LOCUS       sequence-338425-        8788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8788)
  TITLE     Targeting plasmids for creation of endogenously EosHalo-tagged NuRD 
            component proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pChd4EosHaloTAF_Puro
FEATURES             Location/Qualifiers
     source          1..8788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2217..2233
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2218..2234
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2990..3664
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="SAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNARN
                     DITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             4556..4576
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4625..4690
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    4738..4762
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4800..4831
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        4881..5380
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4916..4939)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5313..5332
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5394..5403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5400..5999
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5899..5919
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6006..7001
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7023..7040)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7029..7253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7301..7332
                     /label=rox
                     /bound_moiety="Dre recombinase"
     protein_bind    complement(7350..7374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8147..8166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8148..8166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8173..8190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8173..8189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8182..8204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8330..8785)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8467..8488)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8679..8698
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 ggtcgacggt atcgataagc ttgatatcga attggtgtgg atgcagagag gagatggtag
     2281 gaactgagcc tctcaagtgg aggagcccaa ggctgagaat aaaaactctg tgggcatttg
     2341 ctgtgctccc aacttgggag cacttggagg cgggagcacg atgagatgtg ctcatgcttg
     2401 gctgcatagg aagtttgaag ccaacctttt atataaaaaa aaaaaaaaaa aaaaaaaaaa
     2461 agaaagaaag aaaaagaaaa tggagacaat agtgagaagg aataatgaac taaagtttgg
     2521 tggctcttaa aaacaagaat ccgccagctg tggatggccg atgcctagag tcacagggac
     2581 tcaagagaca cagcaggacc tgagctcaag tctcaggcca tcctggacaa catgtgagac
     2641 cagagccact tgcaactaga atgggaggaa tagctgggtg ggcaggccag gtaaagcgag
     2701 tgtcaagagc gtatcctgtg tatgccagcg tgacaaccag ccctgtgggc cacagctgtg
     2761 cccttgacta ccacagctca cggcctcttt ctttcttttt ccctctgcag tcctgaaaca
     2821 gctggaggaa ctgttgagtg acatgaaagc tgatgtgacg cggctcccag ccaccattgc
     2881 ccgcatcccc ccagtcgctg tgaggttaca gatgtcagag cggaacattc tcagccgcct
     2941 ggcaaacagg gcgccagaac ctcccccaca gcaggtagcc cagcagcaga gtgcgattaa
     3001 gccagacatg aagatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat
     3061 cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa
     3121 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
     3181 cagggtattc gccaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa
     3241 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca acgccagaaa
     3301 cgacataaca atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt
     3361 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
     3421 aatgtatgtg cgtgatggag tgctgacggg tgatattgag atggctttgt tgcttgaagg
     3481 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     3541 gttaccaggc gcccactttg tggaccactg cattgagatt ttaagccatg acaaagatta
     3601 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     3661 acgagccatg gcagaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     3721 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     3781 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     3841 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     3901 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     3961 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     4021 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     4081 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     4141 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     4201 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     4261 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     4321 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     4381 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     4441 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     4501 cccggacctg atcggcagcg agatcgcgcg ctggctgtcg acgctcgagg ctagcgagaa
     4561 cctgtacttc cagggaatgg ctggtggcct gaatgacatc tttgaggccc agaagatcga
     4621 gtgggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     4681 cgatgacaag tagaagcttc gatcggtacc aaggcgcata acgataccac gatatcaaca
     4741 agtttgtaca aaaaagcagg ctggcgccgg aaccaattca gtcgacgttt aaacgatcgt
     4801 aactttaaat aatgccaatt atttaaagtt acatatgaga aagtatagga acttcagctt
     4861 gatatcgaat taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     4921 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     4981 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     5041 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     5101 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     5161 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     5221 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     5281 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     5341 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aacgccacca
     5401 tggggaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     5461 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5521 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5581 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5641 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5701 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5761 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5821 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5881 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5941 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg
     6001 gatccatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg gggaaaacca
     6061 ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc
     6121 cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac
     6181 aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg
     6241 cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg
     6301 ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc
     6361 atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc
     6421 cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg
     6481 tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg
     6541 gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca
     6601 atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga cagctttcgg
     6661 ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata
     6721 tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc
     6781 tgtacaacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg
     6841 tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta
     6901 cctccgggat ggtccagacc cacgtcacca cccccggctc cataccgacg atctgcgacc
     6961 tggcgcgcac gtttgcccgg gagatggggg aggctaactg agctctagag ctcgctgatc
     7021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7261 ggcggaaaga accagctggg gctcgacata ctagagtact taactttaaa taatgccaat
     7321 tatttaaagt taggcgcgcc gatatctaga cccagctttc ttgtacaaag tggttgatat
     7381 ctctatagtc gcagtaggcg gaagacccag agtgatatcc acctccacca ctcagcagtg
     7441 accttcctca ctttctcttg tcccggctgc ccctgggggc cagagagacc cttgccttcc
     7501 ttctgcccat cttctgagtt gtaaaggaac agccctgcgc agtggggcag ggagggaggg
     7561 agaagcaatg gtgcccttcc tgctggagag acaagcagca gtagcgcagc gcccgcccgc
     7621 catgtgcagg agctggcggg ctggctgcct tctggaccct ggcttctccc caccgtgcgc
     7681 ctgttacaaa ctgttgtgga ttctgccagg ctcgaagaaa atgatctgaa tttcttcctc
     7741 tttttggttt tattttgttg gtttactttg tgttttctcc tttttggggg gtattcagag
     7801 tgggccgggc ccctgggcga gacacagcta cctctgttgg catcttttta ataccaggaa
     7861 cccagcggct ccagccactg agcagctaaa taaagttgga aaaaaaaaaa aaaagaaaaa
     7921 gaaccaaaag cataaaaaac cacagcaaat ttcttgatga gaactgaaaa taaaagtttc
     7981 cttgtatttt agtgatctgg ttctgtgtgg gtgagtgtgg gttccaggta ccccaggaac
     8041 tggcttatcc tggaagactg cctgtgctcc tgacagggtg acattctggc gaacaagagg
     8101 ctcactagtt ctagagcggc cgccaccgcg gtggagctcc aattcgccct atagtgagtc
     8161 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8221 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8281 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     8341 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     8401 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     8461 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     8521 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     8581 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     8641 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     8701 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     8761 cttaatgcgc cgctacaggg cgcgtcag
//
