LOCUS       sequence-338599-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast variant of mitoT synthetic intermembrane tether 
            bridging the inner and outer mitochondrial membranes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Dietz JV, Willoughby MM, Piel RB 3rd, Ross TA, Bohovych I, Addis HG,
            Fox JL, Lanzilotta WN, Dailey HA, Wohlschlegel JA, Reddi AR, Medlock
            AE, Khalimonchuk O
  TITLE     Mitochondrial contact site and cristae organizing system (MICOS) 
            machinery supports heme biosynthesis by enabling optimal performance
            of ferrochelatase.
  JOURNAL   Redox Biol. 2021 Oct;46:102125. doi: 10.1016/j.redox.2021.102125. 
            Epub 2021 Sep 10.
  PUBMED    34517185
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Redox 
            Biol."; date: "2021-10"; volume: "46:102125. doi"; pages: " 
            10.1016/j.redox.2021.102125. Epub 2021 Sep 10"
COMMENT     Sequence Label: pRS423-mitoT
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             409..1119
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELY"
ORIGIN
        1 atgttgaata gttcaagaaa atatgcttgt cgttccctat tcagacaagc gaacgtctca
       61 ataaaaggac tcttttataa tggaggcgca tatcgaagag ggttttcaac gggatgttgt
      121 ttgaggagtg ataacaagga aagcccaagt gcaagacaac cactagatag gctacaacta
      181 ggtgatgaaa tcaatgaacc agagcctatt agaaccaggt tttttcaatt ttccagatgg
      241 aaggccacca ttgctctatt gttgctaagt ggtgggacgt atgcctattt atcaagaaaa
      301 agacgcttgc taaaaccagt tactttgtat gatgttgctg aatatgctgg cctcgctatt
      361 acaacagcca tagcagctct atcagccacc ggttatgcta tctactttat gtctaaaggt
      421 gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt
      481 cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta
      541 aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt cactacttta
      601 acttatggtg ttcaatgttt ttctagatac ccagatcata tgaaacaaca tgactttttc
      661 aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt
      721 aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa
      781 ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac
      841 tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac
      901 ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa
      961 aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt atccactcaa
     1021 tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt agaatttgtt
     1081 actgctgctg gtattaccca tggtattgat gaattgtac
//
