LOCUS       sequence-338483-        1649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant fusion protein gene combining IgG-Fc binding domain Z (5
            repeats) of Staphylococcal Protein A and bioluminescence reporter 
            Nanoluciferase in pET28a bacterial expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Nandy S, Crum M, Wasden K, Strych U, Goyal A, Maranholkar V, Mo W, 
            Vu B, Kourentzi K, Willson RC
  TITLE     Protein A-nanoluciferase fusion protein for generalized, sensitive 
            detection of immunoglobulin G.
  JOURNAL   Anal Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929.
  PUBMED    36270332
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929."
COMMENT     Sequence Label: pSNRW-Z5-Nluc
FEATURES             Location/Qualifiers
     source          1..1649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1561..1578
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1631..1649)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gtcgataaca aattcaacaa agaacaacaa
      181 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
      241 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
      301 ctaaatgatg ctcaagcacc gaaagtcgat aacaaattca acaaagaaca acaaaacgcg
      361 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
      421 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
      481 gatgctcaag caccgaaagt cgataacaaa ttcaacaaag aacaacaaaa cgcgttctat
      541 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
      601 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
      661 caagcaccga aagtcgataa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc
      721 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
      781 gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga tgctcaagca
      841 ccgaaagtcg ataacaaatt caacaaagaa caacaaaacg cgttctatga gatcttacat
      901 ttacctaact taaacgaaga acaacgaaac gccttcatcc aaagtttaaa agatgaccca
      961 agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca agcaccgaaa
     1021 gtcgacttac aagctggcat ggttttcacc ctggaagact tcgttggtga ttggcgtcag
     1081 accgcaggtt acaatctgga tcaggtcctg gaacagggcg gtgtatctag cctgttccag
     1141 aacctgggtg ttagcgtcac cccgatccag cgtatcgtac tgagcggtga aaacggcctg
     1201 aaaatcgaca tccatgttat catcccgtac gagggtctgt ctggtgacca gatgggtcag
     1261 atcgaaaaga ttttcaaagt agtgtatccg gttgatgatc accatttcaa agttatcctg
     1321 cactacggca ccctggtaat cgacggtgtg acccctaaca tgattgacta cttcggtcgt
     1381 ccttacgaag gcatcgcagt tttcgatggc aagaaaatca ccgtaaccgg taccctgtgg
     1441 aacggcaaca aaattatcga cgagcgcctg atcaatccgg acggctctct gctgttccgt
     1501 gtcactatca acggcgttac tggctggcgt ctgtgcgagc gtatcctggc gtaactcgag
     1561 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     1621 gctgctgcca ccgctgagca ataactagc
//
