LOCUS       sequence-338591-       12749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of BCL6-GFP G55A mutant fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Slabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, 
            Donovan KA, Sperling AS, Hunkeler M, Tsai JM, Sharma R, Guirguis A, 
            Zou C, Chudasama P, Gasser JA, Miller PG, Scholl C, Frohling S, 
            Nowak RP, Fischer ES, Ebert BL
  TITLE     Small-molecule-induced polymerization triggers degradation of BCL6.
  JOURNAL   Nature. 2020 Dec;588(7836):164-168. doi: 10.1038/s41586-020-2925-1. 
            Epub 2020 Nov 18.
  PUBMED    33208943
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2925-1"; journalName: "Nature"; date: "2020-12";
            volume: "588"; issue: "7836"; pages: "164-168"
COMMENT     Sequence Label: BCL6-GFP G55A in Artichoke
FEATURES             Location/Qualifiers
     source          1..12749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1918..2324
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2362..2371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2383..2407
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4529..4553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4618..4620,4621..5334)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4618..4620,4621..5331)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4660..4681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4921..4940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5268..5289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5358..5932,5933..5935,5936..5944)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5525..5542)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5752..5771
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5954..5963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5960..6670
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6105..6123)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6373..6392
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6726..6843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6977..8155
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7208..8146
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8103..8123
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8168..8767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8168..8187)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8664..8684
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8783..9371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8836..8856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9254..9265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9443..9676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9754..9875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9791..9810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9845..9864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9915..10050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10070..10089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10071..10089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10099..10116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10099..10115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10108..10130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10257..10712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10344..10363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10554..10575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10738..10842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10843..10911,10912..11703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11061..11080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11874..12462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12363..12382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12616..12633
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc tattccagca catatgagct agctgcagta
     1921 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     1981 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     2041 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     2101 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     2161 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     2221 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     2281 ccctcactcg gcgcgccagt cctccgacag actgagtcgg ccggtcgaat caagcttatc
     2341 gataccgtcg actccggaat agccaccatg gagacggatc aaacaagttt gtacaaaaaa
     2401 gttggcacca tggcctcgcc ggctgacagc tgtatccagt tcacccgcca tgccagtgat
     2461 gttcttctca accttaatcg tctccggagt cgagacatct tgactgatgt tgtcattgtt
     2521 gtgagccgtg agcagtttag agcccataaa acggtcctca tggcctgcag tgccctgttc
     2581 tatagcatct ttacagacca gttgaaatgc aaccttagtg tgatcaatct agatcctgag
     2641 atcaaccctg agggattctg catcctcctg gacttcatgt acacatctcg gctcaatttg
     2701 cgggagggca acatcatggc tgtgatggcc acggctatgt acctgcagat ggagcatgtt
     2761 gtggacactt gccggaagtt tattaaggcc agtgaagcag agatggtttc tgccatcaag
     2821 cctcctcgtg aagagttcct caacagccgg atgctgatgc cccaagacat catggcctat
     2881 cggggttgtg aggtggtgga gaacaacctg ccactgagga gcgcccctgg gtgtgagagc
     2941 agagcctttg cccccagcct gtacagtggc ctgtccacac cgccagcctc ttattccatg
     3001 tacagccacc tccctgtcag cagcctcctc ttctccgatg aggagtttcg ggatgtccgg
     3061 atgcctgtgg ccaacccctt ccccaaggag cgggcactcc catgtgatag tgccaggcca
     3121 gtccctggtg agtacagccg gccgactttg gaggtgtccc ccaatgtgtg ccacagcaat
     3181 atctattcac ccaaggaaac aatcccagaa gaggcacgaa gtgatatgca ctacagtgtg
     3241 gctgagggcc tcaaacctgc tgccccctca gcccgaaatg ccccctactt cccttgtgac
     3301 aaggccagca aagaagaaga gagaccctcc tcggaagatg agattgccct gcatttcgag
     3361 ccccccaatg cacccctgaa ccggaagggt ctggttagtc cacagagccc ccagaaatct
     3421 gactgccagc ccaactcgcc cacagagtcc tgcagcagta agaatgcctg catcctccag
     3481 gcttctggct cccctccagc caagagcccc actgacccca aagcctgcaa ctggaagaaa
     3541 tacaagttca tcgtgctcaa cagcctcaat cagaatgcca aaccagaggg gcctgagcag
     3601 gctgagctgg gccgcctttc cccacgagcc tacacggccc cacctgcctg ccagccaccc
     3661 atggagcctg agaaccttga cctccagtcc ccaaccaagc tgagtgccag cggggaggac
     3721 tccaccatcc cacaagccag ccggctcaat aacatcgtta acaggtccat gacgggctct
     3781 ccccgcagca gcagcgagag ccactcacca ctctacatgc accccccgaa gtgcacgtcc
     3841 tgcggctctc agtccccaca gcatgcagag atgtgcctcc acaccgctgg ccccacgttc
     3901 cctgaggaga tgggagagac ccagtctgag tactcagatt ctagctgtga gaacggggcc
     3961 ttcttctgca atgagtgtga ctgccgcttc tctgaggagg cctcactcaa gaggcacacg
     4021 ctgcagaccc acagtgacaa accctacaag tgtgaccgct gccaggcctc cttccgctac
     4081 aagggcaacc tcgccagcca caagaccgtc cataccggtg agaaacccta tcgttgcaac
     4141 atctgtgggg cccagttcaa ccggccagcc aacctgaaaa cccacactcg aattcactct
     4201 ggagagaagc cctacaaatg cgaaacctgc ggagccagat ttgtacaggt ggcccacctc
     4261 cgtgcccatg tgcttatcca cactggtgag aagccctatc cctgtgaaat ctgtggcacc
     4321 cgtttccggc accttcagac tctgaagagc cacctgcgaa tccacacagg agagaaacct
     4381 taccattgtg agaagtgtaa cctgcatttc cgtcacaaaa gccagctgcg acttcacttg
     4441 cgccagaagc atggcgccat caccaacacc aaggtgcaat accgcgtgtc agccactgac
     4501 ctgcctccgg agctccccaa agcctgcttg ccaactttct tgtacaaagt ggttcgatac
     4561 gtctcagctg aagctgctgc aaaggaagct gcagctaagg aggctgcagc taaggctgtg
     4621 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4681 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4741 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4801 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4861 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4921 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4981 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5041 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5101 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5161 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5221 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5281 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaatgcat
     5341 gagtaactga ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     5401 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5461 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5521 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5581 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5641 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     5701 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     5761 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5821 gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     5881 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     5941 aaccctggaa ttcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6001 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     6061 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6121 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6181 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6241 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6301 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6361 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6421 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     6481 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     6541 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     6601 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     6661 gtacaagtaa actagtaagc ttggcgtaac tagatcttga gacaaatggc agtattcatc
     6721 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     6781 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     6841 tttcgggttt attacaggga cagcagagat ccactttggg ctcgaggggg cccgggtgca
     6901 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     6961 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     7021 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     7081 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     7141 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     7201 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     7261 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     7321 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     7381 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     7441 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     7501 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     7561 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     7621 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     7681 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     7741 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     7801 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     7861 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     7921 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     7981 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     8041 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     8101 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac
     8161 ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     8221 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     8281 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     8341 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     8401 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     8461 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     8521 caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg
     8581 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     8641 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     8701 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     8761 tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     8821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     9181 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9301 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9361 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9661 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     9721 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     9781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     9901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10021 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
    10081 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    10141 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    10201 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    10261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10441 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10681 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
    10741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    10801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    10861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    10921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    10981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    11041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    11101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    11161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    11221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    11281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    11341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    11401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    11461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    11521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    11581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    11641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    11701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    11761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    11821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    11881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    11941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    12001 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    12061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    12121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    12181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    12241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    12301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    12361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    12421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    12481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    12541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    12601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    12661 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    12721 tggaaagcgg gcagtgagcg caacgcaat
//
