LOCUS       sequence-338506-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express P53 DNA binding domain (94-312) in E.coli without tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Butler JS, Loh SN
  TITLE     Structure, function, and aggregation of the zinc-free form of the 
            p53 DNA binding domain.
  JOURNAL   Biochemistry. 2003 Mar 4;42(8):2396-403. doi: 10.1021/bi026635n.
  PUBMED    12600206
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2003-03-4- 4"; volume: "42"; issue: "8"; 
            pages: "2396-403"
COMMENT     Sequence Label: pET23 P53DBD
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             711..728
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(781..799)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      795..842
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttaagaagg agatatacat atgtcatctt ctgtcccttc ccagaaaacc taccagggca
       61 gctacggttt ccgtctgggc ttcttgcatt ctgggacagc caagtctgtg acttgcacgt
      121 actcccctgc cctcaacaag atgttttgcc aactggccaa gacctgccct gtgcagctgt
      181 gggttgattc cacacccccg cccggcaccc gcgtccgcgc catggccatc tacaagcagt
      241 cacagcacat gacggaggtt gtgaggcgct gcccccacca tgagcgctgc tcagatagcg
      301 atggtctggc ccctcctcag catcttatcc gagtggaagg aaatttgcgt gtggagtatt
      361 tggatgacag aaacactttt cgacatagtg tggtggtgcc ctatgagccg cctgaggttg
      421 gctctgactg taccaccatc cactacaact acatgtgtaa cagttcctgc atgggcggca
      481 tgaaccggag gcccatcctc accatcatca cactggaaga ctccagtggt aatctactgg
      541 gacggaacag ctttgaggtg cgtgtttgtg cctgtcctgg gagagaccgg cgcacagagg
      601 aagagaatct ccgcaagaaa ggggagcctc accacgagct gcccccaggg agcactaagc
      661 gagcactgcc caacaacacc taagtcgaca agcttgcggc cgcactcgag caccaccacc
      721 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      781 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      841 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      901 aagcgcggcg ggtgt
//
