LOCUS       sequence-338665-       10206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. 
            Enhancer grammar GB20 entry point for custom enhancers. 
            Cut-and-paste cloning can be used. Purple-white bacteria colony 
            screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pPuroR-MCS-GAL4-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..190
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        218..669
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     617..638
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        1423..1441
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1423..1440
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1452..1469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2277..2371
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2378..2434
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(2496..2514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2677..3117
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    3518..3652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3542..3561)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3596..3615
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3740..3758)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4210..4281,4683..4956,5031..5685,5747..6062,6266..6397,
                     6468..7082)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     rep_origin      complement(7849..8437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7929..7948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8905..9696
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9751..10179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9838..9857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10048..10069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgcagctgc ccgggtctag agtcgacgca tgcctcctga ctcgacgatt
       61 tacgggaaat gaagctctcc ggatcccagg gaggaattgg tcgacgatgt aggtcactgt
      121 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      181 ctcacccatc tggtccatca tgatgcgctg tcaggagcta gaatcccaaa acaaactggt
      241 tattgtggta ggtcatttgt ttggcagaaa gaaaactcga gaaatttctc tggccgttat
      301 tcgttattct ctcttttctt tttgggtcgc tccctctctg cactaatgct ctctcactct
      361 gtcacacagt aaacggcata ctgctctcgt tggttcgaga gagcgcgcct cgaatgttcg
      421 cgaaaagagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      481 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      541 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      601 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
      661 gagaactctc aatactcgaa tccatgtggg agtttattct tgacacagat atttatgata
      721 taataactga gtaagcttaa cataaggagg aaaacaaaat gaccgagtat aagcccacgg
      781 tgcgtctggc cacccgtgat gatgtgcccc gtgccgtgcg aaccctggct gctgccttcg
      841 ccgattaccc agccacccgt cacaccgtgg acccagaccg tcacattgag cgtgtgaccg
      901 agctccagga gctgttcctg acacgcgtgg gcctggatat cggcaaggtc tgggtggccg
      961 atgatggagc tgccgtggcc gtgtggacca cccccgagag cgtggaggct ggagccgtgt
     1021 tcgccgagat tggaccccgt atggccgagc tgagtggctc ccgtctggct gcccagcagc
     1081 agatggaggg cctgctggcc ccacaccgtc caaaggagcc cgtctggttc ctggccaccg
     1141 tgggcgtgag ccccgaccac cagggcaagg gcctgggcag tgccgtggtg ctgccaggag
     1201 tggaagctgc cgagcgtgct ggagtgccag ccttcctgga aaccagtgcc ccacgcaatc
     1261 tgcccttcta cgagcgtctg ggcttcaccg tgacggccga tgtggaggtg cccgagggcc
     1321 cacgcacctg gtgtatgacc cgtaagccag gagcctaagc ttggcaataa aaagacagaa
     1381 taaaacgcac gggtgttggg tcgtttgttc cgctgtcagg agatttaggt gacactatag
     1441 acttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgtc gggagagaga
     1501 ccaatccatg tgggagttta ttcttgacac agatatttat gatataataa ctgagtaagc
     1561 ttaacataag gaggaaaaca aaatggcgag cttggttaag aaagatatgt gtgttaagat
     1621 gacgatggaa ggtactgtga acggttatca ctttaagtgc gttggcgagg gtgaaggcaa
     1681 gccgttcgag ggcacgcaga acatgcgcat tcgtgtcacc gagggcggtc cgctgccttt
     1741 tgcattcgac atcctggccc cgtgctgtat gtacggctct aagaccttca ttaaacacgt
     1801 gagcggtatc ccggattact ttaaagagtc ctttccagag ggcttcactt gggaacgtac
     1861 ccagattttt gaggacggtg gtgttctgac cgcgcaccaa gacaccagcc tggaaggtaa
     1921 ttgcctgatc tataaagtga aggttctggg taccaatttc ccggcgaatg gtccggtgat
     1981 gcaaaagaaa accgcgggtt gggagccgtg cgtcgagatg ctgtatccgc gtgacggcgt
     2041 cttgtgtggt cagagcttga tggcgctgaa gtgcaccgat ggcaatcatc tgaccagcca
     2101 cctgcgcacg acgtatcgta gccgtaaacc gagcaacgcc gttaacatgc cggagttcca
     2161 ttttggtgac catcgcatcg aaatcctgaa agctgagcag ggcaaattct acgaacaata
     2221 cgaatcggct gtcgcacgtt acagcgatgt gccggaaaaa gcgacgtaat gagcttgact
     2281 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     2341 ttgccgccgg gcgtttttta ttggtgagaa ttacttcaag cttgcatgcc tgcaggtcga
     2401 ctctagagga tccccgggta ccgagctcga attcggtctc ttcccgcttg tgtgaaatac
     2461 cgcacagatg cgtaaggaga aaataccgca tcaggcctat agtgagtcgt attatcccga
     2521 gctcgcccgg ggatcgagcg cagcggtata aaagggcgcg gggtggctga gagcatcagt
     2581 tgtgaatgaa tgttcgagcc gagcagacgt gccgctgcct tcgttaatat cctttgaata
     2641 agccaacttt gaatcacaag acgcatacca aaccaaatga agctgctgag tagtattgaa
     2701 caagcgtgtg acatatgccg acttaagaag ctgaagtgca gcaaggaaaa gccgaagtgc
     2761 gccaaatgtc ttaagaacaa ttgggagtgc cggtactcgc caaagacgaa gcgaagccct
     2821 ttgacacgcg ctcatttgac tgaagtggag agtcgcctgg agcgccttga gcagttgttc
     2881 ctcctcattt ttccccgtga agacctcgac atgatactga agatggatag tctccaagat
     2941 attaaggccc tgctcactgg cctgtttgtt caggataacg tcaacaagga tgccgtgacg
     3001 gaccgccttg cctcggtgga gacagatatg ccacttacct tgcggcagca tcgcatctcg
     3061 gctaccagca gtagtgaaga aagttccaat aagggtcaac ggcaactgac ggtatcgagt
     3121 ggcggaggtt ccggtggcgg cagcggcggg ggttcgggag gtggctcggc caacttcaac
     3181 cagagtggta acattgcgga cagctccttg agcttcacgt ttaccaacag ctccaacggt
     3241 ccaaacttga tcacgactca gacaaactcc caggcattgt cccagcccat tgccagctcg
     3301 aatgtgcatg ataactttat gaataatgaa attactgcct ccaagattga cgacggcaac
     3361 aacagcaagc ccctttcccc aggctggact gaccagacgg cctacaatgc tttcggtatc
     3421 accactggca tgttcaacac cacaacgatg gacgacgtat ataactatct ttttgatgat
     3481 gaggatactc caccgaaccc aaagaaggag taggcttgat ccagacatga taagatacat
     3541 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3601 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3661 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcgctgtc
     3721 aggaggaatt ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa
     3781 aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa
     3841 tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa
     3901 tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg
     3961 tcactttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc
     4021 aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac
     4081 gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt
     4141 tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa
     4201 atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca
     4261 tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat
     4321 ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa
     4381 aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg
     4441 ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc
     4501 attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct
     4561 agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt
     4621 ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc
     4681 agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa
     4741 actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac
     4801 tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc
     4861 cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac
     4921 acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct
     4981 gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt
     5041 ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct
     5101 gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg
     5161 actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca
     5221 gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat
     5281 ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat
     5341 ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa
     5401 aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc
     5461 gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt
     5521 cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca
     5581 gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt
     5641 agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt
     5701 ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt
     5761 gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac
     5821 gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag
     5881 tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc
     5941 cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc
     6001 gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa
     6061 cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc
     6121 gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg
     6181 tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag
     6241 atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg
     6301 ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac
     6361 caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt
     6421 tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag
     6481 agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc
     6541 tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga
     6601 ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc
     6661 tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct
     6721 cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg
     6781 gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct
     6841 catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg
     6901 gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg
     6961 agacacttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca
     7021 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
     7081 agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt
     7141 ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag
     7201 atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat
     7261 ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg
     7321 ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt
     7381 cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct
     7441 ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc
     7501 gattcggttc gctcaaatgg ttccgagtgg ttcatttcgg ctcaatagaa attagtaata
     7561 aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta
     7621 tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt
     7681 gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca
     7741 tcgcttgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8461 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8521 aaaaaggatc ttcacctaga tccttttgtg accagccagg acagaaatgc ctcgacttcg
     8581 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     8641 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     8701 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     8761 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     8821 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     8881 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
     8941 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     9001 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     9061 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     9121 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     9181 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     9241 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     9301 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     9361 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     9421 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     9481 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtagctgc cgactgggca
     9541 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     9601 ggacaagaag aagatcgttt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     9661 gtgaaaggcg agatcaccaa ggtagtcggc aaataatcac tagaccaatg ttacacatat
     9721 atactttaga ttgatttaaa acttcatgtt acgcgccctg tagcggcgca ttaagcgcgg
     9781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9961 ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt tttcgccctt
    10021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    10081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    10141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    10201 caattt
//
