LOCUS       sequence-338503-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of isolated PARP1 CAT domain containing L713F 
            gain-of-function mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1-CAT-L713F
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat ctttgatgtg
      121 gaaagtatga agaaagccat ggtggagtat gagatcgacc ttcagaagat gcccttgggg
      181 aagctgagca aaaggcagat ccaggccgca tactccatct tcagtgaggt ccagcaggcg
      241 gtgtctcagg gcagcagcga ctctcagatc ctggatctct caaatcgctt ttacaccctg
      301 atcccccacg actttgggat gaagaagcct ccgctcctga acaatgcaga cagtgtgcag
      361 gccaaggcgg aaatgcttga caacctgctg gacatcgagg tggcctacag tctgctcagg
      421 ggagggtctg atgatagcag caaggatccc atcgatgtca actatgagaa gctcaaaact
      481 gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa gtatgttaag
      541 aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat ctttaagata
      601 gagcgtgaag gcgaatgcca gcgttacaag ccctttaagc agcttcataa ccgaagattg
      661 ctgtggcacg ggtccaggac caccaacttt gctgggatcc tgtcccaggg tcttcggata
      721 gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta tttcgctgac
      781 atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat aggcttaatc
      841 ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc acatatcagc
      901 aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc tgatccttca
      961 gctaacatta gtctggatgg tgtagacgtt cctcttggga ccgggatttc atctggtgtg
     1021 aatgacacct ctctactata taacgagtac attgtctatg atattgctca ggtaaatctg
     1081 aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtaa
//
