LOCUS       sequence-338464-        3369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 1/3 for assembly of site-specific recombination based 
            Registers. Composed of the reversed sequences of the TNos 
            terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     413..434
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    531..552
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      555..598
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             782..1441
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(848..867)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1462..1556
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1739..2327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2228..2247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2481..2498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2522..2579)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(2841..3093)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(3132..3369,1..14))
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(3174..3195)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(3330..3369,1..473,474..476,477..479))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
ORIGIN
        1 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
       61 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      121 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      181 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      241 ttcatgtggt cggggtagcg ggcgaagcac ttcaggccgt agccgaaggt ggtcacgagg
      301 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      361 taggtggcgt cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      421 tccagctcga ccaggatggg caccacccca gtgaacagct cttcaccctt gctcaccatt
      481 tcgactagaa tagtaaattg taatgttgtt tctatcgaga ccactcatcg ctctagaagc
      541 ggccgcgaat tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc
      601 tgcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      661 tcaggtggca ctcgagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
      721 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      781 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      841 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      901 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      961 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     1021 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1081 aacgttttca tcactctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1141 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1201 gaatatgttt ttcgtatcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1261 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1321 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1381 tgtcggcaga atgcttaatg aattacaaca gtactgcgac gagtggcagg gcggggcgta
     1441 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     1501 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     1561 gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta
     2521 atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac
     2581 tgcagcggcc gctactagtc gcgatgagtg gtctcaggag cgagtcggtc ccattattga
     2641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2761 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2821 gaattagctt ggcaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
     2881 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     2941 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     3001 aacgtaattc aacagaaatt atatgataat cgtcgcaaga ccggcaacag gattcaatct
     3061 taagaaactt tattgccaaa tgtttgaacg atcgggatcg aagatccatt ccaagctcag
     3121 ctcatgactg acttgtagag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     3181 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag
     3241 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     3301 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     3361 ttgatgccg
//
