LOCUS       sequence-338543-        3105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of inactive PARP1 mutant (E988K attenuates 
            catalytic and PAR-branching activity of PARP1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-7"; volume: "118(36). pii: 
            2107277118. doi"; pages: " 10.1073/pnas.2107277118"
COMMENT     Sequence Label: pET28_6xHis-PARP1-E988K
FEATURES             Location/Qualifiers
     source          1..3105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggcggagt cttctgataa gctgtatcga gtcgagtacg ccaagagcgg gcgcgcctct
      121 tgcaagaaat gcagcgagag catccccaag gactcgctcc ggatggccat catggtgcag
      181 tcgcccatgt ttgatggaaa agtcccacac tggtaccact tctcctgctt ctggaaggtg
      241 ggccactcca tccggcaccc tgacgttgag gtggatgggt tctctgagct tcggtgggat
      301 gaccagcaga aagtcaagaa gacagcggaa gctggaggag tgacaggcaa aggccaggat
      361 ggaattggta gcaaggcaga gaagactctg ggtgactttg cagcagagta tgccaagtcc
      421 aacagaagta cgtgcaaggg gtgtatggag aagatagaaa agggccaggt gcgcctgtcc
      481 aagaagatgg tggacccgga gaagccacag ctaggcatga ttgaccgctg gtaccatcca
      541 ggctgctttg tcaagaacag ggaggagctg ggtttccggc ccgagtacag tgcgagtcag
      601 ctcaagggct tcagcctcct tgctacagag gataaggaag ccctgaagaa gcagctccca
      661 ggagtcaaga gtgaaggaaa gagaaaaggc gatgaggtgg atggagtgga tgaagtggcg
      721 aagaagaaat ctaaaaaaga aaaagacaag gatagtaagc ttgaaaaagc cctaaaggct
      781 cagaacgacc tgatctggaa catcaaggac gagctaaaga aagtgtgttc aactaatgac
      841 ctgaaggagc tactcatctt caacaagcag caagtgcctt ctggggagtc ggcgatcttg
      901 gaccgagtag ctgatggcat ggtgttcggt gccctccttc cctgcgagga atgctcgggt
      961 cagctggtct tcaagagcga tgcctattac tgcactgggg acgtcactgc ctggaccaag
     1021 tgtatggtca agacacagac acccaaccgg aaggagtggg taaccccaaa ggaattccga
     1081 gaaatctctt acctcaagaa attgaaggtt aaaaagcagg accgtatatt ccccccagaa
     1141 accagcgcct ccgtggcggc cacgcctccg ccctccacag cctcggctcc tgctgctgtg
     1201 aactcctctg cttcagcaga taagccatta tccaacatga agatcctgac tctcgggaag
     1261 ctgtcccgga acaaggatga agtgaaggcc atgattgaga aactcggggg gaagttgacg
     1321 gggacggcca acaaggcttc cctgtgcatc agcaccaaaa aggaggtgga aaagatgaat
     1381 aagaagatgg aggaagtaaa ggaagccaac atccgagttg tgtctgagga cttcctccag
     1441 gacgtctccg cctccaccaa gagccttcag gagttgttct tagcgcacat cttgtcccct
     1501 tggggggcag aggtgaaggc agagcctgtt gaagttgtgg ccccaagagg gaagtcaggg
     1561 gctgcgctct ccaaaaaaag caagggccag gtcaaggagg aaggtatcaa caaatctgaa
     1621 aagagaatga aattaactct taaaggagga gcagctgtgg atcctgattc tggactggaa
     1681 cactctgcgc atgtcctgga gaaaggtggg aaggtcttca gtgccaccct tggcctggtg
     1741 gacatcgtta aaggaaccaa ctcctactac aagctccagc ttctggagga cgacaaggaa
     1801 aacaggtatt ggatattcag gtcctggggc cgtgtgggta cggtgatcgg tagcaacaaa
     1861 ctggaacaga tgccgtccaa ggaggatgcc attgagcact tcatgaaatt atatgaagaa
     1921 aaaaccggga acgcttggca ctccaaaaat ttcacgaagt atcccaaaaa gttctacccc
     1981 ctggagattg actatggcca ggatgaagag gcagtgaaga agctgacagt aaatcctggc
     2041 accaagtcca agctccccaa gccagttcag gacctcatca agatgatctt tgatgtggaa
     2101 agtatgaaga aagccatggt ggagtatgag atcgaccttc agaagatgcc cttggggaag
     2161 ctgagcaaaa ggcagatcca ggccgcatac tccatcctca gtgaggtcca gcaggcggtg
     2221 tctcagggca gcagcgactc tcagatcctg gatctctcaa atcgctttta caccctgatc
     2281 ccccacgact ttgggatgaa gaagcctccg ctcctgaaca atgcagacag tgtgcaggcc
     2341 aaggcggaaa tgcttgacaa cctgctggac atcgaggtgg cctacagtct gctcagggga
     2401 gggtctgatg atagcagcaa ggatcccatc gatgtcaact atgagaagct caaaactgac
     2461 attaaggtgg ttgacagaga ttctgaagaa gccgagatca tcaggaagta tgttaagaac
     2521 actcatgcaa ccacacacaa tgcgtatgac ttggaagtca tcgatatctt taagatagag
     2581 cgtgaaggcg aatgccagcg ttacaagccc tttaagcagc ttcataaccg aagattgctg
     2641 tggcacgggt ccaggaccac caactttgct gggatcctgt cccagggtct tcggatagcc
     2701 ccgcctgaag cgcccgtgac aggctacatg tttggtaaag ggatctattt cgctgacatg
     2761 gtctccaaga gtgccaacta ctgccatacg tctcagggag acccaatagg cttaatcctg
     2821 ttgggagaag ttgcccttgg aaacatgtat gaactgaagc acgcttcaca tatcagcaag
     2881 ttacccaagg gcaagcacag tgtcaaaggt ttgggcaaaa ctacccctga tccttcagct
     2941 aacattagtc tggatggtgt agacgttcct cttgggaccg ggatttcatc tggtgtgaat
     3001 gacacctctc tactatataa caagtacatt gtctatgata ttgctcaggt aaatctgaag
     3061 tatctgctga aactgaaatt caattttaag acctccctgt ggtaa
//
