LOCUS       sequence-338377-       11272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted
            into a FRT site by co-transfection with a plasmid encoding Flp 
            recombinaase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Alekseyenko AA, Walsh EM, Zee BM, Pakozdi T, Hsi P, Lemieux ME, Dal 
            Cin P, Ince TA, Kharchenko PV, Kuroda MI, French CA
  TITLE     Ectopic protein interactions within BRD4-chromatin complexes drive 
            oncogenic megadomain formation in NUT midline carcinoma.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4184-E4192. doi: 
            10.1073/pnas.1702086114. Epub 2017 May 8.
  PUBMED    28484033
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1702086114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-05-23- 23"; volume: "114"; issue: "21"; pages: 
            "E4184-E4192"
COMMENT     Sequence Label: pcDNA5 frt/to N-BioTAP-C-BRD4-NUT
FEATURES             Location/Qualifiers
     source          1..11272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             849..1022
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     protein_bind    1317..1341
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6916..6940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6990..7007)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6996..7220
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7353..7372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7504..7551
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7559..8581
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7559..8581
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8711..8832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8748..8767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8802..8821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8881..8897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8881..8897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8894..8916)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8905..8921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8929..8935,8936..8953,8954..8959))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8974..8995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9112..9129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9283..9871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9363..9382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10042..10833,10834..10902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10665..10684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10903..11007)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11082..11101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc cagcacagtg gcggccgcat ggcaggcctt gcgcaacacg
      841 atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac
      901 atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc
      961 caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caggcgccga
     1021 aagtagacgc gaattgtgat atacctacaa ctgcttctct cgagagcggc tcctcggctg
     1081 gaaaggccgg tgaaggtgaa atccctgccc ctcttgctgg taccgtttct aagatactgg
     1141 taaaagaagg tgacactgtt aaagctggtc aaacagttct ggtgctggag gctatgaaaa
     1201 tggagacaga aattaacgct cctactgacg gaaaagttga aaaggtgtta gttaaggaaa
     1261 gagatgctgt tcaaggtggt caaggtctaa tcaagatcgg cgtttcgagc ccatcaacaa
     1321 gtttgtacaa aaaagcaggc tccgcggccg cccccttcac catgtctgcg gagagcggcc
     1381 ctgggacgag attgagaaat ctgccagtaa tgggggatgg actagaaact tcccaaatgt
     1441 ctacaacaca ggcccaggcc caaccccagc cagccaacgc agccagcacc aaccccccgc
     1501 ccccagagac ctccaaccct aacaagccca agaggcagac caaccaactg caatacctgc
     1561 tcagagtggt gctcaagaca ctatggaaac accagtttgc atggcctttc cagcagcctg
     1621 tggatgccgt caagctgaac ctccctgatt actataagat cattaaaacg cctatggata
     1681 tgggaacaat aaagaagcgc ttggaaaaca actattactg gaatgctcag gaatgtatcc
     1741 aggacttcaa cactatgttt acaaattgtt acatctacaa caagcctgga gatgacatag
     1801 tcttaatggc agaagctctg gaaaagctct tcttgcaaaa aataaatgag ctacccacag
     1861 aagaaaccga gatcatgata gtccaggcaa aaggaagagg acgtgggagg aaagaaacag
     1921 ggacagcaaa acctggcgtt tccacggtac caaacacaac tcaagcatcg actcctccgc
     1981 agacccagac ccctcagccg aatcctcctc ctgtgcaggc cacgcctcac cccttccctg
     2041 ccgtcacccc ggacctcatc gtccagaccc ctgtcatgac agtggtgcct ccccagccac
     2101 tgcagacgcc cccgccagtg cccccccagc cacaaccccc acccgctcca gctccccagc
     2161 ccgtacagag ccacccaccc atcatcgcgg ccaccccaca gcctgtgaag acaaagaagg
     2221 gagtgaagag gaaagcagac accaccaccc ccaccaccat tgaccccatt cacgagccac
     2281 cctcgctgcc cccggagccc aagaccacca agctgggcca gcggcgggag agcagccggc
     2341 ctgtgaaacc tccaaagaag gacgtgcccg actctcagca gcacccagca ccagagaaga
     2401 gcagcaaggt ctcggagcag ctcaagtgct gcagcggcat cctcaaggag atgtttgcca
     2461 agaagcacgc cgcctacgcc tggcccttct acaagcctgt ggacgtggag gcactgggcc
     2521 tacacgacta ctgtgacatc atcaagcacc ccatggacat gagcacaatc aagtctaaac
     2581 tggaggcccg tgagtaccgt gatgctcagg agtttggtgc tgacgtccga ttgatgttct
     2641 ccaactgcta taagtacaac cctcctgacc atgaggtggt ggccatggcc cgcaagctcc
     2701 aggatgtgtt cgaaatgcgc tttgccaaga tgccggacga gcctgaggag ccagtggtgg
     2761 ccgtgtcctc cccggcagtg ccccctccca ccaaggttgt ggccccgccc tcatccagcg
     2821 acagcagcag cgatagctcc tcggacagtg acagttcgac tgatgactct gaggaggagc
     2881 gagcccagcg gctggctgag ctccaggagc agctcaaagc cgtgcacgag cagcttgcag
     2941 ccctctctca gccccagcag aacaaaccaa agaaaaagga gaaagacaag aaggaaaaga
     3001 aaaaagaaaa gcacaaaagg aaagaggaag tggaagagaa taaaaaaagc aaagccaagg
     3061 aacctcctcc taaaaagacg aagaaaaata atagcagcaa cagcaatgtg agcaagaagg
     3121 agccagcgcc catgaagagc aagccccctc ccacgtatga gtcggaggaa gaggacaagt
     3181 gcaagcctat gtcctatgag gagaagcggc agctcagctt ggacatcaac aagctccccg
     3241 gcgagaagct gggccgcgtg gtgcatatca tccagtcacg ggagccctcc ctgaagaatt
     3301 ccaaccccga cgagattgaa atcgactttg agaccctgaa gccgtccaca ctgcgtgagc
     3361 tggagcgcta tgtcacctcc tgtttgcgga agaaaaggaa acctcaagct gagaaagttg
     3421 atgtgattgc cggctcctcc aagatgaagg gcttctcgtc ctcagagtcg gagagctcca
     3481 gtgagtccag ctcctctgac agcgaagact ccgaaacagc atctgcattg ccgggaccgg
     3541 atatgagcat gaaacctagt gccgccctgt ctccatcccc tgcacttccc tttctcccac
     3601 caacttctga cccaccagac cacccaccca gggagccacc tccacagccc atcatgcctt
     3661 cagtattctc tccagacaac cctctgatgc tctctgcttt ccccagctca ctgttggtga
     3721 caggggacgg gggcccttgc ctcagtgggg ctggggctgg caaggtcatt gtcaaagtca
     3781 agacagaagg ggggtcagct gagccctctc aaactcagaa ctttatcctt actcagactg
     3841 ccctcaattc gactgccccg ggcactccct gtggaggcct tgagggtcct gcacctccat
     3901 ttgtgacagc atctaatgtg aagaccattc tgccctctaa ggctgttggt gtcagccagg
     3961 agggtcctcc aggccttccg cctcagcctc caccaccagt tgctcaactg gtccccattg
     4021 tgcccctgga aaaagcttgg ccagggccac atgggacaac cggggaagga ggtcctgtgg
     4081 ccactctatc caagccttcc ctaggtgacc gctccaaaat ttccaaggac gtttatgaga
     4141 acttccgtca gtggcagcgt tacaaagcct tggcccggag gcacctatcc cagagtcctg
     4201 acacagaagc tctttcctgt tttcttatcc cagtgcttcg ttccctggcc cggctgaagc
     4261 ccactatgac cctggaggag ggactgccat tggctgtgca ggagtgggag cacaccagca
     4321 actttgaccg gatgatcttt tatgagatgg cagaaaggtt catggagttt gaggctgagg
     4381 agatgcagat tcagaacaca cagctgatga atgggtctca gggcctgtct cctgcaaccc
     4441 ctttgaaact tgatcctcta gggcccctgg cctctgaggt ttgccagcag ccagtgtaca
     4501 ttccgaagaa ggcagcttct aaaacaagag ctcctcgccg gcgtcagcgt aaagcccaga
     4561 gacctcctgc tcctgaggca cccaaggaga tcccaccaga agctgtgaag gagtatgttg
     4621 acatcatgga atggctggtg gggactcact tggccactgg ggagtcagat ggaaaacaag
     4681 aggaagaagg gcagcagcag gaggaggaag ggatgtatcc agatccaggt ctcctgagct
     4741 acatcaatga gctgtgttct cagaaggtct ttgtctccaa ggtggaggct gtcattcacc
     4801 ctcaatttct ggcagatctg ctgtccccag aaaaacagag agatcccttg gccttaattg
     4861 aggagctaga gcaagaagaa ggactcactc ttgcccagct ggtccagaag cgactcatgg
     4921 ccttggaaga ggaggaagat gcagaggcgc ctccaagttt cagtggcgct cagttggact
     4981 caagtccttc tggttctgtt gaggatgaag atggggatgg gcggcttcgg ccctcacctg
     5041 ggcttcaggg ggctgggggc gccgcttgcc ttggaaaggt ttcttcttca ggaaaacggg
     5101 caagagaagt gcatggtggg caggagcaag ccctagatag ccccagaggg atgcacaggg
     5161 atgggaacac tctgccatcc cccagcagct gggacctgca gccagaactt gcagctccac
     5221 agggaactcc gggacccttg ggtgtggaga ggagagggtc tgggaaggtt ataaaccagg
     5281 tatctctaca tcaggatggc catctaggag gcgctgggcc tcctgggcac tgcctggtgg
     5341 ctgataggac ttcagaggct ctgccccttt gttggcaggg aggcttccag cctgagagca
     5401 ctcccagttt ggatgctgga cttgcagagc tggctcctct gcaaggacaa gggttagaaa
     5461 agcaagtcct gggattgcag aaaggacaac aaacaggggg tcgtggagtg cttcctcaag
     5521 ggaaggagcc tttagcagtg ccctgggaag gctcttcagg agccatgtgg ggagatgaca
     5581 gaggtacccc catggctcag agttatgatc agaatccttc ccctagagca gctggggaga
     5641 gggacgatgt ctgtctcagc ccaggagttt ggctgagcag tgagatggat gctgtaggct
     5701 tggagctgcc tgtacaaata gaggaggtca tagagagctt ccaagttgag aagtgtgtaa
     5761 ctgagtatca ggaaggctgc cagggactgg gctccagggg caacatttcc ctgggtcctg
     5821 gagaaaccct agtacctggg gatacggaga gcagtgtgat tccctgtgga ggcacagttg
     5881 cggcagctgc cctagaaaag agaaactatt gcagcttgcc aggacctttg agggccaaca
     5941 gcccaccctt gaggtccaaa gaaaatcaag aacagagctg tgaaaccgta gggcatccca
     6001 gtgatctgtg ggcagaaggc tgcttcccat tgctagaaag tggtgattcc acactggggt
     6061 cttccaaaga aacccttcca cccacatgcc aaggcaatct ccttatcatg gggactgagg
     6121 atgcctcctc cttgcctgaa gccagtcaag aggcagggag cagaggcaat tccttttctc
     6181 ctctgttgga aaccatagaa cctgtcaaca tactagatgt taaagatgac tgtggcctcc
     6241 aactaagggt cagcgaggac acctgcccac tgaatgttca ttcttatgac ccccaaggag
     6301 aaggcagggt ggatcctgat ctgtccaagc ctaaaaacct tgctccttta caagagagtc
     6361 aggagtctta cacaactggg actcccaaag caacatcttc tcaccagggc cttggaagca
     6421 ctttgcctag aaggggaacc aggaatgcca tagttccgag agaaacttct gttagtaaaa
     6481 cacacaggtc agcagacagg gccaaaggaa aggagaaaaa gaaaaaggaa gcagaggaag
     6541 aggatgagga actctccaac tttgcttacc tcttggcctc taaacttagc ctctcaccaa
     6601 gggagcatcc cctcagtcct caccatgcct caggaggtca gggcagccag agagcatccc
     6661 acctgctccc tgctggagca aaaggcccca gcaaacttcc atatcctgtt gccaagtctg
     6721 ggaagcgagc tctagctgga ggtccagccc ctactgaaaa gacaccccac tcaggagctc
     6781 aacttggggt ccccagggag aaacccctag ctctgggagt agttcgaccc tcacagcctc
     6841 gtaaaaggcg gtgtgacagt tttgtcacgg gcagaaggaa gaaacgacgt cgtagccaga
     6901 agggtgggcg cgccgaccca gctttcttgt acaaagtggt ttgcgcatag ctaggtagcc
     6961 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7021 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7081 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7141 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7201 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7261 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7321 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     7501 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     7561 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     7621 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     7681 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     7741 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     7801 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     7861 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     7921 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     7981 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     8041 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     8101 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     8161 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     8221 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     8281 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     8341 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     8401 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     8461 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     8521 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     8581 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8641 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8701 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8821 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8881 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8941 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9001 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9061 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11041 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    11101 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    11161 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    11221 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
