LOCUS       sequence-338499-        2688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of PARP1 lacking WGR domain but containing 
            L713F gain-of-function mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1delWGR-L713F
FEATURES             Location/Qualifiers
     source          1..2688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgatggcgg agtcttctga taagctgtat cgagtcgagt acgccaagag cgggcgcgcc
      121 tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc catcatggtg
      181 cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg cttctggaag
      241 gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga gcttcggtgg
      301 gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg caaaggccag
      361 gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga gtatgccaag
      421 tccaacagaa gtacgtgcaa ggggtgtatg gagaagatag aaaagggcca ggtgcgcctg
      481 tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg ctggtaccat
      541 ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta cagtgcgagt
      601 cagctcaagg gcttcagcct ccttgctaca gaggataagg aagccctgaa gaagcagctc
      661 ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt ggatgaagtg
      721 gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa agccctaaag
      781 gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg ttcaactaat
      841 gacctgaagg agctactcat cttcaacaag cagcaagtgc cttctgggga gtcggcgatc
      901 ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga ggaatgctcg
      961 ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac tgcctggacc
     1021 aagtgtatgg tcaagacaca gacacccaac cggaaggagt gggtaacccc aaaggaattc
     1081 cgagaaatct cttacctcaa gaaattgaag gttaaaaagc aggaccgtat attcccccca
     1141 gaaaccagcg cctccgtggc ggccacgcct ccgccctcca cagcctcggc tcctgctgct
     1201 gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct gactctcggg
     1261 aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg ggggaagttg
     1321 acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt ggaaaagatg
     1381 aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga ggacttcctc
     1441 caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca catcttgtcc
     1501 ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggccccaag agggaagtca
     1561 ggggctgcgc tctccaaaaa aagcaagggc caggtcaagg aggaaggtat caacaaatct
     1621 gaaaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat ctttgatgtg
     1681 gaaagtatga agaaagccat ggtggagtat gagatcgacc ttcagaagat gcccttgggg
     1741 aagctgagca aaaggcagat ccaggccgca tactccatct tcagtgaggt ccagcaggcg
     1801 gtgtctcagg gcagcagcga ctctcagatc ctggatctct caaatcgctt ttacaccctg
     1861 atcccccacg actttgggat gaagaagcct ccgctcctga acaatgcaga cagtgtgcag
     1921 gccaaggcgg aaatgcttga caacctgctg gacatcgagg tggcctacag tctgctcagg
     1981 ggagggtctg atgatagcag caaggatccc atcgatgtca actatgagaa gctcaaaact
     2041 gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa gtatgttaag
     2101 aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat ctttaagata
     2161 gagcgtgaag gcgaatgcca gcgttacaag ccctttaagc agcttcataa ccgaagattg
     2221 ctgtggcacg ggtccaggac caccaacttt gctgggatcc tgtcccaggg tcttcggata
     2281 gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta tttcgctgac
     2341 atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat aggcttaatc
     2401 ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc acatatcagc
     2461 aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc tgatccttca
     2521 gctaacatta gtctggatgg tgtagacgtt cctcttggga ccgggatttc atctggtgtg
     2581 aatgacacct ctctactata taacgagtac attgtctatg atattgctca ggtaaatctg
     2641 aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtaa
//
