LOCUS       sequence-338482-        1301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant fusion protein gene combining IgG-Fc binding domain Z (3
            repeats) of Staphylococcal Protein A and bioluminescence reporter 
            Nanoluciferase in pET28a bacterial expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Nandy S, Crum M, Wasden K, Strych U, Goyal A, Maranholkar V, Mo W, 
            Vu B, Kourentzi K, Willson RC
  TITLE     Protein A-nanoluciferase fusion protein for generalized, sensitive 
            detection of immunoglobulin G.
  JOURNAL   Anal Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929.
  PUBMED    36270332
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929."
COMMENT     Sequence Label: pSNRW-Z3-Nluc
FEATURES             Location/Qualifiers
     source          1..1301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1213..1230
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1283..1301)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gtcgataaca aattcaacaa agaacaacaa
      181 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
      241 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
      301 ctaaatgatg ctcaagcacc gaaagtcgat aacaaattca acaaagaaca acaaaacgcg
      361 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
      421 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
      481 gatgctcaag caccgaaagt cgataacaaa ttcaacaaag aacaacaaaa cgcgttctat
      541 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
      601 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
      661 caagcaccga aagtcgactt acaagctggc atggttttca ccctggaaga cttcgttggt
      721 gattggcgtc agaccgcagg ttacaatctg gatcaggtcc tggaacaggg cggtgtatct
      781 agcctgttcc agaacctggg tgttagcgtc accccgatcc agcgtatcgt actgagcggt
      841 gaaaacggcc tgaaaatcga catccatgtt atcatcccgt acgagggtct gtctggtgac
      901 cagatgggtc agatcgaaaa gattttcaaa gtagtgtatc cggttgatga tcaccatttc
      961 aaagttatcc tgcactacgg caccctggta atcgacggtg tgacccctaa catgattgac
     1021 tacttcggtc gtccttacga aggcatcgca gttttcgatg gcaagaaaat caccgtaacc
     1081 ggtaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaatcc ggacggctct
     1141 ctgctgttcc gtgtcactat caacggcgtt actggctggc gtctgtgcga gcgtatcctg
     1201 gcgtaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
     1261 gaagctgagt tggctgctgc caccgctgag caataactag c
//
