LOCUS       sequence-338393-        2232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Drp1 Isoform 3 with K557A, K560A mutations in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
  TITLE     Dynamin-related protein 1 has membrane constricting and severing 
            abilities sufficient for mitochondrial and peroxisomal fission.
  JOURNAL   Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
  PUBMED    30531964
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-7"; volume: "9(1):5239. doi"; pages: " 
            10.1038/s41467-018-07543-w"
COMMENT     Sequence Label: 6xHis(TEV)-Drp1(K557A,K560A)-StrepII
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2186..2209
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacgaaaat ctgtatttcc aaggctcgat ggaggcgcta attcctgtca taaacaagct
      121 ccaggacgtc ttcaacacgg tgggcgccga catcatccag ctgcctcaaa tcgtcgtagt
      181 gggaacgcag agcagcggaa agagctcagt gctagaaagc ctggtgggga gggacctgct
      241 tcccagaggt actggaattg tcacccggag acctctcatt ctgcaactgg tccatgtttc
      301 acaagaagat aaacggaaaa caacaggaga agaaaatggg gtggaagcag aagaatgggg
      361 taaatttctt cacaccaaaa ataagcttta cacggatttt gatgaaattc gacaagaaat
      421 tgaaaatgaa acagaaagaa tttcaggaaa taataaggga gtaagccctg aaccaattca
      481 tcttaagatt ttttcaccca acgttgtcaa tttgacactt gtggatttgc caggaatgac
      541 caaggtgcct gtaggtgatc aacctaagga tattgagctt caaatcagag agctcattct
      601 tcggttcatc agtaatccta attccattat cctcgctgtc actgctgcta atacagatat
      661 ggcaacatca gaggcactta aaatttcaag agaggtagat ccagatggtc gcagaaccct
      721 agctgtaatc actaaacttg atctcatgga tgcgggtact gatgccatgg atgtattgat
      781 gggaagggtt attccagtca aacttggaat aattggagta gttaacagga gccagctaga
      841 tattaacaac aagaagagtg taactgattc aatccgtgat gagtatgctt ttcttcaaaa
      901 gaaatatcca tctctggcca atagaaatgg aacaaagtat cttgctagga ctctaaacag
      961 gttactgatg catcacatca gagattgttt accagagttg aaaacaagaa taaatgttct
     1021 agctgctcag tatcagtctc ttctaaatag ctacggtgaa cccgtggatg ataaaagtgc
     1081 tactttactc caacttatta ccaaatttgc cacagaatat tgtaacacta ttgaaggaac
     1141 tgcaaaatat attgaaactt cggagctatg cggtggtgct agaatttgtt atattttcca
     1201 tgagactttt gggcgaacct tagaatctgt tgatccactt ggtggcctta acactattga
     1261 cattttgact gccattagaa atgctactgg tcctcgtcct gctttatttg tgcctgaggt
     1321 ttcatttgag ttactggtga agcggcaaat caaacgtcta gaagagccca gcctccgctg
     1381 tgtggaactg gttcatgagg aaatgcaaag gatcattcag cactgtagca attacagtac
     1441 acaggaattg ttacgatttc ctaaacttca tgatgccata gttgaagtgg tgacttgtct
     1501 tcttcgtaaa aggttgcctg ttacaaatga aatggtccat aacttagtgg caattgaact
     1561 ggcttatatc aacacaaaac atccagactt tgctgatgct tgtgggctaa tgaacaataa
     1621 tatagaggaa caaaggagaa acaggctagc cagagaatta ccttcagctg tatcacgaga
     1681 caaggttgca tctggaggtg gtggggttgg agatggtgtt caagaaccaa ccacaggcaa
     1741 ctggagagga atgctggcaa cttcagcagc tgaagagtta ttagcagaag aaaaatcaaa
     1801 acccattcca attatgccag ccagtccaca aaaaggtcat gccgtgaacc tgctagatgt
     1861 gccagttcct gttgcacgaa aactatctgc tcgggaacag cgagattgtg aggttattga
     1921 acgactcatt aaatcatatt ttctcattgt cagaaagaat attcaagaca gtgtgccaaa
     1981 ggcagtaatg cattttttgg ttaatcatgt gaaagacact cttcagagtg agctagtagg
     2041 ccagctgtat aaatcatcct tattggatga tcttctgaca gaatctgagg acatggcaca
     2101 gcgcaggaaa gaagcagctg atatgctaaa ggcattacaa ggagccagtc aaattattgc
     2161 tgaaatccgg gagactcatc tttggtggtc tcatcctcag ttcgaaaaat aagatccggc
     2221 tgctaacaaa gc
//
