LOCUS       sequence-338519-        2254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Full-length P53 in E.coli with cleavable 
            expression/purification tag (ribose binding protein circular 
            permutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ha JH, Yu X, Carpizo DR, Loh SN
  TITLE     Urea Denaturation, Zinc Binding, and DNA Binding Assays of Mutant 
            p53 DNA-binding Domains and Full-length Proteins.
  JOURNAL   Bio Protoc. 2021 Oct 20;11(20):e4188. doi: 10.21769/BioProtoc.4188. 
            eCollection 2021 Oct 20.
  PUBMED    34786438
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2021-10-20"; volume: "11(20):e4188. doi"; pages: " 
            10.21769/BioProtoc.4188. eCollection 2021 Oct 20"
COMMENT     Sequence Label: pKan cpRBP-FL P53
FEATURES             Location/Qualifiers
     source          1..2254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             644..667
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             680..1858
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     CDS             1874..1897
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2228..2251
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aggagatata catatgggta gctcacacca tcaccatcac cattcgagct cgggcgacgt
       61 agtgtcgcat atcgcaagcg ataacgtcaa gggaggtgag atggctgccg agttcattgc
      121 gaaagcctta aagggcaaag ggaacgtcgt ggaattagag ggcatcccag gagcgtcggc
      181 agctcgtgac cgcggaaaag ggtttgacga ggctattgct aagtacccag acatcaagat
      241 cgtggccaaa caagccgccg actttgatcg cagtaagggt ttgagcgtta tggaaaacat
      301 cttacaggcg caaccgaaaa ttgatgcagt gttcgcgcaa aatgatgaga tggctttggg
      361 tgcaattaaa gcaattgagg ctgcgaaccg ccaaggtatt atcgtggtcg gcttcgacgg
      421 tacagaggac gcgttgaagg cgattaaaga agggaagatg gcggcgacca ttgcacaaca
      481 gccggccttg atgggctcgc ttggggtcga gatggctgat aaatatctta agggcgaaaa
      541 aatccctaat tttatccccg ctgaattgaa attgatcact aaagaaaatg tgcaaggggg
      601 ttctgcctct ggagggacaa gtggtggatc gagtgcggcc ggtttggagg ttttattcca
      661 gggtccagcg gccgcaggca tggaggagcc gcagtcagat cctagcgtcg agccccctct
      721 gagtcaggaa acattttcag acctatggaa actacttcct gaaaacaacg ttctgtcccc
      781 cttgccgtcc caagcaatgg atgatttgat gctgtccccg gacgatattg aacaatggtt
      841 cactgaagac ccaggtccag atgaagctcc cagaatgcca gaggctgctc cccccgtggc
      901 ccctgcacca gcagctccta caccggcggc ccctgcacca gccccctcct ggcccctgtc
      961 atcttctgtc ccttcccaga aaacctacca gggcagctac ggtttccgtc tgggcttctt
     1021 gcattctggg acagccaagt ctgtgacttg cacgtactcc cctgccctca acaagatgtt
     1081 ttgccaactg gccaagacct gccctgtgca gctgtgggtt gattccacac ccccgcccgg
     1141 cacccgcgtc cgcgccatgg ccatctacaa gcagtcacag cacatgacgg aggttgtgag
     1201 gcgctgcccc caccatgagc gctgctcaga tagcgatggt ctggcccctc ctcagcatct
     1261 tatccgagtg gaaggaaatt tgcgtgtgga gtatttggat gacagaaaca cttttcgaca
     1321 tagtgtggtg gtgccctatg agccgcctga ggttggctct gactgtacca ccatccacta
     1381 caactacatg tgtaacagtt cctgcatggg cggcatgaac cggaggccca tcctcaccat
     1441 catcacactg gaagactcca gtggtaatct actgggacgg aacagctttg aggtgcgtgt
     1501 ttgtgcctgt cctgggagag accggcgcac agaggaagag aatctccgca agaaagggga
     1561 gcctcaccac gagctgcccc cagggagcac taagcgagca ctgcccaaca acaccagctc
     1621 ctctccccag ccaaagaaga aaccactgga tggagaatat ttcacccttc agatccgtgg
     1681 gcgtgagcgc ttcgagatgt tccgagagct gaatgaggcc ttggaactca aggatgccca
     1741 ggctgggaag gagccagggg ggagcagggc tcactccagc cacctgaagt ccaaaaaggg
     1801 tcagtctacc tcccgccata aaaaactcat gttcaagaca gaagggcctg actcagacgg
     1861 cggatccggt ggcttagaag tattattcca aggtccaggt tcgtcaggag ggacggcatc
     1921 tggagggaaa gaaggcaaaa ccatcggtct ggtcatcagt accttgaata accccttctt
     1981 tgtaaccttg aaaaatggcg ctgaggagaa agcgaaggaa ctgggataca agatcatcgt
     2041 agaggattcc cagaatgact cctcgaagga gttatccaac gttgaggatt taattcaaca
     2101 gaaggtcgac gtactgctga ttaaccctgt agattcggat gctgttgtaa ccgccattaa
     2161 ggaagcaaat agcaagaaca ttccagttat tactattgat cgctcggcca acgggtaatg
     2221 actcgagcac caccaccacc accaccacca ctaa
//
