LOCUS       sequence-338485-        3396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of ALC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET41sIII_GST-ALC1
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             673..696
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ggttcaacta gtctggaagt tctgttccag gggcccctcg agatggagcg cgcgggcgct
      721 actagccgcg ggggccaggc gcctggcttc ttactgcggc ttcatactga gggcccagcc
      781 gaggcggcgc gggtgcagga gcaggactta cggcagtggg ggctgacagg gattcaccta
      841 cgctcttacc agctggaggg agtaaactgg ctcgcccagc gcttccattg tcagaatggc
      901 tgtatcctgg gagatgagat gggcctgggg aagacctgcc agactattgc tctcttcatt
      961 tatttggcag gaagattaaa tgatgaaggg ccatttctga ttctttgtcc cttgtctgtt
     1021 ttgagcaact ggaaagaaga aatgcagaga tttgctccag gtctttcctg tgtaacatat
     1081 gcaggcgaca aggaggaaag agcctgcctt cagcaagacc tgaaacagga gtcacgtttt
     1141 catgtgctac tgactaccta tgagatttgc ttgaaagatg catcatttct aaaatcattc
     1201 ccttggagtg ttcttgttgt ggatgaagct cacaggttga aaaaccaaag ctccctgctg
     1261 cataagacct tgtcagagtt ctcagtagtc ttcagtctcc tgttgaccgg aactcccatc
     1321 cagaacagcc tccaagagct ctactccctc ctcagttttg tggagcctga tctcttttcc
     1381 aaggaagagg tgggagattt tattcaacgc taccaggata ttgagaaaga atctgagtca
     1441 gcaagtgaac tgcacaaact cttgcagcca tttctgctga ggcgagtgaa agctgaggta
     1501 gctacagagc ttcccaagaa gacagaagta gtgatatacc atggcatgtc agcattgcag
     1561 aagaaatact acaaggccat tttgatgaaa gacctagatg catttgaaaa tgagacggca
     1621 aagaaagtta aactacagaa cattttgtcc cagcttcgaa agtgtgtgga tcacccatat
     1681 ttgtttgatg gtgtggagcc ggagcctttt gaagttggag accacctgac tgaggctagt
     1741 gggaagcttc acctgctgga taagctacta gcattcctgt attctggggg ccatcgggtt
     1801 ttacttttct cccaaatgac ccagatgttg gatattctcc aagactatat ggattacaga
     1861 ggctacagct atgagcgtgt ggatggttct gtgagaggag aagagagaca cttggccatt
     1921 aagaactttg gacagcagcc cattttcgtt tttctcctga gtactagggc aggtggagtt
     1981 ggcatgaact taacagcagc agatactgtg atttttgttg acagtgactt taatcctcag
     2041 aatgacttgc aagcagctgc cagggctcat cgcattggcc aaaacaagtc tgttaaagtt
     2101 attcggctga ttggtcgaga cactgtggaa gaaatagtct ataggaaagc agcctccaaa
     2161 ctgcagctca ccaacatgat catagaagga ggccatttta ctctgggagc ccaaaaaccc
     2221 gctgccgatg ctgacctcca gttgagtgag atactcaaat ttggtttgga taaactgctg
     2281 gcctctgagg ggagcaccat ggatgaaata gacctggagt ccatcctggg agaaacaaaa
     2341 gatggccagt gggtctctga tgccttgcct gcagcagaag gagggagcag agatcaagag
     2401 gaaggaaaaa atcatatgta cttatttgaa ggtaaagatt attctaaaga gcccagtaag
     2461 gaagacagaa aatcatttga acaactggta aaccttcaga aaaccctttt ggagaaagct
     2521 agtcaagagg gccgatcact ccgaaataaa ggcagtgttc tcatcccagg ccttgtggag
     2581 ggatctacca aaaggaagcg ggttctgagt ccagaagagc tggaggacag acagaagaaa
     2641 agacaagaag cagctgccaa gagaaggaga ctcatagagg agaagaagag gcaaaaggaa
     2701 gaggctgaac ataagaaaaa gatggcctgg tgggaatcca acaattacca gtccttctgc
     2761 ctgccctctg aggagagcga gccagaggac cttgagaatg gggaagagag ctctgctgag
     2821 ctggattacc aagacccaga tgctacttcc ctcaagtacg ttagtggtga tgtcacccac
     2881 cctcaggctg gggccgagga tgctctcatt gtgcactgcg tagatgactg tggccactgg
     2941 ggcagaggtg gtttatttac agctctggaa aagcgatccg ctgagccaag aaaaatatat
     3001 gagctggctg ggaaaatgaa agacctgagt ttgggaggtg tccttttatt tcctgttgat
     3061 gataaagaat caagaaacaa agggcaagat ttgttggcct tgattgtggc tcagcatcgt
     3121 gatcgttcca atgtcctgtc tggcattaag atggcagccc tagaagaggg cctgaagaag
     3181 atatttttag cagcaaaaaa gaagaaagca agtgttcatc ttccacgtat tggacatgcc
     3241 acgaaaggtt ttaactggta tggtactgag cgacttattc ggaaacatct ggctgcaaga
     3301 ggcatcccaa cttacatata ttattttcct agaagcaagt ctgctgtcct tcattcacag
     3361 tcttcatctt cctcctcaag acagctggtg ccttaa
//
