LOCUS       sequence-338527-       12922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for human direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Garbutt TA, Zhou Y, Keepers B, Liu J, Qian L
  TITLE     An Optimized Protocol for Human Direct Cardiac Reprogramming.
  JOURNAL   STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100010. Epub
            2020 Jun 3.
  PUBMED    32728671
REFERENCE   2  (bases 1 to 12922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-06-19"; volume: "1(1). doi"; pages: " 
            10.1016/j.xpro.2019.100010. Epub 2020 Jun 3"
COMMENT     Sequence Label: pLenti-Ef1a-hMGT-PGK-puro
FEATURES             Location/Qualifiers
     source          1..12922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4179..4390
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4391..5329
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5286..5306
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6767..6823
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8171..8224
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9800..10388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9853..9873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10271..10282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10402..10901
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     10834..10853
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10922..11521
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10922..10941)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11418..11438
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11659..11892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11964..12085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12001..12020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12055..12074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12125..12260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12280..12299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12281..12299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12309..12326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12309..12325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12318..12340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12467..12895
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12554..12573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12764..12785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tagtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 atgaggccct ttcgtcttca ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac
     4201 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     4261 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4321 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4381 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     4441 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     4501 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     4561 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     4621 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     4681 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     4741 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     4801 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     4861 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     4921 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     4981 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     5041 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     5101 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     5161 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     5221 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     5281 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagt
     5341 ttaattaacc atcgataccg ccatggggag aaaaaagatt cagattacga ggattatgga
     5401 tgaacgtaac agacaggtga catttacaaa gaggaaattt gggttgatga agaaggctta
     5461 tgagctgagc gtgctgtgtg actgtgagat tgcgctgatc atcttcaaca gcaccaacaa
     5521 gctgttccag tatgccagca ccgacatgga caaagtgctt ctcaagtaca cggagtacaa
     5581 cgagccgcat gagagccgga caaactcaga catcgtggag gcattgaaca agaaagaaaa
     5641 caaaggctgt gaaagccccg atcccgactc ctcttatgca ctcaccccac gcactgaaga
     5701 aaaatacaaa aaaattaatg aagaatttga taatatgatc aagagtcata aaattcctgc
     5761 tgttccacct cccaacttcg agatgccagt ctccatccca gtgtccagcc acaacagttt
     5821 ggtgtacagc aaccctgtca gctcactggg aaaccccaac ctattgccac tggctcaccc
     5881 ttctctgcag aggaatagta tgtctcctgg tgtaacacat cgacctccaa gtgcaggtaa
     5941 cacaggtggt ctgatgggtg gagacctcac gtctggtgca ggcaccagtg cagggaacgg
     6001 gtatggcaat ccccgaaact caccaggtct gctggtctca cctggtaact tgaacaagaa
     6061 tatgcaagca aaatctcctc ccccaatgaa tttaggaatg aataaccgta aaccagatct
     6121 ccgagttctt attccaccag gcagcaagaa tacgatgcca tcagtgaatc aaaggataaa
     6181 taactcccag tcggctcagt cattggctac cccagtggtt tccgtagcaa ctcctacttt
     6241 accaggacaa ggaatgggag gatatccatc agccatttca acaacatatg gtaccgagta
     6301 ctctctgagt agtgcagacc tgtcatctct gtctgggttt aacaccgcca gcgctcttca
     6361 ccttggttca gtaactggct ggcaacagca acacctacat aacatgccac catctgccct
     6421 cagtcagttg ggagcttgca ctagcactca tttatctcag agttcaaatc tctccctgcc
     6481 ttctactcaa agcctcaaca tcaagtcaga acctgtttct cctcctagag accgtaccac
     6541 caccccttcg agatacccac aacacacgcg ccacgaggcg gggagatctc ctgttgacag
     6601 cttgagcagc tgtagcagtt cgtacgacgg gagcgaccga gaggatcacc ggaacgaatt
     6661 ccactccccc attggactca ccagaccttc gccggacgaa agggaaagtc cctcagtcaa
     6721 gcgcatgcga ctttctgaag gatgggcaac agctagcggc agcggcgcca caaacttctc
     6781 tctgctaaag caagcaggtg atgttgaaga aaaccccggg cctactagta tgtatcagag
     6841 cttggccatg gccgccaacc acgggccgcc ccccggtgcc tacgaggcgg gcggccccgg
     6901 cgccttcatg cacggcgcgg gcgccgcgtc ctcgccagtc tacgtgccca caccgcgggt
     6961 gccctcctcc gtgctgggcc tgtcctacct ccagggcgga ggcgcgggct ctgcgtccgg
     7021 aggcgcctcg ggcggcagct ccggtggggc cgcgtctggt gcggggcccg ggacccagca
     7081 gggcagcccg ggatggagcc aggcgggagc cgacggagcc gcttacaccc cgccgccggt
     7141 gtcgccgcgc ttctccttcc cggggaccac cgggtccctg gcggccgccg ccgccgctgc
     7201 cgcggcccgg gaagctgcgg cctacagcag tggcggcgga gcggcgggtg cgggcctggc
     7261 gggccgcgag cagtacgggc gcgccggctt cgcgggctcc tactccagcc cctacccggc
     7321 ttacatggcc gacgtgggcg cgtcctgggc cgcagccgcc gccgcctccg ccggcccctt
     7381 cgacagcccg gtcctgcaca gcctgcccgg ccgggccaac ccggccgccc gacaccccaa
     7441 tctcgatatg tttgacgact tctcagaagg cagagagtgt gtcaactgtg gggctatgtc
     7501 caccccgctc tggaggcgag atgggacggg tcactatctg tgcaacgcct gcggcctcta
     7561 ccacaagatg aacggcatca accggccgct catcaagcct cagcgccggc tgtccgcctc
     7621 ccgccgagtg ggcctctcct gtgccaactg ccagaccacc accaccacgc tgtggcgccg
     7681 caatgcggag ggcgagcctg tgtgcaatgc ctgcggcctc tacatgaagc tccacggggt
     7741 ccccaggcct cttgcaatgc ggaaagaggg gatccaaacc agaaaacgga agcccaagaa
     7801 cctgaataaa tctaagacac cagcagctcc ttcaggcagt gagagccttc ctcccgccag
     7861 cggtgcttcc agcaactcca gcaacgccac caccagcagc agcgaggaga tgcgtcccat
     7921 caagacggag cctggcctgt catctcacta cgggcacagc agctccgtgt cccagacgtt
     7981 ctcagtcagt gcgatgtctg gccatgggcc ctccatccac cctgtcctct cggccctgaa
     8041 gctctcccca caaggctatg cgtctcccgt cagccagtct ccacagacca gctccaagca
     8101 ggactcttgg aacagcctgg tcttggccga cagtcacggg gacataatca ctgcgaagct
     8161 tggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
     8221 cccactcgac atggccgacg cagacgaggg ctttggcctg gcgcacacgc ctctggagcc
     8281 tgacgcaaaa gacctgccct gcgattcgaa acccgagagc gcgctcgggg cccccagcaa
     8341 gtccccgtcg tccccgcagg ccgccttcac ccagcagggc atggagggaa tcaaagtgtt
     8401 tctccatgaa agagaactgt ggctaaaatt ccacgaagtg ggcacggaaa tgatcataac
     8461 caaggctgga aggcggatgt ttcccagtta caaagtgaag gtgacgggcc ttaatcccaa
     8521 aacgaagtac attcttctca tggacattgt acctgccgac gatcacagat acaaattcgc
     8581 agataataaa tggtctgtga cgggcaaagc tgagcccgcc atgcctggcc gcctgtacgt
     8641 gcacccagac tcccccgcca ccggggcgca ttggatgagg cagctcgtct ccttccagaa
     8701 actcaagctc accaacaacc acctggaccc atttgggcat attattctaa attccatgca
     8761 caaataccag cctagattac acatcgtgaa agcggatgaa aataatggat ttggctcaaa
     8821 aaatacagcg ttctgcactc acgtctttcc tgagactgcg tttatagcag tgacttccta
     8881 ccagaaccac aagatcacgc aattaaagat tgagaataat ccctttgcca aaggatttcg
     8941 gggcagtgat gacatggagc tgcacagaat gtcaagaatg caaagtaaag aatatcccgt
     9001 ggtccccagg agcaccgtga ggcaaaaagt ggcctccaac cacagtcctt tcagcagcga
     9061 gtctcgagct ctctccacct catccaattt ggggtcccaa taccagtgtg agaatggtgt
     9121 ttccggcccc tcccaggacc tcctgcctcc acccaaccca tacccactgc cccaggagca
     9181 tagccaaatt taccattgta ccaagaggaa agaggaagaa tgttccacca cagaccatcc
     9241 ctataagaag ccctacatgg agacatcacc cagtgaagaa gattccttct accgctctag
     9301 ctatccacag cagcagggcc tgggtgcctc ctacaggaca gagtcggcac agcggcaagc
     9361 ttgcatgtat gccagctctg cgccccccag cgagcctgtg cccagcctag aggacatcag
     9421 ctgcaacacg tggccaagca tgccttccta cagcagctgc accgtcacca ccgtgcagcc
     9481 catggacagg ctaccctacc agcacttctc cgctcacttc acctcggggc ccctggtccc
     9541 tcggctggct ggcatggcca accatggctc cccacagctg ggagagggaa tgttccagca
     9601 ccagacctcc gtggcccacc agcctgtggt caggcagtgt gggcctcaga ctggcctgca
     9661 gtcccctggc acccttcagc cccctgagtt cctctactct catggcgtgc caaggactct
     9721 atcccctcat cagtaccact ctgtgcacgg agttggcatg gtgccagagt ggagcgacaa
     9781 tagctaatta attaacgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9841 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9901 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9961 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10021 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10081 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
    10201 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10261 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10321 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10381 ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
    10441 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
    10501 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
    10561 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
    10621 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
    10681 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
    10741 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
    10801 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
    10861 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
    10921 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    10981 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    11041 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    11101 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
    11161 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    11221 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    11281 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    11341 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    11401 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    11461 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
    11521 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg
    11581 agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    11641 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt
    11701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    11761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    11821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    11881 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    11941 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    12001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12061 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    12121 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    12181 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12241 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    12301 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    12361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    12421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    12481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    12541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    12601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    12661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    12721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    12781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    12841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    12901 aaaatattaa cgcttacaat tt
//
