LOCUS       sequence-338642-       10632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Utilisation of pSAM2 excision tool in Amycolatopsis spp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Santos LDF, Caraty-Philippe L, Darbon E, Pernodet JL
  TITLE     Marker-Free Genome Engineering in Amycolatopsis Using the pSAM2 
            Site-Specific Recombination System.
  JOURNAL   Microorganisms. 2022 Apr 16;10(4). pii: microorganisms10040828. doi:
            10.3390/microorganisms10040828.
  PUBMED    35456877
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microorganisms."; date: "2022-04-16"; volume: "10(4). pii: 
            microorganisms10040828. doi"; pages: " 
            10.3390/microorganisms10040828"
COMMENT     Sequence Label: pEA01
FEATURES             Location/Qualifiers
     source          1..10632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             419..1213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(473..492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1083..1102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1573..1592
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1844..2432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1924..1943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5767..6366
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5767..5786)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6263..6283
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     oriT            6560..6669
                     /note="incP origin of transfer"
     CDS             6702..7073
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     complement(8645..8667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8656..8672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8680..8686,8687..8703,8704..8709))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cgcccggtgc gagttcgacc ttctggtacc gagctcgaat tcagatcctt taaagcttca
       61 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
      121 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
      181 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
      241 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
      301 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
      361 cgcaggggat caagatcccc ggggatctga tcaagagaca ggatgaggat cgtttcgcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     1261 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1321 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1381 caccccgggc tcgatcccct cgcgagttgg ttcagctgct gcctgaggct ggacgacctc
     1441 gcggagttct accggcagtg caaatccgtc ggcatccagg aaaccagcag cggctatccg
     1501 cgcatccatg cccccgaact gcaggagtgg ggaggcacga tggccgcttt ggtcgatact
     1561 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     1621 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     1681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2521 ggatcttcac ctagccaccc gatgagccgc cgccgcgccg tccagcgagg agcccgccgg
     2581 gccgagcccg tcgacccgca cgcgtccatc gagggccagc tggccctgtt cggcgacgac
     2641 ccgcagcccg agcccgtcaa ggccgagctg gccgccgtcg acccggcgga ggcgtcccga
     2701 tgaacccgcg gaacaacgcc gacgacgcca gcgacgccgc cgtgggctgg accgaggccc
     2761 ggtggctggt ctccaacggc aaggcgaagt agtcctgacc gtcgaccagg tggtgcccgc
     2821 cgggccgttg cccggcgggc actgtcgcgt tcggatgcac ggagaccagg ggaaacgtgg
     2881 acgtctgacg atgatcggcc gtaggatcac ggacgcgccc gacgcgcaaa cggtccgcgt
     2941 cggccgcgag aggcgggtta cccccggccc gccaccctct cgcgtggcat tcgcccgaac
     3001 gtggcaactt ttccggcgtg atcatcggcg tgtcgctcgt tttcgcgtac tcgaaacgat
     3061 agaacgtgcc gtatgggacg accgaagggc agacccccgc gctgggacgc cgacgttcag
     3121 ctgaaggtga tcaaggcccg gatgccggtc gagctggccg aggcgttcca gaaggaagcg
     3181 cagaagcgcg gcatgaacgt gcaggacttc ctgggccgac tggccgagga gctgaccggc
     3241 gtgccctaca acccgcaggg aggtttgccg ctgagcgcct agaacgacga aaagcgcccc
     3301 gccaggagtt accagctccc gcgagacgct tccacgccgc taactgaaca tccacgccca
     3361 ccaaggccac tgactcgtga agggtttcgc cggccgttgg tgcgctgtat ctacgcttgg
     3421 agccaccata ccaacccggc ccaggtgtgg cccagtactg cccgcgtgcg acacgcggac
     3481 ggattctcca gggtccaccg aatcagcaat cttgacgctt aagcgtcaac ctgtcagcga
     3541 cttgtgcgtc agttgcggcc gggcccctgg cgtgccacgg cccaccccgt tgcggaccgt
     3601 ccactgtggc ccgtgctggg ccgagttcgg cgggtcgccc gacgtcgccg agatcggccc
     3661 gcccccgccg gaggctctgg ggtcgcgcga ccccagagac cagcgggaat ggataacgag
     3721 cctggccggt gagcagtggg tccagatgct ccggagcgac tgccgcgccc gtctgctcga
     3781 cctcgcacag gagatgatgc tccgcgcgaa ctgggacacc tgggagtcgt ggccaggctg
     3841 ggaccggctc atggaggtgt cccgctggaa gcgctccgcg ctggccggct ggctgcgcca
     3901 gctgcgcctg ctcggctggt tgtcagtgat cgagtccggc agcacgccgc agttccggcc
     3961 gatggtcctg tcccacgtcg agggcaaccg ccgcgccgtc tacgccctgc gggtgccccg
     4021 ccgtcccggc gagcaggcca ccgcccccca accgccagcc cgtcccgagc ccgagctgga
     4081 gaccgtcccg gccgagccgt tcacccgttc tgaccagcgc ggacgcgcgt ctgtggatag
     4141 aacctggacc cctacgtggt tctttgatct ttcttctaga gttgatcttt gggtttcttc
     4201 gcgcgcgaga gattttttcc acagctccca gctcggcgcg caggttgagc ccaagacgga
     4261 gccgcttcgc ggccgctttg acgaggaatg gctggcgttc gctcgaacgt cgccgaccag
     4321 cggcgctcag atgctcgccg cggccgccga gctgcgccgc caggagccga tcctggccca
     4381 gttgaccgcg cgcgccgtcc gggccctgtg tcgcccgtac tgggctgctg gctgggcaaa
     4441 cgacgatgtg cggcacgcgc tccgctggcg gccgcgggcc acgtcgagcc tgcccgcgat
     4501 ggagcaggcg aagatccacg cgccgtaccg gtgggcgcag tcgaggctgg ccgcctggcg
     4561 caccgacacc ggcaaggttc tgcctggtca cagccacatg gagcgcgccc agcggacccg
     4621 cgagcacgcg gtggtggccc gcttcggcaa ggccgcgctg gccgcgatgc ccgccgggtc
     4681 ggaccggctg cgcccggttg acgtgcgccg gttcggccgc cgccgggtcg acgccgcggt
     4741 ggagacgctg cgccgccgtc gggccgccga gctggccgac gagcgcgccg ccgtccggtc
     4801 gcaggccgag gccgccgacc ccgagcacgc ccgccgggtc gccgacgagt tccgcgccga
     4861 gcgcgagcgc cgccaggccg ctggccgcga gcggctgctg gagcaggccc gcgccgcggc
     4921 cgccgagcgg gccgagcagg accagcccgc cccggcgagc acgtggacac cgcagggacc
     4981 agcgcaaacg ccggaggagc ggcaggaggc cgcgctggcc cgagcacggg cggaaggccg
     5041 cgtgcccctg gtccgtcgtc gccgccggac ccgttggtga tgcttgcggc gctggcctgg
     5101 gtggtaccag ggggctcgcg ccccctggac cccccggccc agcggcacga cccgcgccgg
     5161 acgttccagc ggtgccggtt ccgttggcgc gggccgcacc gctggagtgg tgccgagggt
     5221 ccggtgagaa ggtggtcacc agcccgtcga ccagcaggag cgcgtccgca tgaccagccc
     5281 cgatgacctg gacgactacc tgcgggaact actgcgcagg gagaaggccg gcgagcggca
     5341 ccggatcgag gttggcccgt acacggcgat gctggtcacg ggggcgatcc agtgggcgat
     5401 ccggcgcggt gagctggccg agcacgggcc ggtgatgttc gagcggctgc tggaccagct
     5461 ccggctgctg ttcgccgacg acccgagggg cgcggagctg atctatatct atgatctcgc
     5521 agtctccggc gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca
     5581 tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc
     5641 cgcagtggct ctctatacaa agttgggcat acgggaagaa gtgatgcact ttgatggccg
     5701 ccacgaccgg tgccgccacc atcccctgac ccacgcccct gacccctcac aaggagacga
     5761 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     5821 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     5881 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5941 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6001 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6061 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6121 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6181 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6241 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6301 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6361 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgac
     6421 ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     6481 catcctttct cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     6541 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     6601 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     6661 gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc
     6721 caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga
     6781 ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc
     6841 agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca
     6901 atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca
     6961 cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg
     7021 acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt
     7081 agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc
     7141 aagaagagcg acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgat
     7201 gatagcttgc atgcctgcag gtcgactcta gatttctgtg cgtgtctatc tgcgtgacta
     7261 tcgcgccgga ccccgcttga agatgccgtc catgaccaca gcgccggtct ggatgacggg
     7321 ccggatctgc ttccggtaga cctcctcagt cacggccgta ccggagtgtc cgacgagccg
     7381 ggagatctcc tccagcggga cgccgcggtc ggacagcagg gacacgaagc tgtgcctcag
     7441 ctccctcggt gtccactcgt cggcgttgat cccgttggca tccttgagcg cctggcggaa
     7501 ggcgcgccgg acgttagtcg cgtcgagcgg cttgccaacg gccgacgaga agaccaggcc
     7561 gtgttcctcc cacttgtcac cggcggcgag ccgttcccag ccctggtcct caaagtgctg
     7621 ccagaggacc tccacgcaac gcgccggcag ggcgagcgtt cgccgagact tccgggtttt
     7681 cgtgtcccca ccgcgccgga ccgagcgcca gacggcgatg tgcggaggct gcggcggctc
     7741 aacgtccgga cttcccttga ggaagacgtg gtcccaggtc agcgcccgca gctcctcggt
     7801 gcgcgcaccg gtcagcaggg cgacgacgat gtaggcgtgc atcgacgtgc cctcggcagc
     7861 attcagcacc gcctcggcct gggcgaaggt gagcgccttg gacggccggc caggctggcc
     7921 ctggggcaca gagcacagct ccaccacgtt gcgcttcacc ttgtcacgcg ccatggcccg
     7981 cttgaccgcc cggttcaggc aggagtggac cgcctgaagg ctgcgcgtgc tcagagtctg
     8041 agccttggcg gccagccagc ggtcgacgtc ctctgcgctg aggtcacgca gcttccgggc
     8101 acccaaaccc ggtatgacgt gcttctggct taggtgggtg cagttctcga cggtgcgctg
     8161 gtcacggcca gcgagaccgt aggcaagcca gtcgttcacc gcgtcggcga cggtgtaccc
     8221 cgtgggtgcg atcgcgagac cgtcttcgtg gtcacgcaga acctctttga gcttgttctt
     8281 ggcctccgtc ttggtcttgc cactcccccg cttgacgatc cgcttaccgc tcggatcgaa
     8341 gccgaggttc gccgtggcga tccagcgctg tctcttctcg tcccagtgga ggccgccgtc
     8401 accccggcta cgtcgcttgg ccatcagatg gcctctccca actggtcctg aatgaagtcg
     8461 cgtacggcgt cggtgggtac tcgacgcgct ccgtcgatct tgatggaggc aaggcggcgg
     8521 gtgcggatga ggtcgtagac cttgctgcgt ccaaccttca gccgcgccat gacttccggc
     8581 acggtcagca gctctggagt gacggtggtc atggtacctt cctcccgaat tccatggtct
     8641 gtttcctgtg tgaaattgtt atccgctcac aattccacac attatacgag ccggatgatt
     8701 aattgtcaac agctcatttc agaatatcgt ccattccgac agcatcgcca gtcactatgg
     8761 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     8821 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     8881 ctacgcgatc atggcgacca cacccgtcct gtggatctag aattcgagct cggtacccgg
     8941 ggatctgcag ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccagc
     9001 ccgacccgag cacgcgccgg cacgcctggt cgatgtcgga ccggagttcg aggtacgcgg
     9061 cttgcaggtc caggaagggg acgtccatgc gagtgtccgt tcgagtggcg gcttgcgccc
     9121 gatgctagtc gcggttgatc ggcgatcgca ggtgcacgcg gtcgatcttg acggctggcg
     9181 agaggtgcgg ggaggatctg accgacgcgg tccacacgtg gcaccgcgat gctgttgtgg
     9241 gctggacaat cgtgccggtt ggtaggatcc agcggtgagc agttcggacg agcagccgcg
     9301 cccgcgtcgc cgcaaccagg atcggcagca ccccaaccag aaccggccgg tgctgggccg
     9361 taccgagcgg gaccgcaacc ggcgccagtt cgggcagaac ttcctccgcg accgcaagac
     9421 catcgcgcgc atcgccgaga cagccgagct gcggcccgat ctgccggtgc tggaagccgg
     9481 ccccggcgaa gggctgctca ccagggaact cgccgaccgc gcgcgtcagg tgacgtcgta
     9541 cgagatcgac ccccggctgg cgaagtcgtt gcgggagaag ctttccggcc acccgaacat
     9601 cgaagtcgtc aacgccgact tcctcaccgc cgaaccgccg cccgagccgt tcgccttcgt
     9661 cggcgcgatc ccctacggca tcacctcggc gatcgtggac tggtgcctgg aggcgccgac
     9721 gatcgagacg gcgacgatgg tcacgcagct ggagttcgcc cggaagcgga ccggcgatta
     9781 cggccgctgg agccgcctca cggtgatgac ctggccgctg ttcgagtggg agttcgtcga
     9841 gaaggtcgac cgccggctgt tcaagccggt gcccaaggtc gactcggcga tcatgcggct
     9901 gcgcaggcgc gccgaaccgc tgctggaagg cgcggcgctc gaacgctacg agtcgatggt
     9961 cgagctgtgc ttcaccggcg tcggcggcaa catccaggcg tcgcttctgc gcaagtaccc
    10021 gaggcgccgc gtcgaggcgg cgctcgacca cgcgggggtc gggggcggcg ccgtggtcgc
    10081 ctacgtccgg ccggagcagt ggctccggct gttcgagcgg ctggatcaga agaacgaacc
    10141 gaggggtggg cagccccagc ggggcaggcg aaccggcgga cgggaccacg gggaccggcg
    10201 aaccggcggg caggatcgcg gcgatcggcg aaccggcggc cgcgaccaca gggaccggca
    10261 agccagcggc cacggcgatc gtcgcagcag cggacgcaat cgcgacgacg gacgaaccgg
    10321 cgagcgcgag cagggggacc aaggcgggcg gcgggggccg tccgggggtg gacggaccgg
    10381 cggacgtcca gggcgacgcg gcggacccgg gcagcggtag tccccggcac gcggaacggg
    10441 gcaggccgtc gagcggcctg ccccgttctg tcgagaggaa tcagaggttg atgtcggccc
    10501 ggaggtcgat gtcgcgcgac gacgagccga tctccaccgc tcgcttgccg cccccgagct
    10561 tccagccgcc cgcggcttcg tcccagtgct ggagggcccg ctccgcgacg tgcacccgga
    10621 cgcgcttggt ct
//
