LOCUS       sequence-338536-        5114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Zhou Y, Liu Z, Welch JD, Gao X, Wang L, Garbutt T, Keepers B, Ma H, 
            Prins JF, Shen W, Liu J, Qian L
  TITLE     Single-Cell Transcriptomic Analyses of Cell Fate Transitions during 
            Human Cardiac Reprogramming.
  JOURNAL   Cell Stem Cell. 2019 Jul 3;25(1):149-164.e9. doi: 
            10.1016/j.stem.2019.05.020. Epub 2019 Jun 20.
  PUBMED    31230860
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.05.020"; journalName: "Cell Stem Cell"; date: 
            "2019-07-3- 3"; volume: "25"; issue: "1"; pages: "149-164.e9"
COMMENT     Sequence Label: pBabe-miR-133
FEATURES             Location/Qualifiers
     source          1..5114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..595
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    658..1015
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1080..1496
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1426..1448
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1464..1480
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1506..1880
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1818..1834
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             2599..3068
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3198..3215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3369..3957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3449..3468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4121..4912,4913..4981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4744..4763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4982..5086)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
       61 catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga acagatggaa
      121 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      181 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
      241 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
      301 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
      361 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
      421 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgattgac tgagtcgccc
      481 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
      541 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg
      601 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag
      661 ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg
      721 cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga
      781 cgagttcgga acacccggcc gcaaccctgg gagacgtccc agggacttcg ggggccgttt
      841 ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg actctttggt gcacccccct
      901 tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
      961 ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca
     1021 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg
     1081 gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga
     1141 tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag
     1201 aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca
     1261 cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct
     1321 acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac
     1381 accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc
     1441 gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccccca
     1501 tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg
     1561 acatgacaag agttactaac agcccctctc tccaagctca cttacaggct ctctacttag
     1621 tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg
     1681 tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga
     1741 acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca
     1801 aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg
     1861 gtggaccatc ctctagactg ccggatccca gtgtggtggt acgggaattc tccccttccc
     1921 agtaatgtcc tgggggtgcc attttggggc acatagagtg tctgaatgta catgtgaccc
     1981 ctcacacaca ccctgaagac ctccaaagcc cttgggtttg catgggttct cagagcaggg
     2041 agagcctggg acaggacagc agcctgacag aaccatcttc ttcctggagt ctctcctccc
     2101 agtggatcag aagccaaatg ctttgctgaa gctggtaaaa tggaaccaaa tcagctgttg
     2161 gatggatttg gtccccttca accagctgta gctgcgcatt gatcacgccg catggcccag
     2221 ccagaggaca cagccacagc aaggactctt caaaggctgc tgcagggagg cctccgcaat
     2281 ggtcagatcc aagaaagcca ggtcacgtgc gccaccagtg gccctgggtt gggatccagg
     2341 ctccccttgt ctcctcaaca ggcaagtaga ggaggaagct gaaacctccc taagctgtga
     2401 ctgacccagt gcggccgcca gcacagtggt cgacgataaa ataaaagatt ttatttagtc
     2461 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     2521 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     2581 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     2641 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     2701 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     2761 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     2821 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     2881 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     2941 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     3001 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     3061 gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt
     3121 aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3181 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3541 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3721 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3781 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca
     4081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5101 aagtgccacc tgca
//
