LOCUS       sequence-338412-        9798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Guardia CM, Farias GG, Jia R, Pu J, Bonifacino JS
  TITLE     BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional 
            Movement of Lysosomes along Different Microtubule Tracks.
  JOURNAL   Cell Rep. 2016 Nov 15;17(8):1950-1961. doi: 
            10.1016/j.celrep.2016.10.062.
  PUBMED    27851960
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.10"; journalName: "Cell Rep"; date: 
            "2016-11-15- 15"; volume: "17"; issue: "8"; pages: "1950-1961"
COMMENT     Sequence Label: eGFP-KIF1A-Rigor
FEATURES             Location/Qualifiers
     source          1..9798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      288..876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     777..796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1051..1354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1355..1558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1509..1529
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1597..1606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1603..1605,1606..1608,1609..2319)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1603..1605,1606..1608,1609..2319)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1648..1669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1909..1928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2256..2277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3332..3341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6886..6897
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7576..7697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7613..7632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7667..7686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7704..8159)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7841..7862)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8053..8072
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8186..8290
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8292..8649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8292..8483
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8315..8335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8500..8635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8684..9478
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8684..9478
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8738..8757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9348..9367
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9666..9685)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9710..9757
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
       61 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
      121 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      961 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     1021 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1081 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1141 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1201 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1261 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1321 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1381 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1441 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1501 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     1561 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     1621 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1681 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1741 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1801 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1861 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1921 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1981 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2041 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2101 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2161 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2221 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2281 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct
     2341 caagcttcga attctatggc cggggcctct gtgaaggtgg cggtgcgtgt ccgccccttc
     2401 aactcccggg aaatgagccg agactccaag tgcatcattc agatgtctgg aagcaccacc
     2461 actattgtca accccaaaca gcccaaggag acacccaaga gcttcagctt tgattactcc
     2521 tactggtcac atacctcgcc ggaggacatc aactatgcct ctcagaagca ggtctacagg
     2581 gacattgggg aagagatgct acagcatgcc tttgagggct acaatgtgtg catctttgcc
     2641 tatgggcaga cgggggccgg caagtcctac accatgatgg ggaaacagga gaaggaccag
     2701 caaggcatca ttccacagct ctgcgaagac ctcttctctc gaatcaacga caccaccaac
     2761 gacaacatgt cctactctgt ggaggtcagc tacatggaga tttactgtga gcgagtccgt
     2821 gacctcctga accccaagaa caaaggcaac ctgcgtgtga gggagcaccc tctgctgggg
     2881 ccttatgtgg aggacctctc caagctggct gtaacctcct acaatgacat ccaggacctc
     2941 atggactcag ggaacaaggc caggactgtg gcggccacaa acatgaatga gaccagcagc
     3001 cgttctcacg cagtcttcaa catcatcttt acccagaagc gccatgatgc agagaccaac
     3061 atcaccacag agaaagtgag caaaatcagc ctggtggacc tggcagctag tgaacgagcc
     3121 gactccacag gagccaaggg cacgcgactc aaggaggggg ccaacatcaa caagtctttg
     3181 accaccctgg gaaaggtcat ctctgctctg gctgaaatgg actctggacc caacaagaac
     3241 aagaaaaaga agaagacaga tttcatccct taccgagact ccgtattgac atggcttctc
     3301 cgggaaaacc tcggtggcaa ctcaaggaca gccatggtgg cagccctgag ccctgcggac
     3361 atcaactatg atgagaccct cagcacactg agatatgcag atcgggccaa acaaatccgc
     3421 tgcaatgcca tcatcaatga agaccccaac aacaagctga ttagagaact gaaggatgag
     3481 gtgaccaggc tgagagacct gctgtatgcc caaggtcttg gcgatatcac tgacatgacc
     3541 aatgccctgg tgggcatgag cccctcatct tcactctcag ccctgtccag ccgtgcagcc
     3601 tctgtgtcca gtctccatga gcgcatcttg tttgctccag gcagcgagga ggctattgaa
     3661 aggctgaagg aaacagagaa gatcattgct gaactcaatg agacctggga ggagaagctg
     3721 cggcggacag aagccatccg gatggagagg gaagccttgc tggctgagat gggtgtggcc
     3781 atgagggaag atggtggtac cttgggtgtg ttttctccca aaaagactcc tcatcttgtc
     3841 aacctgaatg aggacccttt gatgtccgaa tgcctccttt actacatcaa agatggcgtc
     3901 accagagtgg gcagagaaga tgcagagagg cggcaagaca ttgtcctgag tggacacttc
     3961 atcaaagagg agcactgcat cttccggagt gactcccgag gaggcggaga agctgtggtg
     4021 accctggagc cctgtgaagg agcagacaca tatgtcaacg gcaagaaagt cactgagccc
     4081 agcatcctac ggtcaggaaa ccgcatcatc atgggaaaaa gccacgtgtt ccggtttaac
     4141 caccctgagc aggcccgtca ggagcgtgag cgaacacctt gtgcagagac acctgctgag
     4201 ccagtggact gggcctttgc ccagcgggaa ctgctggaga agcaaggcat tgacatgaag
     4261 caggagatgg agcagaggct ccaggagttg gaggaccagt accggaggga gcgggaggag
     4321 gccacatacc ttctggagca gcagaggctg gactatgaga gtaagctgga ggccctacag
     4381 aaacagatgg actccaggta ctacccagag gtgaacgagg aggaagaaga gccagaggat
     4441 gaagtccaat ggacagaacg agaatgtgag ctggcattgt gggccttccg gaagtggaag
     4501 tggtaccagt ttacttctct gcgggacctg ctgtggggca atgccatctt cctgaaggag
     4561 gccaacgcca tcagtgtgga gctaaagaag aaggtccagt tccagtttgt cctcctcacg
     4621 gacaccctgt actccccact gccgccagac ctactgcccc cagaagccgc caaagaccga
     4681 gagacacgac ccttccccag gaccattgtg gcagtggagg tccaggacca gaagaatggg
     4741 gccacccact actggacact ggagaaactc aggcaacgcc tggacctgat gcgggaaatg
     4801 tacgacaggg ctgcagaggt tccctccagc gtcgttgagg actgtgacaa cgtggtgacc
     4861 ggcggggacc ccttctatga ccgcttcccc tggttccgac tggtgggcag ggccttcgta
     4921 tacctgagca acctgctgta ccctgtgccc ctggtgcacc gagtggcaat cgtcagcgaa
     4981 aagggagagg tgaaaggctt cctccgtgtg gcggtgcagg ccatctcagc tgatgaagag
     5041 gctccggatt atggctcggg tgtccgtcag tcaggaactg ctaaaatctc ctttgatgac
     5101 cagcattttg aaaagttcca atctgagtcc tgccctgtgg tggggatgtc ccgctcagga
     5161 acttcccaag aggagctgcg cattgtagaa ggccagggtc agggggctga tgctgggcct
     5221 tcagctgatg aagttaacaa caacacctgt tcagctgtgc ctcctgaagg cctcatggat
     5281 agcccagaga aggctgccct ggatgggcct ctggacacag ccctggacca cctccgtcta
     5341 ggcagtacct tcaccttccg ggtgacagtc ctgcaggcgt ccagtatctc tgctgaatac
     5401 gctgacatct tctgccagtt caactttatc caccgccacg atgaggcctt ctccactgaa
     5461 cccctgaaga acactggcag aggaccccct cttggcttct accatgtcca aaatattgcg
     5521 gtagaggtga ccaagtcctt catcgaatac atcaagagcc agcccattgt gttcgaggtc
     5581 tttgggcact accagcagca cccgttccca cctctttgca aggatgtcct cagccccctg
     5641 agaccctcac gtcgccactt ccccagggtc atgccgttat ccaagccagt gcccgccact
     5701 aagctcagca cgatgacacg gccttcccca ggtccctgcc attgcaagta tgacctgctg
     5761 gtctacttcg agatctgcga gctggaagcc aatggcgatt acatccctgc tgtggtggac
     5821 caccgtggtg gcatgccgtg catggggact ttccttctcc atcagggcat ccagagacgg
     5881 ataactgtga cactgttaca tgagacagga agccacatcc gctggaagga agtgcgcgag
     5941 ctagttgtgg gtcgcatccg aaacacccca gaaactgacg aagctctgat tgaccccaac
     6001 atcttgtccc tcaacatcct ctcatcagga tacgtccatc cagcccagga tgaccggacc
     6061 ttctaccagt ttgaagcagc ctgggacagt tccatgcata actctctcct gctgaatcgt
     6121 gtcacccctt accgggagaa aatctacatg acgctctctg cctacattga gatggagaac
     6181 tgtacccagc tagctgtcat caccaaggac ttctgcatgg ttttctactc tcgggacgcc
     6241 aagctacccg cctcacgctc cattcgcaac ctgtttggca gtggcagcct gcgggccaca
     6301 gaaggcaacc gtgtgactgg tgtgtatgag ctcagccttt gccacgtggc cgacgcaggc
     6361 agcccaggga tgcagcgtcg gcgccggcgg gtgctggaca catcagtagc ctatgtccgg
     6421 ggtgaggaga acctggctgg ctggaggccc cgaagtgaca gcctgatcct agatcaccag
     6481 tgggaactgg agaaactgag cctcttacag gaggtggaaa agaccaggca ctacctgctc
     6541 cttcgggaga agctggagac gacccagcgc cctggccctg aggtgctgtc cccagcctcc
     6601 agtgaggact ctgagtcccg tagttcttca ggtgcctctt ccccattgtc agccgagggt
     6661 cagccgtcac ctctggaggc tcccaacgag aggcagaggg agctggctgt caagtgctta
     6721 cgactcctca tgcacacatt caacagggaa tacacccaca gccacgtctg tatcagcgcc
     6781 agcgagagca agctctctga gatgtctgtc accctgatgc gggacccatc catgtcccct
     6841 ctgggtgctg ctacactcac tccctcctcc acttgcccct ctctgatcga aggacgatac
     6901 ggtgccactg acgtgaggac cccccagccc tgctcccggc ccgccagccc agagcccgag
     6961 ctcctgccag aacttgactc taagaagact ccctcccctg ttcgggcaac agagacagag
     7021 aaggagcccc agcgactgct ggtccctgac atccaagaga tccgagtcag cccaatcgtc
     7081 tccaagaagg gatacctgca tttcctggag ccacacacgg ctggctgggc caagcgcttt
     7141 gtggtggtga ggcgtcccta cgcctacatg tacaacagtg acaaggacac ggtggagaga
     7201 tttgtgctca acctctccac tgcgcaggtg gagtacagcg aggaccagca ggccatgctc
     7261 aagacaccca acacattcgc tgtgtgcacg gagcaccgag gcatcctgct gcaggccaac
     7321 agtgacaagg acatgcacga ctggctgtat gccttcaacc cgctcctggc tgggaccatc
     7381 cggtccaagc tctctcgaag gagatctgcg cagatgcggg tctgagtcga cggtaccgcg
     7441 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     7501 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7561 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7681 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     7741 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7801 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7861 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7921 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7981 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     8041 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     8101 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     8161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8281 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8401 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8461 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8581 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8641 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     8701 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8761 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8821 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     8881 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8941 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     9001 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     9061 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     9121 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     9181 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9241 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     9301 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9361 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9421 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     9481 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     9541 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9601 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     9661 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     9721 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9781 ggctggcact ctgtcgat
//
