LOCUS       sequence-338562-        5252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the C-terminal (189-400) domain of SERBP1 in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Baudin A, Xu X, Libich DS
  TITLE     The (1)H, (15)N and (13)C resonance assignments of the C-terminal 
            domain of Serpine mRNA binding protein 1 (SERBP1).
  JOURNAL   Biomol NMR Assign. 2021 Oct;15(2):461-466. doi: 
            10.1007/s12104-021-10046-3. Epub 2021 Aug 26.
  PUBMED    34436734
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12104-021-10046-3"; journalName: "Biomol NMR Assign"; 
            date: "2021-10"; volume: "15"; issue: "2"; pages: "461-466"
COMMENT     Sequence Label: pAGHa-SERBP1(189-400)
FEATURES             Location/Qualifiers
     source          1..5252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..98
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        79..97
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             129..151
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             166..189
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(909..927)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      923..970
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1145..1150,1151..1168,1169..1174))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1201..1219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1287..1391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1392..1460,1461..2252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1610..1629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2423..3011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2912..2931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3165..3182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3197..3336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3262..3281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3422..3444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3438..3629)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcaaccgcac ctgtagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
       61 atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
      121 gaaataattt tgtttaactt taagaaggag atataccata tggaccacca tcaccaccac
      181 catcaccatg ctagcgagaa tctttatttt cagggcgccg gtaagcgtga gttcgaccgt
      241 cacagcggca gcgatcgtag cagcttcagc cactacagcg gtctgaaaca cgaagacaag
      301 cgtggtggca gcggtagcca caactggggc accgtgaagg atgagctgac cgaaagcccg
      361 aaatacatcc agaagcagat cagctacaac tacagcgacc tggatcagag caacgtgacc
      421 gaggaaaccc cggagggcga ggaacaccac ccggttgctg acaccgaaaa caaggagaac
      481 gaagtggagg aagttaaaga ggaaggcccg aaggagatga ccctggacga atggaaagct
      541 atccagaaca aagatcgtgc gaaggttgag ttcaacatcc gtaagccgaa cgaaggcgct
      601 gacggccagt ggaaaaaggg tttcgtgctg cacaaaagca agagcgagga agctcacgcg
      661 gaggacagcg ttatggatca ccacttccgt aaaccggcga acgacatcac cagccagctg
      721 gaaatcaact tcggtgatct gggtcgtccg ggtcgtggtg gccgtggtgg ccgtggtggc
      781 cgtggtcgtg gtggccgtcc gaaccgtggc agccgtaccg acaagagcag cgctagcgct
      841 ccggatgttg atgattaagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      901 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      961 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
     1021 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     1081 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
     1141 ccgcattaaa gcttatcgga tgataagctg tcaaacatga gaattcttga agacgaaagg
     1201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1621 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1921 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3181 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3241 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     3301 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     3361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     3481 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     3541 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     3601 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     3661 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     3721 gaacatgccc ggttactgga acgtcgtgag ggtaaacaac tggcggtatg gatgcggcgg
     3781 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     3841 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     3901 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     3961 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     4021 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     4081 atcatgcaca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     4141 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     4201 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     4261 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     4321 caaggtatag ggcggcacct acaatccatg acaaggtata gggcggcacc tacaatccat
     4381 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
     4441 atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     4501 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     4561 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     4621 cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     4681 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     4741 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     4801 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     4861 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     4921 tgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     4981 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggagcccaa cagtcccccc
     5041 atgcaaggag atggagccca acagtcccca tgcaaggaga tggagcccaa cagtcccacg
     5101 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     5161 gcccgatctt ccccatcggt gatgtcggcg atataggcgc aagcaaccgc acctgtagcg
     5221 ccggtgatgc cgggtcggcg atataggcgc aa
//
