LOCUS       sequence-338605-        1215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3xP3-DsRed fluorescence marker cassette for gene knock-in in 
            mosquitoes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1215)
  TITLE     fluorescence marker cassettes for CRISPR knock-in in mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKSB- 3xP3-DsRedSV40 GGGG AAGA
FEATURES             Location/Qualifiers
     source          1..1215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(270..272,273..275,276..950)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(453..473)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     671..690
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     865..888
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1071..1192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1108..1127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1162..1181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atggtctctg gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg
       61 atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct
      121 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
      181 aaataaacaa gcgcagctga acaagctaaa caatcggggt accgctagag tcgacggtac
      241 cgcgggcccg ggatccaccg gtcgccacca tggtgcgctc ctccaagaac gtcatcaagg
      301 agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg
      361 agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca
      421 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
      481 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
      541 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      601 actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc
      661 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
      721 tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg
      781 gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc
      841 ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca
      901 tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag cggccgcgac
      961 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1021 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1081 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1141 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaagatgag
     1201 accatcaagc ttatc
//
