LOCUS       sequence-338646-        3106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector carrying the site attR of the SSR system of pSAM2. 
            This plasmid is integrated into the genome of Actinobacteria by HR 
            when carrying a DNA fragment identical to a region of the genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Santos LDF, Caraty-Philippe L, Darbon E, Pernodet JL
  TITLE     Marker-Free Genome Engineering in Amycolatopsis Using the pSAM2 
            Site-Specific Recombination System.
  JOURNAL   Microorganisms. 2022 Apr 16;10(4). pii: microorganisms10040828. doi:
            10.3390/microorganisms10040828.
  PUBMED    35456877
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microorganisms."; date: "2022-04-16"; volume: "10(4). pii: 
            microorganisms10040828. doi"; pages: " 
            10.3390/microorganisms10040828"
COMMENT     Sequence Label: pEA03
FEATURES             Location/Qualifiers
     source          1..3106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    284..317
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            518..627
                     /note="incP origin of transfer"
     primer_bind     complement(2281..2298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2415..2960)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2495..2514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3061..3094
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aggtaagctt gatatcgcgc gcttcgttcg ggacgaagag gtcgtgggtt caaatcccgc
       61 caccccgaca gagaaacacc aggtgaggca gaccgtaacg ttacggtctg cctcacctgt
      121 tttctgtgcg tgtctatctg cgtgactatc gcgccggacc ccgcttgaag atgccgtcca
      181 tgaccacagc gccggtctgg atgacgggcc ggatctgctt ccggtagacc tcctcagtca
      241 cggccgtacc ggagtgtccg acgagccggg agatctctgc agggaagttc ctattctcta
      301 gaaagtatag gaacttcgtc cacgacgccc gtgattttgt agccctggcc gacggccagc
      361 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
      421 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
      481 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
      541 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
      601 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
      661 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
      721 atcgctataa tgaccccgaa gcagggttat gcagcggaaa agggtaccac gtcgcggtga
      781 gttcaggctt tttcatatct cattgccccc ggacgagcgt ctgctccgcc attcgccgtc
      841 cgccgtgcca atcggatcag ccgtccaaat gcgggatttt cgttagtcgg aggccaaacg
      901 gcattgagcg tcagcatatc atcagcgagc tgaagaaaga caatccccga tccgctccac
      961 gtgttgcccc agcaatcagc gcgaccttgc ccctccaacg tcatctcgtt ctccgctcat
     1021 gagctcaggc gccgggggcg gtgtccggcg gcccccagag gaactgcgcc agttcctccg
     1081 gatcggtgaa gccggagaga tccagcgggg tctcctcgaa cacctcgaag tcgtgcagga
     1141 aggtgaaggc gagcagttcg cgggcgaagt cctcggtccg cttccactgc gccccgtcga
     1201 gcagcgcggc caggatctcg cggtcgcccc ggaaggcgtt gagatgcagt tgcaccaggc
     1261 tgtagcggga gtctcccgca tagacgtcgg tgaagtcgac gatcccggtg acctcggtcg
     1321 cggccaggtc cacgaagatg ttggtcccgt gcaggtcgcc gtggacgaac cggggttcgc
     1381 ggccggccag cagcgtgtcc acgtccggca gccagtcctc caggcggtcc agcagccggg
     1441 gcgagaggta gccccacccg cggtggtcct cgacggtcgc cgcgcggcgt tcccgcagca
     1501 gttccgggaa gacctcggaa tggggggtga gcacggtgtt cccggtcagc ggcaccctgt
     1561 gcagccggcc gagcacccgg ccgagttcgc gggccagggc gagcagcgcg ttccggtcgg
     1621 tcgtgccgtc catcgcggac cgccaggtgg tgccggtcat ccggctcatc accaggtagg
     1681 gccacggcca ggctccggtg ccgggccgca gctcgccgcg gccgaggagg cggggcaccg
     1741 gcaccggggc gtccgccagg accgcgtacg cctccgactc cgacgcgagg ctctccggac
     1801 cgcaccagtg ctcgccgaac agcttgatca ccgggccggg ctcgccgacc agtacggggt
     1861 tggtgctctc gccgggcacc cgcagcaccg gcggcaccgg cagcccgagc tcctccaggg
     1921 ctcggcgggc cagcggctcc cagaattcct ggtcgttccg caggctcgcg taggaatcat
     1981 ccgaatcaat acggtcgaga agtaacaggg attcttgtgt cacgacattg cactccaccg
     2041 ctgatgacat cagtcgatca tagcacgatc aacggcactg ttgcaaatag tcggtggtga
     2101 taaacttatc atcccctttt gctgatggag ctgcacatga accggatccc ctagcggagt
     2161 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     2221 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     2281 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     2341 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     2401 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     2461 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     2521 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     2581 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     2641 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     2701 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     2761 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     2821 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     2881 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     2941 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     3001 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3061 gaagttccta ttctctagaa agtataggaa cttcatgcat atacct
//
