LOCUS       sequence-338668-        3723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri ELAVL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-ELAVL2
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(139..166)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(258..344)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     393..415
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     407..424
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     408..424
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    440..539
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1719..1818)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1835..1854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1836..1854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1859..1875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1859..1875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1977..1996)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1988..2797
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2055..2074)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2944..3532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3686..3703
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      121 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      181 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
      241 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      301 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
      361 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
      421 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
      481 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctt
      541 cgaaggagat aacaaaatgg cagtcagact gtgcgatgtg gcttcactgc tcagaagtgg
      601 ttcgtgggcg cctgagcctt ggactggggt aattgctacc atggaaacac agctgtccaa
      661 cgggcccacg tgcaacaata cgagcaacgg tccttcaaca atctcaaaca actgctcctc
      721 gcccgtagag gggagcatag aggacagtaa aactaacttg atagtcaact atctgcccca
      781 gaacatgacc caggaggagc tgaagagttt gtttgggagc attggcgaaa tcgagtcctg
      841 taaacttgtt cgagataaaa taacgggaca gagcctagga tatggatttg tgaactacgt
      901 ggacccaaaa gatgcagaaa aagcaatcaa tactttaaac ggcttgagac ttcagaccaa
      961 aaccatcaag gtctcctacg cacgtccaag ctccgcctcc atcagagatg caaatctgta
     1021 cgtcagcggc ttgccaaaaa caatgacgca gaaagaactg gagcagctct tctcccagta
     1081 tggacgcatt attacctcac gcatcctggt ggaccaggtg accggtgtgt ccaggggggt
     1141 cggctttatt cgtttcgata ggcgggtcga ggctgaagag gccatcaagg gtttgaactg
     1201 ccagaagcca cctggcgcca ccgaacccat cacggtgaag tttgccaaca acccgagcca
     1261 gaagacgagc caggctctgc tgtcccagct gtatcagtcg cccaatcgaa ggtacccagg
     1321 acccctcgca cagcaggcac agcgcttcag gttggacaac ctgctgaaca tggcttatgg
     1381 agtcaaaagc aggttctccc caatggccat cgatggcgtg accagtttgg ccggcatcaa
     1441 catcccggga catgctggca ccggctggtg catcttcgtc tacaacctag ctccggacgc
     1501 agacgagagc atcctctggc agatgttcgg cccattcgga gccgtcacta acgtcaaggt
     1561 cattcgcgac ttcaacacaa acaagtgcaa aggattcggg ttcgtcacca tgactaatta
     1621 cgacgaggcc gctgtggcca tcgccagtct gaacggatac cgcctcgggg acagagttct
     1681 ccaagtctca ttcaaaacca acaaaacaca caaagccgac ccagctttct tgtacaaagt
     1741 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     1801 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     1861 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     1921 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     1981 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2041 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2101 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2161 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2221 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2281 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2341 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2401 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     2461 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     2521 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     2581 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     2641 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     2701 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     2761 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     2821 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     2881 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3721 aat
//
