LOCUS       sequence-338522-       13615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for human direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Garbutt TA, Zhou Y, Keepers B, Liu J, Qian L
  TITLE     An Optimized Protocol for Human Direct Cardiac Reprogramming.
  JOURNAL   STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100010. Epub
            2020 Jun 3.
  PUBMED    32728671
REFERENCE   2  (bases 1 to 13615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-06-19"; volume: "1(1). doi"; pages: " 
            10.1016/j.xpro.2019.100010. Epub 2020 Jun 3"
COMMENT     Sequence Label: pLL3.7-UBCp-hMGT-Ef1a-puro
FEATURES             Location/Qualifiers
     source          1..13615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             393..437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    735..852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        944..2155
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1992..2011
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3637..3693
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5041..5094
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        6722..6933
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6946..7214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7241..7840
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7241..7260)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7737..7757
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7847..8435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7900..7920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8318..8329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8509..8742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8814..8935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8851..8870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8905..8924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8975..9110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9130..9149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9131..9149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(9172..9530)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9212..9233)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9424..9443
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9557..9661
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9663..10020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9663..9854
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9686..9706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9871..10006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10055..10849
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10055..10849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10109..10128)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10719..10738
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11037..11056)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11081..11128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11457..12045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        12220..12523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12525..12723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12678..12698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             12741..12921
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12968..13093
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(13590..13615,1..208)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
       61 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
      121 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
      181 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
      241 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
      301 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
      361 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
      421 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
      481 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
      541 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
      601 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      661 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
      721 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      781 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      841 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc
      901 ccgctctagc cctagattta acccaacaga aggctcgacg cgtggcctcc gcgccgggtt
      961 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     1021 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     1081 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     1141 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     1201 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     1261 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     1321 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     1381 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     1441 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
     1501 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
     1561 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     1621 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     1681 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     1741 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     1801 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1861 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1921 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     1981 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     2041 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     2101 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgctag
     2161 actgccggat cgaattgtcg acgaattcgg atccgcggcc gcgtttaaac ttaattaacc
     2221 atcgataccg ccatggggag aaaaaagatt cagattacga ggattatgga tgaacgtaac
     2281 agacaggtga catttacaaa gaggaaattt gggttgatga agaaggctta tgagctgagc
     2341 gtgctgtgtg actgtgagat tgcgctgatc atcttcaaca gcaccaacaa gctgttccag
     2401 tatgccagca ccgacatgga caaagtgctt ctcaagtaca cggagtacaa cgagccgcat
     2461 gagagccgga caaactcaga catcgtggag gcattgaaca agaaagaaaa caaaggctgt
     2521 gaaagccccg atcccgactc ctcttatgca ctcaccccac gcactgaaga aaaatacaaa
     2581 aaaattaatg aagaatttga taatatgatc aagagtcata aaattcctgc tgttccacct
     2641 cccaacttcg agatgccagt ctccatccca gtgtccagcc acaacagttt ggtgtacagc
     2701 aaccctgtca gctcactggg aaaccccaac ctattgccac tggctcaccc ttctctgcag
     2761 aggaatagta tgtctcctgg tgtaacacat cgacctccaa gtgcaggtaa cacaggtggt
     2821 ctgatgggtg gagacctcac gtctggtgca ggcaccagtg cagggaacgg gtatggcaat
     2881 ccccgaaact caccaggtct gctggtctca cctggtaact tgaacaagaa tatgcaagca
     2941 aaatctcctc ccccaatgaa tttaggaatg aataaccgta aaccagatct ccgagttctt
     3001 attccaccag gcagcaagaa tacgatgcca tcagtgaatc aaaggataaa taactcccag
     3061 tcggctcagt cattggctac cccagtggtt tccgtagcaa ctcctacttt accaggacaa
     3121 ggaatgggag gatatccatc agccatttca acaacatatg gtaccgagta ctctctgagt
     3181 agtgcagacc tgtcatctct gtctgggttt aacaccgcca gcgctcttca ccttggttca
     3241 gtaactggct ggcaacagca acacctacat aacatgccac catctgccct cagtcagttg
     3301 ggagcttgca ctagcactca tttatctcag agttcaaatc tctccctgcc ttctactcaa
     3361 agcctcaaca tcaagtcaga acctgtttct cctcctagag accgtaccac caccccttcg
     3421 agatacccac aacacacgcg ccacgaggcg gggagatctc ctgttgacag cttgagcagc
     3481 tgtagcagtt cgtacgacgg gagcgaccga gaggatcacc ggaacgaatt ccactccccc
     3541 attggactca ccagaccttc gccggacgaa agggaaagtc cctcagtcaa gcgcatgcga
     3601 ctttctgaag gatgggcaac agctagcggc agcggcgcca caaacttctc tctgctaaag
     3661 caagcaggtg atgttgaaga aaaccccggg cctactagta tgtatcagag cttggccatg
     3721 gccgccaacc acgggccgcc ccccggtgcc tacgaggcgg gcggccccgg cgccttcatg
     3781 cacggcgcgg gcgccgcgtc ctcgccagtc tacgtgccca caccgcgggt gccctcctcc
     3841 gtgctgggcc tgtcctacct ccagggcgga ggcgcgggct ctgcgtccgg aggcgcctcg
     3901 ggcggcagct ccggtggggc cgcgtctggt gcggggcccg ggacccagca gggcagcccg
     3961 ggatggagcc aggcgggagc cgacggagcc gcttacaccc cgccgccggt gtcgccgcgc
     4021 ttctccttcc cggggaccac cgggtccctg gcggccgccg ccgccgctgc cgcggcccgg
     4081 gaagctgcgg cctacagcag tggcggcgga gcggcgggtg cgggcctggc gggccgcgag
     4141 cagtacgggc gcgccggctt cgcgggctcc tactccagcc cctacccggc ttacatggcc
     4201 gacgtgggcg cgtcctgggc cgcagccgcc gccgcctccg ccggcccctt cgacagcccg
     4261 gtcctgcaca gcctgcccgg ccgggccaac ccggccgccc gacaccccaa tctcgatatg
     4321 tttgacgact tctcagaagg cagagagtgt gtcaactgtg gggctatgtc caccccgctc
     4381 tggaggcgag atgggacggg tcactatctg tgcaacgcct gcggcctcta ccacaagatg
     4441 aacggcatca accggccgct catcaagcct cagcgccggc tgtccgcctc ccgccgagtg
     4501 ggcctctcct gtgccaactg ccagaccacc accaccacgc tgtggcgccg caatgcggag
     4561 ggcgagcctg tgtgcaatgc ctgcggcctc tacatgaagc tccacggggt ccccaggcct
     4621 cttgcaatgc ggaaagaggg gatccaaacc agaaaacgga agcccaagaa cctgaataaa
     4681 tctaagacac cagcagctcc ttcaggcagt gagagccttc ctcccgccag cggtgcttcc
     4741 agcaactcca gcaacgccac caccagcagc agcgaggaga tgcgtcccat caagacggag
     4801 cctggcctgt catctcacta cgggcacagc agctccgtgt cccagacgtt ctcagtcagt
     4861 gcgatgtctg gccatgggcc ctccatccac cctgtcctct cggccctgaa gctctcccca
     4921 caaggctatg cgtctcccgt cagccagtct ccacagacca gctccaagca ggactcttgg
     4981 aacagcctgg tcttggccga cagtcacggg gacataatca ctgcgaagct tggctccggc
     5041 gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgac
     5101 atggccgacg cagacgaggg ctttggcctg gcgcacacgc ctctggagcc tgacgcaaaa
     5161 gacctgccct gcgattcgaa acccgagagc gcgctcgggg cccccagcaa gtccccgtcg
     5221 tccccgcagg ccgccttcac ccagcagggc atggagggaa tcaaagtgtt tctccatgaa
     5281 agagaactgt ggctaaaatt ccacgaagtg ggcacggaaa tgatcataac caaggctgga
     5341 aggcggatgt ttcccagtta caaagtgaag gtgacgggcc ttaatcccaa aacgaagtac
     5401 attcttctca tggacattgt acctgccgac gatcacagat acaaattcgc agataataaa
     5461 tggtctgtga cgggcaaagc tgagcccgcc atgcctggcc gcctgtacgt gcacccagac
     5521 tcccccgcca ccggggcgca ttggatgagg cagctcgtct ccttccagaa actcaagctc
     5581 accaacaacc acctggaccc atttgggcat attattctaa attccatgca caaataccag
     5641 cctagattac acatcgtgaa agcggatgaa aataatggat ttggctcaaa aaatacagcg
     5701 ttctgcactc acgtctttcc tgagactgcg tttatagcag tgacttccta ccagaaccac
     5761 aagatcacgc aattaaagat tgagaataat ccctttgcca aaggatttcg gggcagtgat
     5821 gacatggagc tgcacagaat gtcaagaatg caaagtaaag aatatcccgt ggtccccagg
     5881 agcaccgtga ggcaaaaagt ggcctccaac cacagtcctt tcagcagcga gtctcgagct
     5941 ctctccacct catccaattt ggggtcccaa taccagtgtg agaatggtgt ttccggcccc
     6001 tcccaggacc tcctgcctcc acccaaccca tacccactgc cccaggagca tagccaaatt
     6061 taccattgta ccaagaggaa agaggaagaa tgttccacca cagaccatcc ctataagaag
     6121 ccctacatgg agacatcacc cagtgaagaa gattccttct accgctctag ctatccacag
     6181 cagcagggcc tgggtgcctc ctacaggaca gagtcggcac agcggcaagc ttgcatgtat
     6241 gccagctctg cgccccccag cgagcctgtg cccagcctag aggacatcag ctgcaacacg
     6301 tggccaagca tgccttccta cagcagctgc accgtcacca ccgtgcagcc catggacagg
     6361 ctaccctacc agcacttctc cgctcacttc acctcggggc ccctggtccc tcggctggct
     6421 ggcatggcca accatggctc cccacagctg ggagagggaa tgttccagca ccagacctcc
     6481 gtggcccacc agcctgtggt caggcagtgt gggcctcaga ctggcctgca gtcccctggc
     6541 acccttcagc cccctgagtt cctctactct catggcgtgc caaggactct atcccctcat
     6601 cagtaccact ctgtgcacgg agttggcatg gtgccagagt ggagcgacaa tagctaatta
     6661 attaaggccg gccctcgagg ttggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6721 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6781 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6841 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6901 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6961 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     7021 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     7081 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     7141 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     7201 cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac
     7261 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     7321 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     7381 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7441 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7501 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7561 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7621 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7681 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7741 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7801 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga
     7861 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7921 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7981 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8041 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8101 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8161 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8221 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8281 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8341 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8401 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     8461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8521 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8941 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9121 cccaattcgc cctatagtga gtcgtattag catgtgagca aaaggccagc ataaatcaaa
     9181 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9241 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9301 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9361 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9421 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     9481 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     9541 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9661 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     9721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     9781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10021 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
    10081 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
    10141 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
    10201 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
    10261 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
    10321 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
    10381 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
    10441 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
    10501 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
    10561 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
    10621 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
    10681 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
    10741 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
    10801 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
    10861 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
    10921 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10981 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
    11041 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    11101 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
    11161 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
    11221 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
    11281 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
    11341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    11401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    11461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    11521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    11581 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    11641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    12001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    12061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    12121 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
    12181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    12241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    12301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    12361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    12421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    12481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    12541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    12601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    12661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    12721 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    12781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    12841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    12901 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    12961 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    13021 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    13081 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    13141 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    13201 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    13261 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    13321 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    13381 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    13441 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    13501 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    13561 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgg
//
