LOCUS       sequence-338529-        7043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for murine direct cardiac 
            reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Wang L, Huang P, Near D, Ravi K, Xu Y, Liu J, Qian L
  TITLE     Isoform Specific Effects of Mef2C during Direct Cardiac 
            Reprogramming.
  JOURNAL   Cells. 2020 Jan 22;9(2). pii: cells9020268. doi: 
            10.3390/cells9020268.
  PUBMED    31979018
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-01-22"; volume: "9(2). pii: cells9020268. doi"; pages: "
            10.3390/cells9020268"
COMMENT     Sequence Label: pmx-puro-Mef2c-i
FEATURES             Location/Qualifiers
     source          1..7043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(3378..3398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3383..3712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3563..3698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3722..4321
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3722..3741)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4218..4238
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4548..5017
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5147..5164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5318..5906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5398..5417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6070..6861,6862..6930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6693..6712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6931..7035)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccatggg gagaaaaaag attcagatta cgaggataat ggatgagcgt aacagacagg
     1981 tgacttttac gaagaggaaa tttggattga tgaagaaggc ttatgagctg agcgtgctgt
     2041 gtgactgtga gattgcactg atcatcttca acagcaccaa caagctgttc cagtacgcca
     2101 gcactgacat ggataaggtg ttgctcaagt acaccgagta caacgagccg cacgagagcc
     2161 ggacaaactc agacattgtg gagacattga gaaagaaggg cctcaatggc tgtgacagcc
     2221 cagatcccga tgcagacgat tcagtaggtc acagccctga gtctgaggac aagtacagga
     2281 aaattaacga agatattgat ctaatgatca gcaggcaaag attgtgtgct gttccacctc
     2341 ccagctttga gatgccagtt accatcccag tgtccagcca taacagtttg gtgtacagca
     2401 atcctgtcag cacactggga aaccccaatc ttctgccact ggcccacccg tctctgcaga
     2461 ggaatagtat gtctcctggt gtaacacata gacctccaag tgcaggtaac acaggcggtc
     2521 tgatgggcgg agatctgaca tccggtgcag gcaccagcgc agggaatgga tacggcaacc
     2581 cccggaactc accaggcctg ctggtctcac ctggtaacct gaacaagaat atacaagcca
     2641 aatctcctcc ccctatgaat ctaggaatga ataatcgtaa gccagatctc cgcgttctta
     2701 tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat aactcccagt
     2761 cggctcagtc attggctacc ccggtggttt ccgtagcaac tcctacttta ccaggacaag
     2821 gaatgggagg atatccatca gccatttcaa caacatatgg tactgagtac tctctgagta
     2881 gcgcagatct gtcatctctg tctggcttca acactgccag tgcgctccac ctcggctctg
     2941 taactggctg gcagcagcag cacctacata acatgccgcc atctgccctc agtcagttgg
     3001 gagcttgcac tagcactcat ttatctcaga gttcaaatct ctccctgcct tctactcaaa
     3061 gcctcagcat caagtcagaa cctgtttctc ctcctagaga ccgtaccacc accccttcga
     3121 gatacccaca acacaccacg cgccacgagg cggggaggtc tcctgttgac agcttgagca
     3181 gctgtagcag ttcctacgat gggagcgacc gagaggatca ccggaacgaa ttccactccc
     3241 ccattggact caccagacct tcgccggacg aaagggaaag tccttcagtc aagcgcatgc
     3301 gactctctga aggatgggca acatgagcgg ccgccagcac agtggtcgag cggccgccag
     3361 cacagtggtc gacctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
     3721 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     3781 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     3841 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     3901 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     3961 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4021 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4081 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4141 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4201 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4261 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4321 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgtc
     4381 gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     4441 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     4501 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     4561 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     4621 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     4681 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     4741 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     4801 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     4861 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     4921 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     4981 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     5041 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     5101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6001 tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg
     6061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7021 aataggggtt ccgcgcacat ttc
//
