga
     9721 gagtgcctac tgcctcggaa ttcaaggggc tactttagga gcaattatct tgtttactaa
     9781 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
     9841 attaaatcac tttaccggta acgcgtaatt ctaccgggta ggggaggcgc ttttcccaag
     9901 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     9961 ctggcctcgc acacattcca catccaccgg aaggcgccaa ccggctccgt tctttggtgg
    10021 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
    10081 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtgtc actagtctcg tgcagatgga
    10141 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
    10201 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggc
//
