LOCUS       sequence-338397-        2922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
  TITLE     Dynamin-related protein 1 has membrane constricting and severing 
            abilities sufficient for mitochondrial and peroxisomal fission.
  JOURNAL   Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
  PUBMED    30531964
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-7"; volume: "9(1):5239. doi"; pages: " 
            10.1038/s41467-018-07543-w"
COMMENT     Sequence Label: Drp1-EGFP-StrepII
FEATURES             Location/Qualifiers
     source          1..2922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(2162..2164,2165..2875)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2204..2225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2465..2484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2812..2833
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2876..2899
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagcggct cgatggaggc
       61 gctaattcct gtcataaaca agctccagga cgtcttcaac acggtgggcg ccgacatcat
      121 ccagctgcct caaatcgtcg tagtgggaac gcagagcagc ggaaagagct cagtgctaga
      181 aagcctggtg gggagggacc tgcttcccag aggtactgga attgtcaccc ggagacctct
      241 cattctgcaa ctggtccatg tttcacaaga agataaacgg aaaacaacag gagaagaaaa
      301 tggggtggaa gcagaagaat ggggtaaatt tcttcacacc aaaaataagc tttacacgga
      361 ttttgatgaa attcgacaag aaattgaaaa tgaaacagaa agaatttcag gaaataataa
      421 gggagtaagc cctgaaccaa ttcatcttaa gattttttca cccaacgttg tcaatttgac
      481 acttgtggat ttgccaggaa tgaccaaggt gcctgtaggt gatcaaccta aggatattga
      541 gcttcaaatc agagagctca ttcttcggtt catcagtaat cctaattcca ttatcctcgc
      601 tgtcactgct gctaatacag atatggcaac atcagaggca cttaaaattt caagagaggt
      661 agatccagat ggtcgcagaa ccctagctgt aatcactaaa cttgatctca tggatgcggg
      721 tactgatgcc atggatgtat tgatgggaag ggttattcca gtcaaacttg gaataattgg
      781 agtagttaac aggagccagc tagatattaa caacaagaag agtgtaactg attcaatccg
      841 tgatgagtat gcttttcttc aaaagaaata tccatctctg gccaatagaa atggaacaaa
      901 gtatcttgct aggactctaa acaggttact gatgcatcac atcagagatt gtttaccaga
      961 gttgaaaaca agaataaatg ttctagctgc tcagtatcag tctcttctaa atagctacgg
     1021 tgaacccgtg gatgataaaa gtgctacttt actccaactt attaccaaat ttgccacaga
     1081 atattgtaac actattgaag gaactgcaaa atatattgaa acttcggagc tatgcggtgg
     1141 tgctagaatt tgttatattt tccatgagac ttttgggcga accttagaat ctgttgatcc
     1201 acttggtggc cttaacacta ttgacatttt gactgccatt agaaatgcta ctggtcctcg
     1261 tcctgcttta tttgtgcctg aggtttcatt tgagttactg gtgaagcggc aaatcaaacg
     1321 tctagaagag cccagcctcc gctgtgtgga actggttcat gaggaaatgc aaaggatcat
     1381 tcagcactgt agcaattaca gtacacagga attgttacga tttcctaaac ttcatgatgc
     1441 catagttgaa gtggtgactt gtcttcttcg taaaaggttg cctgttacaa atgaaatggt
     1501 ccataactta gtggcaattg aactggctta tatcaacaca aaacatccag actttgctga
     1561 tgcttgtggg ctaatgaaca ataatataga ggaacaaagg agaaacaggc tagccagaga
     1621 attaccttca gctgtatcac gagacaaggt tgcatctgga ggtggtgggg ttggagatgg
     1681 tgttcaagaa ccaaccacag gcaactggag aggaatgctg aaaacttcaa aagctgaaga
     1741 gttattagca gaagaaaaat caaaacccat tccaattatg ccagccagtc cacaaaaagg
     1801 tcatgccgtg aacctgctag atgtgccagt tcctgttgca cgaaaactat ctgctcggga
     1861 acagcgagat tgtgaggtta ttgaacgact cattaaatca tattttctca ttgtcagaaa
     1921 gaatattcaa gacagtgtgc caaaggcagt aatgcatttt ttggttaatc atgtgaaaga
     1981 cactcttcag agtgagctag taggccagct gtataaatca tccttattgg atgatcttct
     2041 gacagaatct gaggacatgg cacagcgcag gaaagaagca gctgatatgc taaaggcatt
     2101 acaaggagcc agtcaaatta ttgctgaaat ccgggagact catctttggc tcgaggatcc
     2161 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2221 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2281 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2341 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2401 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2461 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2521 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2581 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2641 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2701 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2761 cctgagcacc cagtccaagt tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2821 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtggtc
     2881 tcatcctcag ttcgaaaaat aagatccggc tgctaacaaa gc
//
