LOCUS       sequence-338496-        2316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of PARP1 lacking Zn2 and BRCT domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1delZn2delBRCT
FEATURES             Location/Qualifiers
     source          1..2316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgatggcgg agtcttctga taagctgtat cgagtcgagt acgccaagag cgggcgcgcc
      121 tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc catcatggtg
      181 cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg cttctggaag
      241 gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga gcttcggtgg
      301 gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg cgatggagtg
      361 gatgaagtgg cgaagaagaa atctaaaaaa gaaaaagaca aggatagtaa gcttgaaaaa
      421 gccctaaagg ctcagaacga cctgatctgg aacatcaagg acgagctaaa gaaagtgtgt
      481 tcaactaatg acctgaagga gctactcatc ttcaacaagc agcaagtgcc ttctggggag
      541 tcggcgatct tggaccgagt agctgatggc atggtgttcg gtgccctcct tccctgcgag
      601 gaatgctcgg gtcagctggt cttcaagagc gatgcctatt actgcactgg ggacgtcact
      661 gcctggacca agtgtatggt caagacacag acacccaacc ggaaggagtg ggtaacccca
      721 aaggaattcc gagaaatctc ttacctcaag aaattgaagg ttaaaaagca ggaccgtata
      781 ttccccccag aaaccagcgc ctccgtggcg gccacgcctc cgccctccac aaagagaatg
      841 aaattaactc ttaaaggagg agcagctgtg gatcctgatt ctggactgga acactctgcg
      901 catgtcctgg agaaaggtgg gaaggtcttc agtgccaccc ttggcctggt ggacatcgtt
      961 aaaggaacca actcctacta caagctccag cttctggagg acgacaagga aaacaggtat
     1021 tggatattca ggtcctgggg ccgtgtgggt acggtgatcg gtagcaacaa actggaacag
     1081 atgccgtcca aggaggatgc cattgagcac ttcatgaaat tatatgaaga aaaaaccggg
     1141 aacgcttggc actccaaaaa tttcacgaag tatcccaaaa agttctaccc cctggagatt
     1201 gactatggcc aggatgaaga ggcagtgaag aagctgacag taaatcctgg caccaagtcc
     1261 aagctcccca agccagttca ggacctcatc aagatgatct ttgatgtgga aagtatgaag
     1321 aaagccatgg tggagtatga gatcgacctt cagaagatgc ccttggggaa gctgagcaaa
     1381 aggcagatcc aggccgcata ctccatcctc agtgaggtcc agcaggcggt gtctcagggc
     1441 agcagcgact ctcagatcct ggatctctca aatcgctttt acaccctgat cccccacgac
     1501 tttgggatga agaagcctcc gctcctgaac aatgcagaca gtgtgcaggc caaggcggaa
     1561 atgcttgaca acctgctgga catcgaggtg gcctacagtc tgctcagggg agggtctgat
     1621 gatagcagca aggatcccat cgatgtcaac tatgagaagc tcaaaactga cattaaggtg
     1681 gttgacagag attctgaaga agccgagatc atcaggaagt atgttaagaa cactcatgca
     1741 accacacaca atgcgtatga cttggaagtc atcgatatct ttaagataga gcgtgaaggc
     1801 gaatgccagc gttacaagcc ctttaagcag cttcataacc gaagattgct gtggcacggg
     1861 tccaggacca ccaactttgc tgggatcctg tcccagggtc ttcggatagc cccgcctgaa
     1921 gcgcccgtga caggctacat gtttggtaaa gggatctatt tcgctgacat ggtctccaag
     1981 agtgccaact actgccatac gtctcaggga gacccaatag gcttaatcct gttgggagaa
     2041 gttgcccttg gaaacatgta tgaactgaag cacgcttcac atatcagcaa gttacccaag
     2101 ggcaagcaca gtgtcaaagg tttgggcaaa actacccctg atccttcagc taacattagt
     2161 ctggatggtg tagacgttcc tcttgggacc gggatttcat ctggtgtgaa tgacacctct
     2221 ctactatata acgagtacat tgtctatgat attgctcagg taaatctgaa gtatctgctg
     2281 aaactgaaat tcaattttaa gacctccctg tggtaa
//
