LOCUS       sequence-338373-       12749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of BCL6-GFP R28A mutant fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Slabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, 
            Donovan KA, Sperling AS, Hunkeler M, Tsai JM, Sharma R, Guirguis A, 
            Zou C, Chudasama P, Gasser JA, Miller PG, Scholl C, Frohling S, 
            Nowak RP, Fischer ES, Ebert BL
  TITLE     Small-molecule-induced polymerization triggers degradation of BCL6.
  JOURNAL   Nature. 2020 Dec;588(7836):164-168. doi: 10.1038/s41586-020-2925-1. 
            Epub 2020 Nov 18.
  PUBMED    33208943
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2925-1"; journalName: "Nature"; date: "2020-12";
            volume: "588"; issue: "7836"; pages: "164-168"
COMMENT     Sequence Label: BCL6-GFP R28A in Artichoke
FEATURES             Location/Qualifiers
     source          1..12749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1737..2143
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2202..2226
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4348..4372)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4437..4439,4440..5153)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4437..4439,4440..5150)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4479..4500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4740..4759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5087..5108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5177..5751,5752..5754,5755..5763)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5344..5361)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5571..5590
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5773..5782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5779..6489
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5924..5942)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6192..6211
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6545..6662
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6796..7974
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7027..7965
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7922..7942
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7987..8586
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7987..8006)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8483..8503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8602..9190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8655..8675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9073..9084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9262..9495
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9573..9694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9610..9629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9664..9683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9734..9869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9889..9908)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9890..9908)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9918..9935)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9918..9934)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9927..9949)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10076..10531
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10163..10182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10373..10394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10557..10661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10662..10730,10731..11522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10880..10899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11693..12281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12182..12201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12435..12452
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12569..12590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12605..12610,12611..12628,12629..12635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12643..12659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12648..12670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12667..12683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12667..12683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12702..12722
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12704..12722
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct attccagcac atatgagcta gctgcagtaa
     1741 cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg
     1801 cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg
     1861 ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag
     1921 aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
     1981 tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc
     2041 tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
     2101 cctcactcgg cgcgccagtc ctccgacaga ctgagtcggc cggtcgaatc aagcttatcg
     2161 ataccgtcga ctccggaata gccaccatgg agacggatca aacaagtttg tacaaaaaag
     2221 ttggcaccat ggcctcgccg gctgacagct gtatccagtt cacccgccat gccagtgatg
     2281 ttcttctcaa ccttaatcgt ctccggagtg cagacatctt gactgatgtt gtcattgttg
     2341 tgagccgtga gcagtttaga gcccataaaa cggtcctcat ggcctgcagt ggcctgttct
     2401 atagcatctt tacagaccag ttgaaatgca accttagtgt gatcaatcta gatcctgaga
     2461 tcaaccctga gggattctgc atcctcctgg acttcatgta cacatctcgg ctcaatttgc
     2521 gggagggcaa catcatggct gtgatggcca cggctatgta cctgcagatg gagcatgttg
     2581 tggacacttg ccggaagttt attaaggcca gtgaagcaga gatggtttct gccatcaagc
     2641 ctcctcgtga agagttcctc aacagccgga tgctgatgcc ccaagacatc atggcctatc
     2701 ggggttgtga ggtggtggag aacaacctgc cactgaggag cgcccctggg tgtgagagca
     2761 gagcctttgc ccccagcctg tacagtggcc tgtccacacc gccagcctct tattccatgt
     2821 acagccacct ccctgtcagc agcctcctct tctccgatga ggagtttcgg gatgtccgga
     2881 tgcctgtggc caaccccttc cccaaggagc gggcactccc atgtgatagt gccaggccag
     2941 tccctggtga gtacagccgg ccgactttgg aggtgtcccc caatgtgtgc cacagcaata
     3001 tctattcacc caaggaaaca atcccagaag aggcacgaag tgatatgcac tacagtgtgg
     3061 ctgagggcct caaacctgct gccccctcag cccgaaatgc cccctacttc ccttgtgaca
     3121 aggccagcaa agaagaagag agaccctcct cggaagatga gattgccctg catttcgagc
     3181 cccccaatgc acccctgaac cggaagggtc tggttagtcc acagagcccc cagaaatctg
     3241 actgccagcc caactcgccc acagagtcct gcagcagtaa gaatgcctgc atcctccagg
     3301 cttctggctc ccctccagcc aagagcccca ctgaccccaa agcctgcaac tggaagaaat
     3361 acaagttcat cgtgctcaac agcctcaatc agaatgccaa accagagggg cctgagcagg
     3421 ctgagctggg ccgcctttcc ccacgagcct acacggcccc acctgcctgc cagccaccca
     3481 tggagcctga gaaccttgac ctccagtccc caaccaagct gagtgccagc ggggaggact
     3541 ccaccatccc acaagccagc cggctcaata acatcgttaa caggtccatg acgggctctc
     3601 cccgcagcag cagcgagagc cactcaccac tctacatgca ccccccgaag tgcacgtcct
     3661 gcggctctca gtccccacag catgcagaga tgtgcctcca caccgctggc cccacgttcc
     3721 ctgaggagat gggagagacc cagtctgagt actcagattc tagctgtgag aacggggcct
     3781 tcttctgcaa tgagtgtgac tgccgcttct ctgaggaggc ctcactcaag aggcacacgc
     3841 tgcagaccca cagtgacaaa ccctacaagt gtgaccgctg ccaggcctcc ttccgctaca
     3901 agggcaacct cgccagccac aagaccgtcc ataccggtga gaaaccctat cgttgcaaca
     3961 tctgtggggc ccagttcaac cggccagcca acctgaaaac ccacactcga attcactctg
     4021 gagagaagcc ctacaaatgc gaaacctgcg gagccagatt tgtacaggtg gcccacctcc
     4081 gtgcccatgt gcttatccac actggtgaga agccctatcc ctgtgaaatc tgtggcaccc
     4141 gtttccggca ccttcagact ctgaagagcc acctgcgaat ccacacagga gagaaacctt
     4201 accattgtga gaagtgtaac ctgcatttcc gtcacaaaag ccagctgcga cttcacttgc
     4261 gccagaagca tggcgccatc accaacacca aggtgcaata ccgcgtgtca gccactgacc
     4321 tgcctccgga gctccccaaa gcctgcttgc caactttctt gtacaaagtg gttcgatacg
     4381 tctcagctga agctgctgca aaggaagctg cagctaagga ggctgcagct aaggctgtga
     4441 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4501 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4561 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4621 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4681 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4741 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4801 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4861 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4921 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5041 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5101 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaatgcatg
     5161 agtaactgag gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5221 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5281 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5341 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5401 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5461 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5521 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5581 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5641 atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5701 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5761 accctggaat tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     5821 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     5881 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     5941 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     6001 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     6061 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     6121 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     6181 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     6241 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     6301 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     6361 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     6421 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     6481 tacaagtaaa ctagtaagct tggcgtaact agatcttgag acaaatggca gtattcatcc
     6541 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     6601 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     6661 ttcgggttta ttacagggac agcagagatc cactttgggc tcgagggggc ccgggtgcaa
     6721 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     6781 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6841 cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg
     6901 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6961 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     7021 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     7081 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     7141 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     7201 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     7261 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     7321 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     7381 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     7441 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     7501 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     7561 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     7621 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     7681 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     7741 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     7801 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     7861 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     7921 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg
     7981 gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     8041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     8101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     8161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     8221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     8341 aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt
     8401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8581 gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8641 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8701 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8761 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8821 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8881 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8941 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9001 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9061 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9121 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9181 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9241 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9301 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9361 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9421 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9481 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9541 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9601 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9661 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9721 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9781 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9841 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9901 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9961 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10021 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10501 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10561 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10621 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10681 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10741 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10801 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10861 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10921 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10981 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    11041 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    11101 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    11161 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    11221 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11281 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11341 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11401 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11461 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11521 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    12001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    12121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12361 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12481 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12601 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12661 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12721 gggaacaaaa gctggagctg caagcttaa
//
