LOCUS       sequence-338595-       11395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of 1-270AA CycK-GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Slabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, 
            Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, 
            Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, 
            Scholl C, Frohling S, Golub TR, Fischer ES, Thoma NH, Ebert BL
  TITLE     The CDK inhibitor CR8 acts as a molecular glue degrader that 
            depletes cyclin K.
  JOURNAL   Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. 
            Epub 2020 Jun 3.
  PUBMED    32494016
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2374-x"; journalName: "Nature"; date: "2020-09";
            volume: "585"; issue: "7824"; pages: "293-297"
COMMENT     Sequence Label: CycK-GFP (1-270AA) in Cilantro (Codon optimized)
FEATURES             Location/Qualifiers
     source          1..11395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1891..2391
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2310..2328
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3264..3266,3267..3980)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3264..3266,3267..3977)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3306..3327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3567..3586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3914..3935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4004..4578,4579..4581,4582..4590)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4171..4188)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4398..4417
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4600..4609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4606..5316
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4751..4769)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5019..5038
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5372..5489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5623..6801
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5854..6792
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6749..6769
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6814..7413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6814..6833)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7310..7330
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7429..8017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7482..7502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7900..7911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8089..8322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8400..8521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8437..8456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8491..8510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8561..8696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8716..8735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8717..8735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8745..8762)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8745..8761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8754..8776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8903..9358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8990..9009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9200..9221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9384..9488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9489..9557,9558..10349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9707..9726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10520..11108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11009..11028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11262..11279
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc ttgcgccttt tccaaggcag ccctgggttt
     1921 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     1981 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     2041 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     2101 gcgtgccgga cgtgacaaac ggaagccgca cgactcacta gtaccctcgc agacggacag
     2161 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     2221 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     2281 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     2341 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggatcgata
     2401 ccgtcgactc cggaatagcc accatgaagg agaataaaga aaattcaagc ccttcagtaa
     2461 cttcagcaaa cctggaccac acaaagccat gttggtactg ggataagaaa gacttggctc
     2521 atacaccctc acaacttgaa ggacttgatc cagccaccga ggcccggtac cgccgagagg
     2581 gcgctcggtt catctttgat gtgggcacaa gattggggct ccactatgat accctggcaa
     2641 ctggaataat ttattttcat cgcttctata tgtttcattc cttcaagcaa ttcccaagat
     2701 atgtgacagg agcctgttgc ctctttctgg ctgggaaagt agaagaaaca ccaaaaaaat
     2761 gtaaagatat catcaaaaca gctagaagtt tgctcaatga tgtacaattt ggccagtttg
     2821 gagatgaccc aaaggaggaa gtaatggttc tggagagaat cctgctgcag accatcaagt
     2881 ttgatttgca ggtagaacat ccataccagt tcttgcttaa atatgcaaag caactcaaag
     2941 gtgataaaaa caaaattcaa aagttggttc aaatggcatg gacatttgta aatgacagtc
     3001 tctgcaccac cttgtcactg cagtgggaac cagagatcat agcagtagca gtgatgtatc
     3061 tcgcaggacg tttgtgcaaa tttgaaatac aagaatggac ctccaagcct atgtacagga
     3121 gatggtggga acagttcgtt caagatgttc cggtcgacgt cttggaagac atctgccacc
     3181 aaatcctgga tctttactca caaggaaaac aacagatgcc tcatcacacc cccggcggcg
     3241 gcggctccgg cggcggcggc tccgtgagca agggcgagga gctgttcacc ggggtggtgc
     3301 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3361 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3421 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3481 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3541 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3601 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3661 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3721 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3781 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3841 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     3901 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3961 tggacgagct gtacaagtaa atgcatgagt aactgaggat ccgcccctct ccctcccccc
     4021 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4081 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4141 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4201 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4261 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4321 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4381 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4441 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     4501 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4561 gaaaaacacg atgataatat ggccacaacc ctggaattcg ccaccatggt gagcaagggc
     4621 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     4681 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     4741 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     4801 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     4861 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     4921 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     4981 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     5041 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     5101 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     5161 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     5221 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     5281 cactccaccg gcggcatgga cgagctgtac aagtaaacta gtaagcttgg cgtaactaga
     5341 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     5401 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     5461 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     5521 tttgggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     5581 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     5641 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5701 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5761 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5821 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     5881 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     5941 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     6001 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6061 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6121 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6181 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6241 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6301 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6361 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6421 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6481 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6541 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     6601 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6661 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6721 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     6781 cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc cacggtgcgc
     6841 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6901 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6961 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     7021 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     7081 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     7141 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga
     7201 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     7261 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7321 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7381 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg
     7441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7801 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7981 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8041 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8101 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8341 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8521 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8701 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8761 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8821 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8881 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8941 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9241 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9301 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11341 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaat
//
