LOCUS       sequence-338347-        8907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human EEA1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Wu H, Voeltz GK
  TITLE     Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact 
            Sites.
  JOURNAL   Dev Cell. 2021 Jan 11;56(1):52-66.e7. doi: 
            10.1016/j.devcel.2020.12.014.
  PUBMED    33434526
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2020.12"; journalName: "Dev Cell"; date: 
            "2021-01-11- 11"; volume: "56"; issue: "1"; pages: "52-66.e7"
COMMENT     Sequence Label: mScarlet-EEA1
FEATURES             Location/Qualifiers
     source          1..8907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        98..401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        402..605
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     556..576
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             650..1345
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(783..801)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1051..1070
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1367..5602
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    5732..5853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5769..5788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5823..5842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5860..6315)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5997..6018)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6209..6228
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6342..6446
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6448..6805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6448..6639
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6471..6491)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6656..6791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6840..7634
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6840..7634
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6894..6913)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7504..7523
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7822..7841)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7866..7913
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8242..8830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8731..8750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
       61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      421 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      481 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      541 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      601 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
      661 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
      721 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      781 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
      841 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
      901 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      961 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
     1021 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
     1081 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
     1141 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
     1201 caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc
     1261 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
     1321 cggcggcatg gacgagctgt acaagtccgg actcagatct cgagctatgt taaggaggat
     1381 tttacagagg actcctggga gagttggctc tcaaggttct gatttagatt catcagcaac
     1441 tcctataaac acagtggacg tcaataatga aagctcttca gagggtttca tatgtcccca
     1501 gtgtatgaaa tctcttggat ctgctgatga acttttcaaa cattatgaag ctgttcatga
     1561 tgctggtaat gactcaggtc atggaggaga gtctaatctt gctttgaagc gagatgatgt
     1621 aacactgctc agacaagagg tccaagacct acaggcttca cttaaggaag aaaaatggta
     1681 ctcggaagaa ttaaagaagg aattagaaaa atatcaaggg ctgcagcagc aagaggccaa
     1741 acctgatggg ttggtgactg attcatcagc agaactacag tctttggaac agcaattaga
     1801 agaagcccaa acagaaaatt ttaatattaa gcaaatgaaa gacttatttg aacagaaagc
     1861 agcccaactt gctactgaaa ttgcagatat aaagtcaaag tatgatgaag aaaggagtct
     1921 tcgagaagct gctgaacaaa aagtgacacg tctgacagaa gaattaaaca aagaggcaac
     1981 tgtaattcaa gatctgaaga cggaactgct tcagagacct ggtatagaag atgttgccgt
     2041 gctaaagaaa gaactggtcc aagttcaaac actaatggat aacatgacct tggaacgtga
     2101 gcgagaatct gaaaaactca aagatgaatg caaaaaattg cagtcacaat atgctagctc
     2161 agaggccaca ataagccagc taaggagtga acttgccaaa ggcccccagg aagttgctgt
     2221 atatgtacag gaactacaaa aactgaaaag ttcagttaat gaattaacac aaaaaaatca
     2281 gaccttgaca gaaaacttgc tgaaaaaaga acaagactat actaagttag aggagaaaca
     2341 taatgaagaa tctgtgagta aaaagaatat tcaggcaacc cttcatcaaa aagacctaga
     2401 ttgtcaacag cttcagtcaa gattgtctgc atctgaaacc tcactgcata gaatacatgt
     2461 agaactaagt gaaaaaggag aagctactca aaagctcaaa gaagaattat ctgaggtaga
     2521 gaccaagtac cagcatctaa aggcggagtt taagcagcta caacaacaga gagaagaaaa
     2581 ggagcagcat gggttacaac ttcaaagtga aattaatcaa ttacatagca aacttctgga
     2641 gacagagcgc caactagggg aagctcatgg taggctgaag gaacagagac agctttcaag
     2701 tgaaaagttg atggataaag aacaacaagt ggctgattta caactcaaac tttctcggtt
     2761 agaagagcag ttgaaggaaa aagttacaaa ttctacagaa ttgcagcatc aattagataa
     2821 aacaaagcaa cagcatcaag aacaacaggc tcttcagcaa agcaccacgg caaaacttcg
     2881 agaagctcag aatgatttgg aacaagttct acgtcaaatt ggcgataagg accaaaagat
     2941 ccagaacctt gaagctttat tacagaagag taaagaaaat atttcattac tagaaaaaga
     3001 aagagaagat ctttatgcaa aaattcaggc tggtgaagga gagactgctg ttcttaacca
     3061 gttacaagaa aaaaaccata cactacagga gcaagtaact caactaacag agaagctgaa
     3121 gaatcagtca gaaagtcata aacaagccca ggagaatttg catgaccagg tacaagagca
     3181 gaaggcacat cttagagctg cacaagaccg tgtcctttcc ctagaaacta gtgtcaatga
     3241 attaaatagt caattaaatg aaagcaagga gaaggtctcc cagcttgaca tacagattaa
     3301 agccaaaacc gaactattac tatcagcaga agcagcaaaa actgctcaaa gagctgatct
     3361 tcagaatcat ttggacacag ctcaaaatgc attacaagat aaacagcagg agttaaataa
     3421 gattactact cagttggatc aggtcactgc aaagttacaa gacaagcaag aacattgcag
     3481 tcagctggaa agtcatctta aagaatataa agagaaatac ctctctttag aacagaaaac
     3541 cgaagagcta gaaggtcaaa ttaagaaact agaagctgat agtcttgaag ttaaagcaag
     3601 caaggagcag gctttgcaag atctacaaca gcaaagacag ctgaacacag atttagagct
     3661 cagagccaca gaattgagta aacaacttga aatggagaag gaaatagtat ccagtacaag
     3721 attggatcta cagaaaaaat ctgaagccct tgaaagtatc aagcaaaagc ttaccaagca
     3781 agaggaagaa aaacaaatcc tgaaacaaga ttttgaaact ttaagtcaag aaacaaagat
     3841 tcagcatgag gaattgaata acagaattca aacaacagta acagaactac aaaaagtgaa
     3901 aatggagaaa gaagctttaa tgacagagct ttctacagta aaggacaaac tatcaaaagt
     3961 ttctgattct ttgaaaaact ctaaaagtga atttgaaaag gagaatcaga aaggaaaagc
     4021 cgctatatta gacttggaaa aaacttgcaa agaattaaag catcaacttc aagtgcagat
     4081 ggaaaacaca cttaaggaac agaaggaact gaaaaagtca cttgaaaaag agaaggaggc
     4141 ttctcatcag ttgaaattgg aactcaattc aatgcaggaa caacttatac aggcccagaa
     4201 tactttaaaa caaaatgaaa aagaagagca acaacttcag gggaacataa atgagctaaa
     4261 gcaatcaagt gaacagaaga aaaaacaaat tgaagcactc caaggagagc ttaaaattgc
     4321 tgttttacag aagacagagc ttgagaataa actacagcag cagttaacac aggcagccca
     4381 ggaacttgca gcagagaaag agaaaatatc agtattacaa aacaactatg aaaaaagtca
     4441 ggaaactttc aaacagcttc aatctgattt ctatgggagg gaatctgaac ttctagccac
     4501 caggcaagat cttaagtctg tagaagagaa gctttctcta gcacaggagg acttgatttc
     4561 aaacagaaat caaattggaa atcaaaataa attgattcaa gaactgaaga ctgccaaggc
     4621 tacattggag caggattcag caaagaaaga acagcaattg caggagcgat gtaaagcact
     4681 acaagacatt cagaaagaaa agtcactgaa agaaaaagaa ctggtaaatg agaagtctaa
     4741 attggcagag atagaagaaa ttaaatgtag acaagaaaaa gaaatcacta aactaaacga
     4801 agaactcaag tcccacaaac tagaaagcat aaaggagata acaaatctta aagatgctaa
     4861 acagcttcta attcagcaga aattagaact tcaaggaaaa gcggactccc tgaaggcagc
     4921 tgttgaacag gagaagagaa atcagcagat actaaaagac caggtgaaaa aggaagaaga
     4981 ggagctgaag aaagaattta ttgagaaaga agctaagttg cattccgaaa taaaagaaaa
     5041 ggaagtagga atgaagaagc atgaagaaaa tgaggctaaa cttaccatgc agattacagc
     5101 attaaatgaa aacttaggca ctgtgaagaa ggagtggcaa tctagtcaac ggagagttag
     5161 tgagcttgag aaacaaacgg atgacttacg gggtgaaatt gcagtattag aagcaacggt
     5221 tcagaataat caagatgaaa ggagagcact actggaaaga tgtcttaaag gagaaggtga
     5281 aatagaaaag cttcaaacca aagtattaga attgcaaaga aagctggata atacaactgc
     5341 agcagtgcag gagctgggca gagaaaacca atcacttcag atcaaacata cacaagcgtt
     5401 gaatagaaag tgggccgaag acaatgaagt acaaaactgt atggcctgtg ggaaaggctt
     5461 ttcagtaaca gtgagacggc atcactgccg acagtgtgga aatatcttct gtgctgaatg
     5521 ttcagccaaa aatgccttaa ctccttcctc caagaagcct gttcgtgtct gtgatgcatg
     5581 tttcaatgac ttgcaaggat aaggatccac cggatctaga taactgatca taatcagcca
     5641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5821 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     5881 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5941 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6001 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6061 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6121 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6181 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6241 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6301 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6361 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6421 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6481 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6541 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6601 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6781 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     6841 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6901 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6961 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7021 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7081 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7141 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7201 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7261 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7321 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7381 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7441 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7501 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7561 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7621 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7681 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7741 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7801 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     7861 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     7921 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     7981 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8041 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8101 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8161 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8221 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8281 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8341 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8401 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8461 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8521 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8581 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8641 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8701 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8761 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8821 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8881 tgctcacatg ttctttcctg cgttatc
//
