LOCUS       sequence-338526-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018311
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..50)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(204..792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(284..303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      1255..1886
                     /label=oriV
                     /note="incP origin of replication"
     oriT            2039..2148
                     /note="incP origin of transfer"
     CDS             2181..2552
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             2824..3972
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4203..5402)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     primer_bind     6188..6206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6244..6266)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6366..6385
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6579..6601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6593..6610
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6594..6610
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6611..6667
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(6693..6716)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(6717..6737)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             8547..8569
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(9255..9271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9255..9271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9268..9290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9279..9295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9303..9309,9310..9327,9328..9333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9348..9369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
       61 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1141 aaccagccag ccggaagggc cgagcgcaga agtggtcagc ttggctgcca tttttggggt
     1201 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg
     1261 ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg
     1321 cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag
     1381 gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg
     1441 acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg
     1501 tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg
     1561 cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt
     1621 cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat
     1681 ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt
     1741 cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac
     1801 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
     1861 cgagggcaac cagcccggtg agcgtcggaa agggtcgacg gatcttttcc gctgcataac
     1921 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     1981 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     2041 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     2101 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg
     2161 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac
     2221 ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg
     2281 ccggcatgag cctgtaggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg
     2341 tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg
     2401 gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca
     2461 cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc aaggtcatga
     2521 tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa cggccggggg
     2581 gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga cttcgcggag
     2641 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     2701 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     2761 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     2821 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     2881 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     2941 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     3001 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     3061 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     3121 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     3181 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     3241 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     3301 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     3361 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     3421 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     3481 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     3541 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     3601 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
     3661 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
     3721 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
     3781 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
     3841 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
     3901 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
     3961 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcttta
     4021 ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca gtatcgctcg
     4081 ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg acaattctag
     4141 ggcgcgtata gcttgccgga agtcgccttg acccgcatgg cataggccta tcgtttccac
     4201 gatcagcgat cggctcgttg ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg
     4261 cagagcaagt agagggcagc gcctgcaatc catgcccacc cgttccacgt tgttatagaa
     4321 gccgcataga tcgccgtgaa gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc
     4381 gccagtgagc cttgcagctg cccctgacgt tcctcatcca cctgcctgga caacattgct
     4441 tgcagcgccg gcattccgat gccacccgaa gcaagcagga ccatgatcgg gaacgccatc
     4501 catccccgtg tcgcgaaggc aagcaggatg tagcctgtgc cgtcggcaat cattccgagc
     4561 atgagtgccc gcctttcgcc gagccgggcg gctacagggc cggtgatcat tgcctgggcg
     4621 agtgaatgca gaatgccaaa tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga
     4681 aagcgatcct cgccgaaaat gacccaaagc gcggccggca cctgtccgac aagttgcatg
     4741 atgaagaaga ccgccatcag ggcggcgacg acggtcatgc cccgggccca ccggaacgaa
     4801 gcgagcgggt tgagagcctc ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc
     4861 aaaaggaaac agcccgtcag gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga
     4921 gcgtgggggg agaaaccgcc catcagccca ccgagcacag gtcccgcgac catcccgaac
     4981 ccgaaacagg cgctcatgaa gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg
     5041 gcaatataag cgccggctac cgccccagtc gccccggtga tgccggccac gatccgcccg
     5101 atatagagaa cccaaaggaa aggcgctgtc gccatgatgg cgtagtcgac agtggcgccg
     5161 gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca
     5221 ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg
     5281 acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg
     5341 atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc
     5401 acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact
     5461 gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc
     5521 agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc
     5581 tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact
     5641 tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata
     5701 cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca
     5761 gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac
     5821 cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt
     5881 tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg
     5941 tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg
     6001 ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg
     6061 cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg
     6121 tcaggaacgt tgaaggaccg agaaagggtg ctaagaaacc attattatca tgacattaac
     6181 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6241 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6301 gagcatacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6361 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     6481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6601 gacggccagt gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
     6661 caagcttggc gtaatcatgg tcatagctgt cagtggtgat ggtgatggtg atgatgtccc
     6721 tgaaaataga ggttttcggc gggcagagcg aagacggtca tcaccccgcc cagctcggtg
     6781 tactgtgcca gctcggcgta gccgcccacg gcgcccaacc cgtcggtcga cttctcgagg
     6841 ccggcagcca ggccgatccc cgcccagccg ccgatacccg acaggatgcc gatgaactgc
     6901 tttcccttga actcccaggt tgtcacattg ccgatgatgc cggacggggt cttgaacttg
     6961 tagagctcct tggtgatatc gtcggcatcc acacacttga ggtagccctc gagggtgccg
     7021 tagcacgcga tgccgccggc ggtgaccagg gtgcccgacc agacgctgaa tttttcgggc
     7081 ttcgactgga cgatcttgcc cttcccggcg tcccaggtga tgaagttccc catgccgcca
     7141 tgcgaccccg gcgccgggta catcgaaagc gtcgcgccga cgtacggttg cccggccgtg
     7201 tattcgacgc ggaacggctc ataatccatg cacacgtggt tggtcggcac gtagaacagg
     7261 ccattcttgg ggttgaacga ggccggctgc tggtccttgg tgccgagggc ggccgggcag
     7321 atgcccttgc tgttcacgtc ctcgccgttc agccgggtcg agtacttcgc gaccacctgt
     7381 gggcggccgg tgaccatgtc cacgtgcgtg gcccagttga ccttcgggtc gaacttctcg
     7441 gcgaccagca gctcgccggt aacgcggtcg agggtgtagg cgaagccgtt acggtcgaaa
     7501 tggacggcga ccttccggcg cttgcccttg acgtcgatgt cggcaaggat gacctcattc
     7561 acaccgtcga aatcccattc gtcgtggggg gtcatctggt acacccaccg taccttgccc
     7621 gtatccacgt cgcgggccca cagcgtcatg gaccacttgt tatcacccgg gcgctggctc
     7681 gggttccacg tcgacgggtt gccagtcccg tagtacatca ggttctcctg cggatcgtaa
     7741 gagtaccagc cccaagtgct accgccgcca atcttccact ggtccccctg ccaggttttg
     7801 agcgacgagt ccttgccgac cggggccatc ttgccgttcg tccaggtcat cgacttctcc
     7861 gggtccatga gcatctccgc gtcaggcccg gtcgagtagc ctttccagac catcttgcca
     7921 gtgttaaggt cgtacgcggt gacccgcccg cgcacgccga actcgccgcc cgagatgccg
     7981 gtgatcacct tgtccttgaa cacatgcggg gcctgggtat tcgtctcgcc gattttgggg
     8041 tcgccgttct tgacgtccca gagcttcttg ccggtcttgg cgtccagggc cacgaggacg
     8101 gtgtcggcct gttggagcag gatcttgccc tcggcgtaag cgagcccgcg gttcacggtg
     8161 tcgcagcaca tgactgggat aaccgacggg tcctgcttgg gctcgtactt ccagacgatc
     8221 ttctgggtct cgaggtcgat ggcgaacact ttgttcggga acgggctgtg gacgaagagc
     8281 atgccgccga tcaccagcgg gcccccctcg tggccccgga ggacccccgt gctgaaggtc
     8341 caggccacct tcaggtcctt cacgtttttc ttgttgatct ggttaagggt cgagtaccgc
     8401 tggttttcga agttgcccgc ctgcatggcc cagttcttag gatccttgat tagcttggcc
     8461 agatccgcgt tggccatagc ggcgccgggg actgccagcg cggcgagcca gaggggcagc
     8521 tgcttcatct tgaccgacat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
     8581 aggattcctc cgacaagtct tatccgccgc tggatagcag cccgctaccc accgttgatt
     8641 gacctgctgt ttgcgacagg gatgatattc gctccggcag ctcgcgcctg ccgcacagaa
     8701 attaggatta ttccatagtt cccggcgact tacccggctg ggtccgcatc ccttagcggc
     8761 tccccgaaac cgaagcttga acgaactcta agaaaatttc gggcgccgtc aactcgcttt
     8821 cgcgcgtcga atgtcgcact cacagggccc tttatcagga aacttgcctg ctaaattcag
     8881 cattttgcgt tgcgaaattt cctgtgtgag atccgcggta tctctcagac gtttttctga
     8941 ggttccaggc tcgctcggaa gtcatccgaa gtgccgcatc gtgaatctaa ggatattttt
     9001 agatttgcgc ggtcttatga aagacttatt agcgtccctt cgcctatatt ttctaggctt
     9061 tgattggacg cgatgtcttt agctggtcgg acatttctta tcaggacgat aaggcaattc
     9121 taaaagtgcc aaggcgtagt gtcgcaacgt attggcatcg tcttgaccgg ggccgggacc
     9181 catcgcaccg ttgtcgtcaa cgggccctcg cgaagcggcc cgaccaagcg ggaagctagc
     9241 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9421 ctgcattaat gaatcggcca acgcgcgggg aga
//
