LOCUS       sequence-338495-        2667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of PARP1 lacking BRCT domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1delBRCT
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgatggcgg agtcttctga taagctgtat cgagtcgagt acgccaagag cgggcgcgcc
      121 tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc catcatggtg
      181 cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg cttctggaag
      241 gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga gcttcggtgg
      301 gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg caaaggccag
      361 gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga gtatgccaag
      421 tccaacagaa gtacgtgcaa ggggtgtatg gagaagatag aaaagggcca ggtgcgcctg
      481 tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg ctggtaccat
      541 ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta cagtgcgagt
      601 cagctcaagg gcttcagcct ccttgctaca gaggataagg aagccctgaa gaagcagctc
      661 ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt ggatgaagtg
      721 gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa agccctaaag
      781 gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg ttcaactaat
      841 gacctgaagg agctactcat cttcaacaag cagcaagtgc cttctgggga gtcggcgatc
      901 ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga ggaatgctcg
      961 ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac tgcctggacc
     1021 aagtgtatgg tcaagacaca gacacccaac cggaaggagt gggtaacccc aaaggaattc
     1081 cgagaaatct cttacctcaa gaaattgaag gttaaaaagc aggaccgtat attcccccca
     1141 gaaaccagcg cctccgtggc ggccacgcct ccgccctcca caaagagaat gaaattaact
     1201 cttaaaggag gagcagctgt ggatcctgat tctggactgg aacactctgc gcatgtcctg
     1261 gagaaaggtg ggaaggtctt cagtgccacc cttggcctgg tggacatcgt taaaggaacc
     1321 aactcctact acaagctcca gcttctggag gacgacaagg aaaacaggta ttggatattc
     1381 aggtcctggg gccgtgtggg tacggtgatc ggtagcaaca aactggaaca gatgccgtcc
     1441 aaggaggatg ccattgagca cttcatgaaa ttatatgaag aaaaaaccgg gaacgcttgg
     1501 cactccaaaa atttcacgaa gtatcccaaa aagttctacc ccctggagat tgactatggc
     1561 caggatgaag aggcagtgaa gaagctgaca gtaaatcctg gcaccaagtc caagctcccc
     1621 aagccagttc aggacctcat caagatgatc tttgatgtgg aaagtatgaa gaaagccatg
     1681 gtggagtatg agatcgacct tcagaagatg cccttgggga agctgagcaa aaggcagatc
     1741 caggccgcat actccatcct cagtgaggtc cagcaggcgg tgtctcaggg cagcagcgac
     1801 tctcagatcc tggatctctc aaatcgcttt tacaccctga tcccccacga ctttgggatg
     1861 aagaagcctc cgctcctgaa caatgcagac agtgtgcagg ccaaggcgga aatgcttgac
     1921 aacctgctgg acatcgaggt ggcctacagt ctgctcaggg gagggtctga tgatagcagc
     1981 aaggatccca tcgatgtcaa ctatgagaag ctcaaaactg acattaaggt ggttgacaga
     2041 gattctgaag aagccgagat catcaggaag tatgttaaga acactcatgc aaccacacac
     2101 aatgcgtatg acttggaagt catcgatatc tttaagatag agcgtgaagg cgaatgccag
     2161 cgttacaagc cctttaagca gcttcataac cgaagattgc tgtggcacgg gtccaggacc
     2221 accaactttg ctgggatcct gtcccagggt cttcggatag ccccgcctga agcgcccgtg
     2281 acaggctaca tgtttggtaa agggatctat ttcgctgaca tggtctccaa gagtgccaac
     2341 tactgccata cgtctcaggg agacccaata ggcttaatcc tgttgggaga agttgccctt
     2401 ggaaacatgt atgaactgaa gcacgcttca catatcagca agttacccaa gggcaagcac
     2461 agtgtcaaag gtttgggcaa aactacccct gatccttcag ctaacattag tctggatggt
     2521 gtagacgttc ctcttgggac cgggatttca tctggtgtga atgacacctc tctactatat
     2581 aacgagtaca ttgtctatga tattgctcag gtaaatctga agtatctgct gaaactgaaa
     2641 ttcaatttta agacctccct gtggtaa
//
