LOCUS       sequence-338626-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v1
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3952..4540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4005..4025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4423..4434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4593..4714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4604..4623)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4658..4677
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4829..4875
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4905..5041
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5086..5157
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5202..5398
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5249..5384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5412..6206
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5466..5485)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6076..6095
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6217..6441
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6637..7225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6717..6736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7971..8039,8040..8831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8189..8208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8844..8980
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9132..9203
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9208..9254)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9272..9320
                     /note="synthetic polyadenylation signal"
     enhancer        9401..9704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9706..9908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9859..9879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          join(10042..10453,1..64)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc
       61 acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca aaggcggccg
      121 ccatgtttgt gttcctcgtt ctcctgcccc tcgtttcttc ccaatgcgtg aacctcacaa
      181 ctagaacgca gctcccaccc gcttacacga actcttttac tcgtggagta tactatcccg
      241 ataaagtctt taggtccagt gtccttcatt ctactcaaga ccttttcctg ccattcttct
      301 caaatgtcac ctggttccat gctatccacg tttctggcac caacggaact aaacgatttg
      361 ctaatcccgt cttgccattt aacgatggcg tatactttgc ctcaacggag aagtccaaca
      421 ttatccgggg atggatattt ggcaccaccc tggactccaa gacacagtct ctcctcattg
      481 tgaacaacgc caccaatgtc gtcatcaaag tctgtgaatt tcaattttgt aacgatccct
      541 ttctcggggt ttattatcat aagaataaca agtcttggat ggagtcagag ttccgcgttt
      601 acagttccgc taacaactgt acttttgagt acgtttccca accatttctc atggatcttg
      661 aagggaagca aggcaacttc aagaatctcc gcgaatttgt gtttaagaat atagacggct
      721 acttcaaaat ttatagcaag cataccccga taaatctggt tcggggtctc cctcaaggct
      781 tctccgcgtt ggaacccctc gtggacttgc cgattggcat caacatcacc cggttccaaa
      841 cccttcatcg aagctacctc acgcctggcg acagtagtag tggttggaca gcgggcgcag
      901 cagcctacta tgtgggatat ttgcaaccac gcacctttct tctcaaatat aacgaaaacg
      961 gaacaattac cgatgccgta gactgtgccc tcgatcccct gtccgaaact aaatgcacac
     1021 tcaagtcctt cacagttgag aaaggcatat accaaaccag taacttccgc gtccaaccca
     1081 ccgaatccat tgttcgattc cctaacatca ccaatttgtg tcctttcggt gaagtattta
     1141 atgccactcg atttgcatca gtttatgcct ggaatcgcaa gcgaatatca aattgtgttg
     1201 cagattatag tgtcctgtat aatagcgcat cattcagcac ttttaagtgt tatggggtca
     1261 gccctacgaa attgaatgac ttgtgtttta ccaacgttta cgcagattct ttcgtcatac
     1321 gcggcgacga agtccgacag attgcaccgg ggcaaaccgg aaatatagca gactataatt
     1381 ataagttgcc ggatgacttc accggctgcg ttatagcatg gaactccaat aatctcgatt
     1441 caaaagtcgg cggcaactat aattatcttt accgcctgtt tcgcaagtca aatctgaagc
     1501 cctttgaacg tgacatctcc accgaaattt atcaagctgg cagcacccca tgtaatggag
     1561 tgaaagggtt taactgttat tttccccttc aatcatacgg atttcaacca acatatgggg
     1621 tgggctatca accatatcgg gtggtagtct tgagctttga gctgctccac gcaccggcta
     1681 cagtttgcgg accgaagaaa agcactaacc tggtgaagaa taaatgtgtg aacttcaact
     1741 ttaatgggct caccggaaca ggggtgctga cagaatcaaa caagaaattt ctcccttttc
     1801 aacaatttgg tagagacatt gccgatacca cagatgccgt ccgcgatccg caaacactgg
     1861 aaatcctcga tataacaccc tgtagcttcg gcggggtcag cgtgataact cccggaacga
     1921 acacatccaa ccaagtggct gtcctgtatc aaggtgtcaa ctgtaccgaa gtccctgtgg
     1981 cgatacacgc cgaccaactg acacccacct ggagagtcta ctcaacaggt tccaatgtct
     2041 ttcaaactag agcgggttgt ctcattggcg ctgagcatgt caataattca tacgaatgtg
     2101 atattcctat tggggcagga atctgtgcgt cttatcaaac tcaaacgaac tcaccacggc
     2161 gcgctcgctc agtagccagc cagtccataa tcgcctatac catgagtctc ggggttgaga
     2221 actccgtggc ttattcaaac aattccatcg caatcccaac taactttacc attagcgtta
     2281 ccacggaaat cttgcccgtc agcatgacta agacgagcgt agactgtacg atgtatatct
     2341 gtggggattc caccgagtgt agcaacctct tgcttcaata cgggtctttc tgtactcaac
     2401 tcaaccgagc attgaccgga attgcggtcg aacaagacaa gaatacccaa gaggtgttcg
     2461 cacaagtaaa gcaaatatac aaaacgccgc caatcaaaga ttttggcggg tttaatttta
     2521 gccaaatact gccagaccca tctaagcctt caaaacgcag ctttatcgag gatctgctgt
     2581 tcaacaaagt tacgttggct gacgcgggtt tcattaagca gtatggggat tgtttgggag
     2641 acattgcggc ccgcgacctt atctgtgctc aaaaattcaa tgggttgaca gttctgcccc
     2701 ctctgctcac tgatgagatg attgcacagt atacatcagc attgttggca ggaaccatta
     2761 catccggctg gacttttggc gcgggtgcgg cccttcaaat acctttcgct atgcaaatgg
     2821 cttacagatt caatgggatt ggtgtcacgc aaaatgtatt gtatgaaaat cagaagctga
     2881 tcgctaatca atttaacagc gcgattggca agatccaaga ttcactgtca agcacagcgt
     2941 ccgccctcgg caaactccaa gatgttgtga atcaaaacgc ccaggccctg aataccctcg
     3001 tgaagcaact gagttctaac ttcggcgcaa tctccagcgt cctcaacgac atattgtcca
     3061 gacttgataa ggtggaagcc gaggtgcaaa ttgatcggct catcacaggc aggctccagt
     3121 cactccaaac ttatgtgaca caacagttga tccgggctgc tgagatacga gcaagcgcca
     3181 acctggccgc cactaaaatg tcagagtgcg tgctcgggca gtccaaacgg gttgactttt
     3241 gtggcaaggg ctatcacttg atgtccttcc cccaatccgc cccacatgga gtggtctttc
     3301 tccacgtcac ttatgtccct gctcaagaga agaactttac gacagctcca gcaatctgtc
     3361 acgatggcaa agcgcacttt cccagggaag gcgttttcgt cagtaatggc acgcattggt
     3421 ttgtgaccca aaggaatttc tatgaacccc aaatcataac cactgacaat acatttgtct
     3481 caggcaactg tgatgtggtc attgggatcg tgaacaacac ggtatatgat cccctgcaac
     3541 ccgagttgga tagcttcaaa gaggaattgg ataaatactt taagaatcat acttcacccg
     3601 atgttgatct cggggacata tcaggaataa atgcaagtgt cgtcaacatt cagaaagaga
     3661 ttgatcgcct taatgaggtc gccaagaact tgaatgaatc tttgatcgac ttgcaagaac
     3721 tgggcaaata cgaacaatat attaaatggc cctggtacat ttggttgggc tttatcgccg
     3781 gacttatagc tatcgttatg gtcacaatta tgttgtgctg tatgactagc tgttgttcct
     3841 gcttgaaagg atgttgcagt tgcggatctt gttgtaaata aaggatcctc tagaagttgt
     3901 ctcctcctgc actgactgac tgatacaatc gatttctgga tccgcaggcc taatcaacct
     3961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4321 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4501 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtctagct tgactgactg
     4561 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac
     4621 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4681 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4741 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4801 tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc
     4861 ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa
     4921 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     4981 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     5041 aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt
     5101 cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc
     5161 tcattggtta ccttgggcta tcgaaactta attaagcgat ctgcatctca attagtcagc
     5221 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5281 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg
     5401 aggtagccaa catgattgaa caagatggat tgcacgcagg ttctcccgcc gcttgggtgg
     5461 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5521 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5581 tgaatgaact ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5641 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5701 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5761 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5821 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5881 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5941 ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg ccgaatatca
     6001 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6061 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6121 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6181 atcgccttct tgacgagttc ttctagtatg taagccctgt gccttctagt tgccagccat
     6241 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6301 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6361 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6421 gggatgcggt gggctctatg gttaattaac cagtcaagtc agctacttgg cgagatcgac
     6481 ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg gtccttgcta
     6541 ttgcacccgt tctccgatta cgagtttcat ttaaatcatg tgagcaaaag gccagcaaaa
     6601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7441 atttcgttca tccatagttg catttaaatt tccgaactct ccaaggccct cgtcggaaaa
     7501 tcttcaaacc tttcgtccga tccatcttgc aggctacctc tcgaacgaac tatcgcaagt
     7561 ctcttggccg gccttgcgcc ttggctattg cttggcagcg cctatcgcca ggtattactc
     7621 caatcccgaa tatccgagat cgggatcacc cgagagaagt tcaacctaca tcctcaatcc
     7681 cgatctatcc gagatccgag gaatatcgaa atcggggcgc gcctggtgta ccgagaacga
     7741 tcctctcagt gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc
     7801 cagcttggga cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc
     7861 gtaccgatct gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc
     7921 tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc atgagtattc
     7981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcg cgagtgggtt
     8101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgct
     8161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt
     8281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga
     8401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8521 tggcaacaac cttgcgtaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8581 agttgataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8821 agcattggta accgattcta ggtgcattgg cgcagaaaaa aatgcctgat gcgacgctgc
     8881 gcgtcttata ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc
     8941 acttccatac gtgtcctcct taccagaaat ttatccttaa gatcccgaat cgtttaaact
     9001 cgactctggc tctatcgaat ctccgtcgtt tcgagcttac gcgaacagcc gtggcgctca
     9061 tttgctcgtc gggcatcgaa tctcgtcagc tatcgtcagc ttaccttttt ggcagcgatc
     9121 gcggctcccg acatcttgga ccattagctc cacaggtatc ttcttccctc tagtggtcat
     9181 aacagcagct tcagctacct ctcaattcaa aaaacccctc aagacccgtt tagaggcccc
     9241 aaggggttat gctatcaatc gttgcgttac acacacaaaa aaccaacaca catccatctt
     9301 cgatggatag cgattttatt atctaactgc tgatcgagtg tagccagatc tagtaatcaa
     9361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9661 ctacttggca gtacatctac gtattagtca tcgctattac catgctgatg cggttttggc
     9721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     9781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     9841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     9901 gcagagctgg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     9961 tccatagaag acaccgggac cgatccagcc tcccctcgaa gcttacatgt ggtaccgagc
    10021 tcggatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct
    10081 tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca catattgacc
    10141 aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt
    10201 tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac
    10261 aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg
    10321 caatagcaat atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg
    10381 tttcatattg ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg
    10441 gataaggctg gat
//
