LOCUS       sequence-338395-        2452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pombe Drp1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
  TITLE     Dynamin-related protein 1 has membrane constricting and severing 
            abilities sufficient for mitochondrial and peroxisomal fission.
  JOURNAL   Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
  PUBMED    30531964
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-7"; volume: "9(1):5239. doi"; pages: " 
            10.1038/s41467-018-07543-w"
COMMENT     Sequence Label: 6xHis(TEV)-yDrp1-StrepII
FEATURES             Location/Qualifiers
     source          1..2452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             2426..2449
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacgaaaat ctgtatttcc aaatggaaca acttattcca ttggtgaatc aacttcaaga
      121 ccttgtctac aacacaattg ggtcagattt tttggattta ccatccatag tggtcgttgg
      181 gtcccaatct tgtggaaaat cttctgtatt ggaaaatatt gttggtaaag attttttacc
      241 tcgtggtacc ggaattgtca ctagaagacc cttgatacta caactcatca acttgaagcg
      301 caagaccaaa aataaccatg atgaggaaag tacttctgac aacaattcag aagaaacaag
      361 tgctgctggg gaaacaggca gtcttgaagg aattgaagaa gattctgatg aaattgaaga
      421 ttatgctgaa tttttgcata tccccgatac caaatttacc gacatgaaca aagttcgcgc
      481 agagattgag aatgagactc tacgggttgc tggtgcaaac aaaggaatta acaaattgcc
      541 aatcaatcta aaaatttatt ctactcgcgt tttgaactta actttaattg atttgcctgg
      601 gcttacaaaa attcccgttg gagatcagcc tactgatatt gaggctcaaa cacgctctct
      661 tattatggaa tacatcagcc gtcccaattc cattatactt gctgtatctc ctgccaactt
      721 tgacattgtc aacagtgagg gcctaaaact agcgaggtct gttgatccca agggtaagcg
      781 cactattggt gttcttacca agcttgacct tatggatcaa ggtaccaatg caatggacat
      841 attaagcggt cgcgtttatc cccttaagct gggtttcgta gcaactgtta accgttccca
      901 gtctgatatt gtatctcaca aatccatgag agatgctttg caatctgagc gctctttttt
      961 tgaacatcat ccggcatatc gtaccattaa agatcgttgt ggaacaccct atcttgccaa
     1021 gacattgagt aatctattgg tatcacatat ccgtgagcgc cttcccgata tcaaagcccg
     1081 ccttagcact ctaatatctc aaactcaaca acaactaaac aattacggtg actttaaact
     1141 ttctgatcag agtcaacgtg gaattatttt attgcaggcg atgaatcgct ttgccaacac
     1201 gttcattgct agtatagatg gaaattcttc aaatattccc acaaaagaac tttcaggtgg
     1261 tgctcgtctt tactccattt tcaataatgt atttactact gcattgaatt caatcgatcc
     1321 tttacaaaac ttatcgacag tcgacatacg tacagctata ttgaatagca caggctctag
     1381 ggctaccttg ttcctttcgg aaatggcttt cgatatattg gtgaagccgc aattaaacct
     1441 tttagcggca ccctgtcatc aatgtgtcga gcttgtttac gaagaattaa tgaaaatatg
     1501 tcattattct ggtgactcag acatatcgca ttttcccaag ttgcaaactg ctctcgtaga
     1561 aaccgttagc gatttactac gagaaaattt aactcctact tattcttttg tagaaagctt
     1621 gattgctatt caaagtgcat atattaatac gaatcatccg gatttcttag gggtgcaggg
     1681 tgcaatggct gtagtattgt caagaaaaga acagaatcgc ttaatgcttt cccaagaaaa
     1741 tgatgaaccc atttcttctg ctttggacac tgtgaagcct gacggaattg agttgtattc
     1801 ttcggatccc gacacttctg ttaagagtat taccaataaa gcgacgaacg agattacaac
     1861 tttgaagagc gatgattcag caaaaatgca gcctttggat gttttggctt ctaaacggta
     1921 taataatgcc tttagcactg aaactgcgga aagaaaaaca tttcttagct atgtctttgg
     1981 tgccaataat gcgactagga aagcaatgag cattgataag tcttctagct atcctttaaa
     2041 tgattctctt tctggaggag atacaaatca caaaaacaac catcctttaa agatgacaga
     2101 tttatcaaat gaagttgaaa caatggcgtt ggaggacatg agtgaaaggg aagaagttga
     2161 agttgatctt attaaagagc taattacttc ttatttcaac ttaactagga agattataat
     2221 tgatcaggta ccaaaggtaa ttatgcattt gctcgtgaac gcctctaaag atgccattca
     2281 aaatcgtttg gttagtaaat tataccgaga ggatttcttt gacaccctcc ttattgaaga
     2341 tgagaacgtc aaaagtgaaa gagaaaaatg tgagcgtctt ttatctgttt acaaccaagc
     2401 aaacaaaatt atttcaacgg ttttttggtc tcatcctcag ttcgaaaaat aa
//
