LOCUS       sequence-338609-        1303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  attP site and 3xP3-GFP fluorescence marker cassette for gene 
            knock-in in mosquitoes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1303)
  TITLE     fluorescence marker cassettes for CRISPR knock-in in mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKSB- attP 3xP3-GFPsv40 GGGG AAGA
FEATURES             Location/Qualifiers
     source          1..1303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      321..330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(327..329,330..332,333..1046)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(327..329,330..332,333..1043)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(372..393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(633..652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     980..1001
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1168..1289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1205..1224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1259..1278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gatggtctct ggggcgaccc tacgccccca actgagagaa ctcaaaggtt accccagttg
       61 gggcacgggg atctaattca attagagact aattcaatta gagctaattc aattaggatc
      121 caagcttatc gatttcgaac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg
      181 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
      241 taaacaagcg cagctgaaca agctaaacaa tcggggtacc gctagagtcg acggtaccgc
      301 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      361 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      421 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      481 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      541 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      601 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      661 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      721 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      781 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      841 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      901 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      961 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1021 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcaaat cagccatacc
     1081 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1141 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1261 ggtttgtcca aactcatcaa tgtatcttaa agatgagacc atc
//
