LOCUS       sequence-338664-        3178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-A-SP44-mScarlet-I
FEATURES             Location/Qualifiers
     source          1..3178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..60
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(64..107)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    110..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     835..858
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             942..965
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             966..983
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     terminator      1110..1181
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1197..1224
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1257..1274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1428..2016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1508..1527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2187..2978,2979..3047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2810..2829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3048..3152)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
       61 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      121 cgcttctaga acgtctcaat ctctattgtt cacattcgaa ccgtctctgc tttgacaaca
      181 tgctgtgcgg tgttgtaaag tctggtgtag tactttaact ttaagaagga gatatacata
      241 tggtgtccaa gggcgaggcc gtcatcaagg agttcatgcg cttcaaggtc cacatggagg
      301 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccgtacgagg
      361 gcacccagac cgccaagctg aaggtcacca agggcggccc gctgccgttc tcctgggaca
      421 tcctgtcccc gcagttcatg tacggctccc gcgccttcat caagcacccg gccgacatcc
      481 cggactacta caagcagtcc ttcccggagg gcttcaagtg ggagcgcgtc atgaacttcg
      541 aggacggcgg cgccgtcacc gtcacccagg acacctccct ggaggacggc accctgatct
      601 acaaggtcaa gctgcgcggc accaacttcc cgccggacgg cccggtcatg cagaagaaga
      661 ccatgggctg ggaggcctcc accgagcgcc tgtacccgga ggacggcgtc ctgaagggcg
      721 acatcaagat ggccctgcgc ctgaaggacg gcggccgcta cctggccgac ttcaagacca
      781 cctacaaggc caagaagccg gtccagatgc cgggcgccta caacgtcgac cgcaagctgg
      841 acatcacctc ccacaacgag gactacaccg tcgtcgagca gtacgagcgc tccgagggcc
      901 gccactccac cggcggcatg gacgagctgt acaagggatc cgactacaag gacgacgacg
      961 acaagtgctg cccgggctgc tgctaattgc catgtgtatg tgggagacgg tcgggtccag
     1021 atattcgtat ctgtcgagta gagtgtgggc tcccacatac tctgatgatc cttcgggatc
     1081 attcatggca aagatcctcg accaggcatc aaataaaacg aaaggctcag tcgaaagact
     1141 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1201 caccttcggg tgggcctttc tgcgtttata tgtttgccag agacgctgca ggcttcctcg
     1261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
