LOCUS       sequence-338455-       15352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ptpTALECD vector assembly, Destination vector;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, 
            Takanashi H, Tsutsumi N, Arimura SI
  TITLE     Targeted base editing in the plastid genome of Arabidopsis thaliana.
  JOURNAL   Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. 
            Epub 2021 Jul 1.
  PUBMED    34211131
REFERENCE   2  (bases 1 to 15352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00954-6"; journalName: "Nat Plants"; date: 
            "2021-07"; volume: "7"; issue: "7"; pages: "906-913"
COMMENT     Sequence Label: pDest_pK7WG2_pRPS5A_CTP OleGFP
FEATURES             Location/Qualifiers
     source          1..15352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(466..1173,1174..1176,1177..1179))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             complement(join(466..1173,1174..1176,1177..1179))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(505..526)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     854..873
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1113..1134
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    1192..1216
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3714..3838
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3879..4184)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3981..4000)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4526..5185)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5100..5119
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(5239..5245,5246..5263,5264..5269))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5294..5418)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7314..7338
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8669..9298
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9727..10800
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10866..11060
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11296..11318)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11404..11544
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11459..11478
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11559..11576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11730..12318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11810..11829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12564..13355)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13876..13900
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(13993..14245)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(14285..15079)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14396..14415)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     15006..15025
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(15113..15292)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cccttaattc tccgctcatg atcgataatt cgaggtcgca tgcctgcagg tcactggatt
       61 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
      121 aagggtttct tatatgctca acacatgagc gaaaccctat aagaacccta attcccttat
      181 ctgggaacta ctcacacatt attctggaga aaaatagaga gagatagatt tgtagagaga
      241 gactggtgat ttttgcggac tctagcatgg ccctcccggc cgccatggtc attgtttgcc
      301 tccctgctgc ggtttttcac cgaagttcat gccagtccag cgtttttgca gcagaaaagc
      361 cgccgacttc ggtttgcggt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
      421 gatttcacgg gttggggttt ctacaggacg taacatgccg ggttcgtaca gctcgtccat
      481 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      541 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      601 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      661 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      721 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      781 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      841 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      901 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      961 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     1021 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     1081 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     1141 caccaccccg gtgaacagct cctcgccctt gctcaccatc cgcgggatat caccactttg
     1201 tacaagaaag ctgggtcggc gcgcccaccc ttgagtagtg tgctggccac cacgagtacg
     1261 gtcacggtca tgttccccac cagtatgttg ctgtccgtag tactgagctc tgtctttcag
     1321 atcctgagct ttgcttccca acttcatcct tgcactgtcc aacttgtctg atccctgtgg
     1381 gtgctctccc gttgcgtacc tattcacaat caataggtat cataccaagc attttaccta
     1441 tatagatgat atacacagac acattcaagc tagctaggtc aaatttcgta tttagttaac
     1501 caaatgttcc ctagaatttg caatttctta ttgttacatt tgttattcga ccaaaaaaat
     1561 tgatccaaag ctactggctc aacacatgca tgcacatatt gcacaaaatt atgaagtaag
     1621 atgataaatg tgtgcttact tgtaaatcca agagaaaacg gttatagcgg caatgccaaa
     1681 ccctccagag gaaagaaaac cggtgatgag gagtgcaact gtgatgagag ccgggacaag
     1741 gattgggctg aagataacga gcagaggtgt tgcaacagtc aaagctatga cagttccaac
     1801 aagggtaagg ctggagagaa caaggaggga accaccagct gtgacagcag ttgcagcttt
     1861 agcaatctgc ctagacttgg agtagtcaga tcctcgtccg gacatctggt actggtctcg
     1921 gtctcggccc atcatcgggt actggtctct gccgatgata tcgtgatggg ttcctctagc
     1981 tgtatccgcc atttttttgt tcttgtttac tagagagaat gagattttga atgagagatt
     2041 gatcaaaaag atgtgtatat attttggaag aggtaggagt gtttgttaga tatattaaga
     2101 aaggagacga gaagtcacgt gtcaatggag acatcatgca attgtaggcc acgtgttaat
     2161 ggcgtcacca tgcagccgct gcatgtgtta acgagaggag tctattcaaa gacacgtttg
     2221 ttttttgcct agctcagaat gtggcgttgg catcgaaccg aaacataaaa cgcaaaacga
     2281 acctttgggc tcgtcagtat gggtttgggc cttcaataga ttccttctat acggcccaca
     2341 tatccactat tggcaacaaa attagaaaaa tttaggattt agttttaaaa tttgttccgc
     2401 gacattctag aacaatgaag aaaatggata caaataataa atctatctgc ctcttaaaag
     2461 ttccaaattt caccacgtca ctgctttgat aatgcaggtc ctcgtcgtct ctcctccgct
     2521 catcgccgcc gcatctctct ctaaacctct caattctctc tctaaagccg cactctcttt
     2581 ctccagagcc aaacccattt gtcctttccc tcaaacctct cgacgaccaa tctccgtata
     2641 caaatcccca atgaacaacc ttttcaacag actcggattc gggtctcgtc ctcaagccca
     2701 agctgaccca tcttccgccg caatcgccca aggacccgac gatgatgttc cgtcttcagg
     2761 tcagcaattc gcgcaatttg gcgccggttg tttctgggga gtggagctag cttaccagag
     2821 agttcctggt gtgaccaaga ccgaggttgg gtatagccat ggcatcgtgc acaatccgag
     2881 ttacgaggat gtctgtactg gcactacggg acataacgag gttgttagag ttcagtatga
     2941 tcccaaagag tgtagctttg agagcttgct tgatgttttc tggaatcgtc atgatccaac
     3001 caccttgaat cgtcaggtga ttgattgctt taaccctaaa aatctagacg ttatcagtat
     3061 cagatcgtaa tttgaatcaa tagagagctc tatgtgtgtt ctgcatttgg ggctgaattg
     3121 tttattagat aacaacgact agattcacct gtttagacga actctctgtc ccttagtctt
     3181 tagttctaca tacatttggt agtctcaagt aactattggc taagttgtta tgagctagtt
     3241 agaatctcag gccttgttga tatgaggatt cgtgtttgat tatgtggaag tgacattgcc
     3301 atttccattt tcgtatgcga tcttgtactt gagtctgttc ttgttcatgt tactatacct
     3361 acatacactt gaagatgaat ctagtgttgt tgttgtggtt cttcacttac taacttttat
     3421 gtgttgatct aagtagggtg ccggggatcc tctagagggc ccgacgtcgc atgcctgcag
     3481 gtcactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
     3541 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct
     3601 aattccctta tctgggaact actcacacat tattctggag aaaaatagag agagatagat
     3661 ttgtagagag agactggtga tttttgcgga ctctagcatg gccgcgggat atcaccactt
     3721 tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     3781 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt
     3841 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
     3901 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     3961 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
     4021 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
     4081 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
     4141 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
     4201 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
     4261 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
     4321 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
     4381 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
     4441 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
     4501 cttttagtaa gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt
     4561 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     4621 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     4681 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4741 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4801 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4861 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4921 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     4981 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     5041 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     5101 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     5161 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
     5221 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     5281 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     5341 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     5401 ttttttgtac aaacttgtga tatcactagc cttcgcggcg tagacggtaa ccggagaata
     5461 gaggcggcgg gagtagcaag aagaaaaccg gagcgacggc gaaaaagaag aacatggagt
     5521 gaaagggaag agaggagaaa gaggagtgaa gcttggattc agcttcagag acaagactag
     5581 ctgtgaatcc atggctgtgg tgagagaaac agagcgtgag ctcaaataca caagtacgaa
     5641 atcagagtta tgagagagga tttggagaaa gaagatcacc tgaagatcgt ttgcgtcgcg
     5701 cgaatgagag agacggcaga gaaaacgaga agctttaaga ggacgaacaa aaaccctaat
     5761 gtcactcctt ttatatagat gagaaattta gggtttgggc tctaatggag ctgtctacat
     5821 gggctttcat ttctttatcc agtaaagaac cggtttaaca ggttccctgg tttatacgtg
     5881 gttttagccg gttaacttac ggaatattat ttttatctta agttacacaa attacatcat
     5941 taatagtaaa acaaaatttt atggtgcaat ttaagttttt atgatgagtg aaattcgaat
     6001 aatatacaaa tattttactg tttaggtttc cctaaaccta cttttagata tgtaaattaa
     6061 acattttttt ttttttggtt agaaccaagt cagtgatcat agaactcaaa aagctggtgt
     6121 ttcaattggt acaagcattt ttattgcaag atataaaaat tcagaaggac atatccttga
     6181 tgtatgtaca tgaaggaacc gattttgact ccaagctaca aagaagaaga agaatgatat
     6241 acaaagaaaa ctgcatatcc tcaccatgaa attgaaagct agaggaaggg gaataacaga
     6301 tactctgcgt agaagagatt ccatataaac cgataatatc ctgagatagc ttcctgcaat
     6361 aatcaaatgt ttctacactc cgttagatat acactccatt gatgttccgt gataacgagt
     6421 ttatgtttgt acaaaagtat tttaccttgg tgtagtttgc tttttctagt acccatgtct
     6481 accaaagatc aacaacaaag atattagaga ttaatgaatt tggcagtgtc aaatgcagaa
     6541 actaaagcat atagttatga agttcttgtg cattatctca aaagtatgta agagatataa
     6601 gagtatctca taatgcttta cctggaaaat taagcctgaa gccaagatca catgtgcagg
     6661 aatcatcaag ctacctctaa aaacctgttc attgttaaag gataagaggg ttggtctttt
     6721 caaagctgca tactgaggaa agtaacaata tcgcaacaaa aataaattgg tggcgatagt
     6781 ttatcaagct tctacatgca gtaatggtgt tcgtgcttta attaaccgaa atagttaaga
     6841 cggaaataga tgaattcacc tgaggcatgt agaaagctag tgatatggct gaaacataat
     6901 ttactagcag aagtccagaa ccgaggaatg caatgtttct cactccaagt tttgttgcca
     6961 gggttgatat ttggaaccta ttcaagatca atcaaaggca tgattaacta aacatgcttg
     7021 aacaaacaca taaagacaat atatcaaacg gagctggtta aacattgtct ttgactgtca
     7081 aatggattga gccaaagagc ggaaagaaaa tggttatgcg tgcagttact tactttcgat
     7141 ctccttcaac atcaggaagg tcctttgtaa tagcaatgac cagtgcaaac agtgtcacaa
     7201 aagatgtgat gaacgccaca ggtgcactgc aaatagcgaa tcaaaagttg agagcttagc
     7261 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     7321 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     7381 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     7441 cctcgggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatccaaccc
     7501 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     7561 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     7621 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     7681 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     7741 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     7801 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     7861 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     7921 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     7981 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     8041 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     8101 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     8161 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     8221 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     8281 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     8341 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     8401 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     8461 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     8521 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     8581 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     8641 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     8701 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     8761 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     8821 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     8881 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     8941 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     9001 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     9061 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     9121 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     9181 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     9241 cgatgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     9301 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     9361 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     9421 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     9481 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     9541 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     9601 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     9661 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     9721 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     9781 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     9841 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     9901 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     9961 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
    10021 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
    10081 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
    10141 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
    10201 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
    10261 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
    10321 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
    10381 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
    10441 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
    10501 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
    10561 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
    10621 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
    10681 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
    10741 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
    10801 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
    10861 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
    10921 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
    10981 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
    11041 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
    11101 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
    11161 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
    11221 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
    11281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    11341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    11401 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
    11461 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    11521 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11581 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11641 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11701 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    12121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    12181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12361 aacgaaaact cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg
    12421 gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag
    12481 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
    12541 ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc gcctttcacg
    12601 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
    12661 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
    12721 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
    12781 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
    12841 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
    12901 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
    12961 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
    13021 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
    13081 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
    13141 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
    13201 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
    13261 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
    13321 tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt tttagggcga
    13381 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
    13441 cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa
    13501 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
    13561 ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac
    13621 tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac aatcaacatg
    13681 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
    13741 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
    13801 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
    13861 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
    13921 taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattga attatcagct
    13981 tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
    14041 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
    14101 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
    14161 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
    14221 tttattgcca aatgtttgaa cgatctgctt gactctagct agagtccgaa ccccagagtc
    14281 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
    14341 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
    14401 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
    14461 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccgtggg
    14521 tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac agttcggctg
    14581 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
    14641 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
    14701 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
    14761 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
    14821 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
    14881 gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc ttgacaaaaa
    14941 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
    15001 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
    15061 atccatcttg ttcaatcatg cctcgatcga gttgagagtg aatatgagac tctaattgga
    15121 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
    15181 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
    15241 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
    15301 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ct
//
