LOCUS       sequence-338489-        2757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of PARP1 lacking Zn2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1delZn2
FEATURES             Location/Qualifiers
     source          1..2757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgatggcgg agtcttctga taagctgtat cgagtcgagt acgccaagag cgggcgcgcc
      121 tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc catcatggtg
      181 cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg cttctggaag
      241 gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga gcttcggtgg
      301 gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg cgatggagtg
      361 gatgaagtgg cgaagaagaa atctaaaaaa gaaaaagaca aggatagtaa gcttgaaaaa
      421 gccctaaagg ctcagaacga cctgatctgg aacatcaagg acgagctaaa gaaagtgtgt
      481 tcaactaatg acctgaagga gctactcatc ttcaacaagc agcaagtgcc ttctggggag
      541 tcggcgatct tggaccgagt agctgatggc atggtgttcg gtgccctcct tccctgcgag
      601 gaatgctcgg gtcagctggt cttcaagagc gatgcctatt actgcactgg ggacgtcact
      661 gcctggacca agtgtatggt caagacacag acacccaacc ggaaggagtg ggtaacccca
      721 aaggaattcc gagaaatctc ttacctcaag aaattgaagg ttaaaaagca ggaccgtata
      781 ttccccccag aaaccagcgc ctccgtggcg gccacgcctc cgccctccac agcctcggct
      841 cctgctgctg tgaactcctc tgcttcagca gataagccat tatccaacat gaagatcctg
      901 actctcggga agctgtcccg gaacaaggat gaagtgaagg ccatgattga gaaactcggg
      961 gggaagttga cggggacggc caacaaggct tccctgtgca tcagcaccaa aaaggaggtg
     1021 gaaaagatga ataagaagat ggaggaagta aaggaagcca acatccgagt tgtgtctgag
     1081 gacttcctcc aggacgtctc cgcctccacc aagagccttc aggagttgtt cttagcgcac
     1141 atcttgtccc cttggggggc agaggtgaag gcagagcctg ttgaagttgt ggccccaaga
     1201 gggaagtcag gggctgcgct ctccaaaaaa agcaagggcc aggtcaagga ggaaggtatc
     1261 aacaaatctg aaaagagaat gaaattaact cttaaaggag gagcagctgt ggatcctgat
     1321 tctggactgg aacactctgc gcatgtcctg gagaaaggtg ggaaggtctt cagtgccacc
     1381 cttggcctgg tggacatcgt taaaggaacc aactcctact acaagctcca gcttctggag
     1441 gacgacaagg aaaacaggta ttggatattc aggtcctggg gccgtgtggg tacggtgatc
     1501 ggtagcaaca aactggaaca gatgccgtcc aaggaggatg ccattgagca cttcatgaaa
     1561 ttatatgaag aaaaaaccgg gaacgcttgg cactccaaaa atttcacgaa gtatcccaaa
     1621 aagttctacc ccctggagat tgactatggc caggatgaag aggcagtgaa gaagctgaca
     1681 gtaaatcctg gcaccaagtc caagctcccc aagccagttc aggacctcat caagatgatc
     1741 tttgatgtgg aaagtatgaa gaaagccatg gtggagtatg agatcgacct tcagaagatg
     1801 cccttgggga agctgagcaa aaggcagatc caggccgcat actccatcct cagtgaggtc
     1861 cagcaggcgg tgtctcaggg cagcagcgac tctcagatcc tggatctctc aaatcgcttt
     1921 tacaccctga tcccccacga ctttgggatg aagaagcctc cgctcctgaa caatgcagac
     1981 agtgtgcagg ccaaggcgga aatgcttgac aacctgctgg acatcgaggt ggcctacagt
     2041 ctgctcaggg gagggtctga tgatagcagc aaggatccca tcgatgtcaa ctatgagaag
     2101 ctcaaaactg acattaaggt ggttgacaga gattctgaag aagccgagat catcaggaag
     2161 tatgttaaga acactcatgc aaccacacac aatgcgtatg acttggaagt catcgatatc
     2221 tttaagatag agcgtgaagg cgaatgccag cgttacaagc cctttaagca gcttcataac
     2281 cgaagattgc tgtggcacgg gtccaggacc accaactttg ctgggatcct gtcccagggt
     2341 cttcggatag ccccgcctga agcgcccgtg acaggctaca tgtttggtaa agggatctat
     2401 ttcgctgaca tggtctccaa gagtgccaac tactgccata cgtctcaggg agacccaata
     2461 ggcttaatcc tgttgggaga agttgccctt ggaaacatgt atgaactgaa gcacgcttca
     2521 catatcagca agttacccaa gggcaagcac agtgtcaaag gtttgggcaa aactacccct
     2581 gatccttcag ctaacattag tctggatggt gtagacgttc ctcttgggac cgggatttca
     2641 tctggtgtga atgacacctc tctactatat aacgagtaca ttgtctatga tattgctcag
     2701 gtaaatctga agtatctgct gaaactgaaa ttcaatttta agacctccct gtggtaa
//
