LOCUS       sequence-338667-        9704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLyGo cloning vector for a sequence of interest (LPMO) in K. 
            phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo 
            cassette (SapI-ccdB-SapI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Hernandez-Rollan C, Falkenberg KB, Rennig M, Bertelsen AB, Ipsen JO,
            Brander S, Daley DO, Johansen KS, Norholm MHH
  TITLE     LyGo: A Platform for Rapid Screening of Lytic Polysaccharide 
            Monooxygenase Production.
  JOURNAL   ACS Synth Biol. 2021 Apr 16;10(4):897-906. doi: 
            10.1021/acssynbio.1c00034. Epub 2021 Apr 2.
  PUBMED    33797234
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00034"; journalName: "ACS Synth Biol"; date: 
            "2021-04-16- 16"; volume: "10"; issue: "4"; pages: "897-906"
COMMENT     Sequence Label: pLyGo-Kp-1
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(949..1005,1006..1203,1204..1215)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     CDS             complement(1231..1536)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDKSWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1333..1352)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      1757..2003
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(2416..4950)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(5363..6178)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5363..6178)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6086..6105
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6170..6189
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6343..6362)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    6557..7305
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(7341..7363)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7449..7589
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7504..7523
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7604..7621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7775..8363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7855..7874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8534..9325,9326..9394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9157..9176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9395..9499)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9567..9585
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
     1201 agagaggctg aagctcatcg aagagcttaa ttatattccc cagaacatca ggttaatggc
     1261 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
     1321 cggcacactg gccatatcgg tggtcatcat gcgccagctt ttatccccga tatgcaccac
     1381 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
     1441 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
     1501 gctctctctt ttataggtgt aaaccttaaa ctgcatagca tacctctttt tgacatactt
     1561 cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac
     1621 tgttatctgg cttttagtag cgaaaaaacc ccgccgaagc ggggtttttt gcggctcttc
     1681 gtaattcgcc ttagacatga ctgttcctca gttcaagttg ggcacttacg agaagaccgg
     1741 tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag atgcaggctt
     1801 catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt gtcattttgt
     1861 ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa tatcttgtgg
     1921 taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc actcctcttc
     1981 agagtacaga agattaagtg agacgttcgt ttgtgcaagc ttatcgataa gctttaatgc
     2041 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat
     2101 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
     2161 ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat
     2221 ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca
     2281 ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc
     2341 gactacgcga tcatggcgat cacacccgtc ctgtggatct atcgaatcta aatgtaagtt
     2401 aaaatctcta aataattaaa taagtcccag tttctccata cgaaccttaa cagcattgcg
     2461 gtgagcatct agaccttcaa cagcagccag atccatcact gcttggccaa tatgtttcag
     2521 tccctcagga gttacgtctt gtgaagtgat gaacttctgg aaggttgcag tgttaactcc
     2581 gctgtattga cgggcatatc cgtacgttgg caaagtgtgg ttggtaccgg aggagtaatc
     2641 tccacaactc tctggagagt aggcaccaac aaacacagat ccagcgtgtt gtacttgatc
     2701 aacataagaa gaagcattct cgatttgcag gatcaagtgt tcaggagcgt actgattgga
     2761 catttccaaa gcctgctcgt aggttgcaac cgatagggtt gtagagtgtg caatacactt
     2821 gcgtacaatt tcaacccttg gcaactgcac agcttggttg tgaacagcat cttcaattct
     2881 ggcaagctcc ttgtctgtca tatcgacagc caacagaatc acctgggaat caataccatg
     2941 ttcagcttga gacagaaggt ctgaggcaac gaaatctgga tcagcgtatt tatcagcaat
     3001 aactagaact tcagaaggcc cagcaggcat gtcaatacta cacagggctg atgtgtcatt
     3061 ttgaaccatc atcttggcag cagtaacgaa ctggtttcct ggaccaaata ttttgtcaca
     3121 cttaggaaca gtttctgttc cgtaagccat agcagctact gcctgggcgc ctcctgctag
     3181 cacgatacac ttagcaccaa ccttgtgggc aacgtagatg acttctgggg taagggtacc
     3241 atccttctta ggtggagatg caaaaacaat ttctttgcaa ccagcaactt tggcaggaac
     3301 acccagcatc agggaagtgg aaggcagaat tgcggttcca ccaggaatat agaggccaac
     3361 tttctcaata ggtcttgcaa aacgagagca gactacacca gggcaagtct caacttgcaa
     3421 cgtctccgtt agttgagctt catggaattt cctgacgtta tctatagaga gatcaatggc
     3481 tctcttaacg ttatctggca attgcataag ttcctctggg aaaggagctt ctaacacagg
     3541 tgtcttcaaa gcgactccat caaacttggc agttagttct aaaagggctt tgtcaccatt
     3601 ttgacgaaca ttgtcgacaa ttggtttgac taattccata atctgttccg ttttctggat
     3661 aggacgacga agggcatctt caatttcttg tgaggaggcc ttagaaacgt caattttgca
     3721 caattcaata cgaccttcag aagggacttc tttaggtttg gattcttctt taggttgttc
     3781 cttggtgtat cctggcttgg catctccttt ccttctagtg acctttaggg acttcatatc
     3841 caggtttctc tccacctcgt ccaacgtcac accgtacttg gcacatctaa ctaatgcaaa
     3901 ataaaataag tcagcacatt cccaggctat atcttccttg gatttagctt ctgcaagttc
     3961 atcagcttcc tccctaattt tagcgttcaa caaaacttcg tcgtcaaata accgtttggt
     4021 ataagaacct tctggagcat tgctcttacg atcccacaag gtggcttcca tggctctaag
     4081 accctttgat tggccaaaac aggaagtgcg ttccaagtga cagaaaccaa cacctgtttg
     4141 ttcaaccaca aatttcaagc agtctccatc acaatccaat tcgataccca gcaacttttg
     4201 agttgctcca gatgtagcac ctttatacca caaaccgtga cgacgagatt ggtagactcc
     4261 agtttgtgtc cttatagcct ccggaataga ctttttggac gagtacacca ggcccaacga
     4321 gtaattagaa gagtcagcca ccaaagtagt gaatagacca tcggggcggt cagtagtcaa
     4381 agacgccaac aaaatttcac tgacagggaa ctttttgaca tcttcagaaa gttcgtattc
     4441 agtagtcaat tgccgagcat caataatggg gattatacca gaagcaacag tggaagtcac
     4501 atctaccaac tttgcggtct cagaaaaagc ataaacagtt ctactaccgc cattagtgaa
     4561 acttttcaaa tcgcccagtg gagaagaaaa aggcacagcg atactagcat tagcgggcaa
     4621 ggatgcaact ttatcaacca gggtcctata gataacccta gcgcctggga tcatcctttg
     4681 gacaactctt tctgccaaat ctaggtccaa aatcacttca ttgataccat tattgtacaa
     4741 cttgagcaag ttgtcgatca gctcctcaaa ttggtcctct gtaacggatg actcaacttg
     4801 cacattaact tgaagctcag tcgattgagt gaacttgatc aggttgtgca gctggtcagc
     4861 agcataggga aacacggctt ttcctaccaa actcaaggaa ttatcaaact ctgcaacact
     4921 tgcgtatgca ggtagcaagg gaaatgtcat acttgaagtc ggacagtgag tgtagtcttg
     4981 agaaattctg aagccgtatt tttattatca gtgagtcagt catcaggaga tcctctacgc
     5041 cggacgcatc gtggccgacc tgcagggggg ggggggcgct gaggtctgcc tcgtgaagaa
     5101 ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag
     5161 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt
     5221 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa
     5281 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt
     5341 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     5401 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     5461 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     5521 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     5581 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
     5641 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     5701 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     5761 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     5821 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     5881 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     5941 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     6001 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     6061 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     6121 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     6181 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     6241 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttcc cccccccccc
     6301 tgcaggtcgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     6361 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     6421 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     6481 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     6541 cgcataaggg agagcgtcga gtatctatga ttggaagtat gggaatggtg atacccgcat
     6601 tcttcagtgt cttgaggtct cctatcagat tatgcccaac taaagcaacc ggaggaggag
     6661 atttcatggt aaatttctct gacttttggt catcagtaga ctcgaactgt gagactatct
     6721 cggttatgac agcagaaatg tccttcttgg agacagtaaa tgaagtccca ccaataaaga
     6781 aatccttgtt atcaggaaca aacttcttgt ttcgaacttt ttcggtgcct tgaactataa
     6841 aatgtagagt ggatatgtcg ggtaggaatg gagcgggcaa atgcttacct tctggacctt
     6901 caagaggtat gtagggtttg tagatactga tgccaacttc agtgacaacg ttgctatttc
     6961 gttcaaacca ttccgaatcc agagaaatca aagttgtttg tctactattg atccaagcca
     7021 gtgcggtctt gaaactgaca atagtgtgct cgtgttttga ggtcatcttt gtatgaataa
     7081 atctagtctt tgatctaaat aatcttgacg agccagacga taataccaat ctaaactctt
     7141 taaacgttaa aggacaagta tgtctgcctg tattaaaccc caaatcagct cgtagtctga
     7201 tcctcatcaa cttgaggggc actatcttgt tttagagaaa tttgcggaga tgcgatatcg
     7261 agaaaaaggt acgctgattt taaacgtgaa atttatctca agatctgctg cctcgcgcgt
     7321 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7381 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7441 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7561 gatgcgtaag gagaaaatac cgcatcaggc gctcaaccgc ttcctcgctc actgactcgc
     7621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8821 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     8881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9121 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9541 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     9601 aattaattct catgtttgac agcttatcat cgataagctg actcatgttg gtattgtgaa
     9661 atagacgcag atcgggaaca ctgaaaaata acagttatta ttcg
//
