LOCUS       sequence-338429-        4439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Robust and extremely thermostable polymerase with 5x higher fidelity
            than Taq. Ideal for GC-rich or looped sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4439)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-GBDpol
FEATURES             Location/Qualifiers
     source          1..4439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2462..2480)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2476..2523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2528..2544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2528..2544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(2611..3199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2691..2710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3390..4181,4182..4250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4013..4032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4251..4355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4371..4387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4388..4419
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat gatcattgac gctgactaca tcactgaaga
       61 tggcaaaccg atcatccgta tttttaaaaa agagaaaggt gaatttaaag tcgaatacga
      121 ccgcaccttt cgcccgtaca tttacgcgct gctgaaagat gacagcgcta ttgatgaagt
      181 gaagaaaatt actgcggaac gtcatggtaa aattgttcgt attacggagg tggaaaaagt
      241 ccaaaaaaaa ttcctgggcc gtcctatcga ggtgtggaag ctgtacctgg aacatcctca
      301 ggatgttccg gcaattcgtg aaaaaatccg tgaacacccg gccgtcgtag atatcttcga
      361 gtacgatatc ccgttcgcaa aacgttacct gattgataaa ggcctgacgc cgatggaggg
      421 caacgaagaa ctgacctttc tggcggtgga catcgaaact ctgtatcacg aaggcgaaga
      481 atttggtaaa ggccctatta tcatgatttc gtatgcggat gaggaaggag cgaaagtgat
      541 cacatggaaa agcattgacc tgccgtacgt tgaagtggtc agctcagaac gcgaaatgat
      601 taaacgcctc gttaaagtca ttcgtgaaaa agatccggac gtaatcatca cctacaatgg
      661 tgataacttc gactttccgt atcttctgaa acgtgcggaa aaattgggca ttaaactgcc
      721 gcttggtcgt gacaacagcg aaccgaaaat gcagcgcatg ggtgacagcc tggcggtgga
      781 aattaaaggt cgtatccact tcgacctgtt cccggcgatc cgtcgtacca tcaacctgcc
      841 gacgtatacc ctggaaacgg tttacgaagt tattttcggc aaatccaaag aaaaagttta
      901 cgctcacgaa atcgcggaag catgggaaac cggtaaaggc ctggaacgtg tggcaaagta
      961 cagcatggaa gatgcaaaag tgaccagcga actggggaaa gaatttttcc caatggaagc
     1021 gcaactggca cgcctggtgg gtcatccggt ctgggacgtg tctcgtagct cgactggcaa
     1081 cctggtggaa tggttcctgc tgactaaagc ttacgaacgc aacgagctcg caccgaacaa
     1141 accggatgaa cgtgaatacg aacgccgtct gcgtgagagc tacgaaggcg gttacgtgaa
     1201 cgaaccggaa aaaggcctgt gggagggtat cgtgagcctg gatttccgta gcctgtaccc
     1261 gtcgattatc atcactcata acgtatcccc agatacgctc aaccgtgaaa actgtaaaga
     1321 atatgacgtt gcgcctcagg tgggtcatcg cttctgcaag gattttccag gtttcatccc
     1381 gagcctgctg ggaaacctgc tggaggaacg tcaaaaaatc aaaaaacgca tgaaagagag
     1441 taaagacccg gtggaaaaaa agctgctgga ctaccgtcag cgtgctatta agatccttgc
     1501 gaactcttat tacggttatt acggctatgc aaaagcgcgt tggtactgca aagaatgcgc
     1561 tgaatccgtt actgcatggg gtcgtcagta catcgacctg gtacgtaggg aactggagag
     1621 ccgtggcttc aaagtactgt atatcgacac tgatggtctg tacgccacta tcccgggcgc
     1681 aaaacatgaa gagatcaaag agaaagccct gaaattcgtc gagtatataa actccaaact
     1741 cccgggcctg ctggaactgg aatatgaagg cttctacgcg cgcggcttct ttgtgaccaa
     1801 aaaaaaatac gctctgattg atgaggaagg taaaatagtt acccgcggcc tggaaatcgt
     1861 gcgccgtgac tggagcgaaa tcgccaaaga gactcaggca aaagttctgg aagcaatcct
     1921 gaagcacggc aacgttgacg aggcggtgaa aatcgtaaag gaagttaccg aaaagctgtc
     1981 gaagtatgag atcccaccag aaaaactggt tatttacgag caaatcaccc gcccgttatc
     2041 agaatacaaa gcaattggcc cgcatgtagc tgtggccaaa cgcctggccg cgaaaggcgt
     2101 aaaagttaaa ccgggtatgg taattgggta catcgtcctg aagggagatg gtccaatcag
     2161 caaacgtgcc attgctattg aagagtttga tccgaaaaaa cacaaatacg acgccgaata
     2221 ctatattgaa aaccaagttc tgccggccgt tgaacgtatc ctgcgcgcgt tcggctaccg
     2281 taaggaggat ctgcgctacc aaaaaaccaa acaggtgggt ctgggtgctt ggctgaaatt
     2341 ttgaagagct ttgagaccac ggagccctta ttgtaaccgc ttcctacgcg cgctaaacct
     2401 agggataaca gggtaatttg tcagtcgatt cagtcggccg ctgaggtgtc aatcgtcgga
     2461 gccgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     2521 ttgcatggtc atagctgttt cctgagagct tggcaggtga tgacacacat taacaaattt
     2581 cgtgaggagt ctccagaaga atgccattaa tttccatagg ctccgccccc ctgacgagca
     2641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacacg
     3181 cagaaaaaaa ggatctcaag aaggcctact attagcaaca acgatccttt gatcttttct
     3241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     3541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4321 tgtatttaga aaaataaaca aataggggtt ccgcgcacct gcaccagtca gtaaaacgac
     4381 ggccagtagt caaaagcctc cgaccggagg cttttgactt ggttcaggtg gagtgggag
//
